ABSTRACT
We described the structure and analytical performance of a thin-film element for determining total hemoglobin in whole blood. The element can be used with the Kodak Ektachem DT60 analyzer. The method, analogous to the standard Drabkin's method, provides accurate and precise results on 10-microL samples of undiluted whole blood. Dynamic range is linear from 0 to 200 g/L, and the method is relatively free from interference.
Subject(s)
Hemoglobinometry/instrumentation , Autoanalysis , Humans , Methemoglobin/analogs & derivativesABSTRACT
The stimulation by calf serum of phosphate uptake into 3T3 cells results from a change in maximum velocity of the transport process with no change in the Michaelis constant. Only arsenate among a series of inorganic structural analogs of phosphate inhibited phosphate uptake indicating a high specificity for the process. The arsenate inhibition was competitive in nature. Papaverine, theophylline, and protaglandin E1, drugs known to maintain high intracellular levels of cAMP, had little effect on serum stimulated phosphate uptake. The phosphate uptake stimulating factor(s) in serum could be distinguised from the 3T3 cell survival and migration factors by stability characteristics, but this factor(s) could not be completely separated from a uridine uptake stimulation activity or growth promoting activity using a variety of serum fractionation procedures. Only partial stimulation of the uptake process was achieved with any one serum fraction indicating a multiplicity of serum components is probably involved in this process. Because of the rapidity of serum activation of phosphate uptake and its apparent independence of intracellular cyclic nucleotide levels, it is suggested that serum factors may stimulate phosphate uptake by inducing structural changes in the phosphate carrier system.
