Subject(s)
Play Therapy/methods , Psychotherapy, Group/methods , Adolescent , Female , Group Processes , Humans , MaleABSTRACT
Ten synthetic polymers of diphenolic compounds (KOP, HYKOP, CHOP, 3,4-DHTOP Na-ADROP, NH4-ADROP, Na-NORADROP, NH4-NORADROP, GENOP, and 2,5-DHTOP) as well as two phenolic polymers of natural origin (Na-humate, NH4-humate) were tested for their effectiveness on several strains of influenza virus type A and B. The allantois-on-shell system was used for primary screening. Virus multiplication was assessed by means of the infectious titer. All substances tested were found to exert inhibitory effects on influenza virus type A, while 3,4-DHTOP, Na-ADROP, NH4-ADROP as well as GENOP were effective against either type. The highest titer reductions (greater than 4.0 log 10 ID50) were obtained with KOP and HYKOP against influenzavirus A/Brasil/11/78 and with 3,4-DHTOP, KOP and HYKOP against influenzavirus A/Hongkong/1/68. Only GENOP showed antiviral activity against all virus strains tested so far.
Subject(s)
Influenza A virus/drug effects , Influenza B virus/drug effects , Phenols/pharmacology , DNA Replication/drug effects , Influenza A virus/genetics , Influenza A virus/growth & development , Influenza B virus/genetics , Influenza B virus/growth & development , Polymers , Species Specificity , Structure-Activity Relationship , Virus Replication/drug effectsABSTRACT
A method of haemagglutinin (HA) purification by means of K2-tartarate gradient centrifugation is described. Different influenza virus strains possessing the antigenic formulas H1N1, H2N2 and H3N2 yielded pure and immunologically active HA samples.
