Immunohistological determination of interleukin-1 beta in inflamed human gingival epithelium.

Interleukin-1 beta (Il-1 beta) is the predominant form of Il-1 produced by monocytes of the blood and by macrophages of various tissues. Human keratinocytes express both Il-1 alpha and Il-1 beta mRNA, but appear to produce mainly Il-1 alpha. The aim of this study was to determine the localization of interleukin-1 beta and interleukin-1 beta receptors in human gingival epithelium by different immunohistochemical methods. The alkaline phosphatase and the immunogold staining technique, as well as fluorescence microscopy, were used to investigate active participation of gingival epithelial cells in the development of periodontitis. Biopsies of human interdental papillae showed some activity of epithelial cells in the production of Il-1 beta. Single cells, clusters or larger areas of the sulcular and oral epithelium appeared to produce Il-1 beta at inflamed sites, and in these areas the normal epithelial structure was disturbed. Epithelial cells grown from the same biopsies appear able to express specific receptor molecules for Il-1 beta under normal culture conditions. It is concluded that gingival keratinocytes might be activated by inflammatory irritants and participate actively in the inflammatory processes.

Charge recombination reactions in photosystem II. 2. Transient absorbance difference spectra and their temperature dependence.

Absorbance difference spectra of the transient states in photosystem II (PS II) have been examined in the Qv absorption region between 660 and 700 nm. The P680+Pheo-/P680Pheo, 3P680/P680, and P680+QA-/P680QA spectra were measured in O2-evolving PS II core complexes from Synechococcus and PS II-enriched membrane fragments from spinach. The low-temperature absorbance difference spectra vary only slightly between both PS II preparations. The 3P680/P680 spectrum is characterized by a bleaching at 685 nm at 25 K and indicates weak exciton coupling with neighboring pigment(s). We conclude that P680 absorbs at 685 nm in more intact PS II preparations at cryogenic temperature. The difference spectra of the radical pairs are strongly temperature dependent. At low temperature the P680+QA-/P680QA- spectrum exhibits the strongest bleaching at 675 nm whereas the P680+Phe-/P680Pheo spectra show two distinct bleaching bands at 674 and 684 nm. It is suggested that an electrochronic red shift resulting in a bleaching at 675 nm and an absorbance increase at about 682 nm dominates the spectral features of the charge-separated states. On the basis of the present results and those in the literature, we conclude that the interactions between the pigments and especially the organization of the primary donor must be quite different in PS II compared to bacterial reaction centers, although the basic structural arrangement of the pigments might be similar. Spectral data obtained with samples in the presence of singly and doubly reduced QA indicate that the primary photochemistry in PS II is not strongly influenced by the redox state of QA at low temperature and confirm the results of the accompanying paper [Van Mieghem, F. J. E., Brettel, K., Hillmann, B., Kamlowski, A., Rutherford, A. W., & Schlodder, E. (1995) Biochemistry 34, 4798-4813]. The spectra of the primary radical pair and the reaction center triplet obtained with more intact PS II preparations differ widely from those of D1/D2/cyt b-559 complexes. In the latter sample, where 3P680 formation results in a bleaching at 680 nm, the P680+Pheo-/P680Pheo spectrum shows only one broad bleaching band at about 680 nm, and the main bleaching due to photoaccumulation of Pheo- at 77 K appears at 682 nm instead of 685 nm in PS II core complexes. This indicates that the removal of the core antenna which is accompanied by the loss of QA causes also structural changes of the reaction center.

Charge recombination reactions in photosystem II. I. Yields, recombination pathways, and kinetics of the primary pair.

Recombination reactions of the primary radical pair in photosystem II (PS II) have been studied in the nanosecond to millisecond time scales by flash absorption spectroscopy. Samples in which the first quinone acceptor (QA) was in the semiquinone form (QA-) or in the doubly reduced state (presumably QAH2) were used. The redox state of QA and the long-lived triplet state of the primary electron donor chlorophyll (3P680) were monitored by EPR. The following results were obtained at cryogenic temperatures (around 20 K). (1) the primary radical pair, P680+Pheo-, is formed with a high yield irrespective of the redox state of QA. (2) The decay of the primary pair is faster with QA- than with QAH2 and could be described biexponentially with t1/2 approximately 20 ns (approximately 65%)/150 ns (approximately 35%) and t1/2 approximately 60 ns (approximately 35%)/250 ns (approximately 65%), respectively. The different kinetics may be due to electrostatic and/or magnetic effects of QA- on charge recombination or due to conformational changes caused by the double reduction treatment. (3) The yield of the triplet state 3P680 was high both with QA- and QAH2. (4) The triplet decay was much faster with QA- [t1/2 approximately 2 microseconds (approximately 50%)/20 microseconds (approximately 50%)] than with QAH2 [t1/2 approximately 1 ms (approximately 65%)/3 ms (approximately 35%)]. The short lifetime of the triplet with QA- explains why it was not detected earlier. The mechanism of triplet quenching in the presence of QA- is not understood; however it may represent a protective process in PS II. (5) Almost identical data were obtained for PS II-enriched membranes from spinach and PS II core preparations from Synechococcus. Room temperature optical studies were performed on the Synechococcus preparation. In samples containing sodium dithionite to form QA- in the dark, EPR controls showed that multiple excitation flashes given at room temperature led to a decrease of the QA-Fe2+ signal, indicating double reduction of QA. During the first few flashes, QA- was still present in the large majority of the centers. In this case, the yield of the primary pair at room temperature was around 50%, and its decay could be described monoexponentially with t1/2 approximately 8 ns (a slightly better fit was obtained with two exponentials: t1/2 approximately 4 ns (approximately 80%)/25 ns (approximately 20%).(ABSTRACT TRUNCATED AT 400 WORDS)