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1.
J Biochem ; 171(4): 459-465, 2022 Mar 31.
Article in English | MEDLINE | ID: mdl-35081614

ABSTRACT

A tRNA-like sequence conserved in the genomes of all Escherichia coli strains was found. The sequence resembles arginine-tRNA, which is present in E. coli pathogenic islands and phages. Expression experiments revealed that this sequence is a part of a leaderless mRNA encoding a short peptide (60 amino acids: XtpA). A deletion mutant of this gene is more sensitive than wild-type cell to several aminoglycoside antibiotics at low concentrations. Further analyses indicated that XtpA positively regulates the expression of GcvB small RNA, which is involved in the intrinsic resistance to aminoblycosides in E. coli.


Subject(s)
Escherichia coli , RNA, Bacterial , Escherichia coli/metabolism , Gene Expression Regulation, Bacterial , Peptides/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , RNA, Transfer/genetics , RNA, Transfer/metabolism
2.
J Biochem ; 169(4): 485-489, 2021 Apr 29.
Article in English | MEDLINE | ID: mdl-33169170

ABSTRACT

Deleting the gene for small RNA GcvB in Escherichia coli was found to increase the sensitivity to several aminoglycoside antibiotics, such as neomycin, streptomycin, kanamycin, kasugamycin and spectinomycin, at low concentrations. GcvB, conserved in gram-negative enteric bacteria, is known to negatively control the expression of many genes for amino acid incorporation systems, especially the periplasmic ABC-transporter proteins. Deletions of several amino acid transporter genes in ΔgcvB cells decreased the antibiotic sensitivity to the wild-type level, suggesting that those genes are involved in uptake of aminoglycosides into the cell. Since GcvB is constitutively synthesized in growing cells, repressing synthesis of amino acid transporters, it contributes to the intrinsic resistance to several aminoglycoside antibiotics.


Subject(s)
Aminoglycosides , Drug Resistance, Bacterial , Escherichia coli , Gene Expression Regulation, Bacterial , RNA, Bacterial , RNA, Untranslated , Escherichia coli/genetics , Escherichia coli/metabolism , RNA, Bacterial/biosynthesis , RNA, Bacterial/genetics , RNA, Untranslated/biosynthesis , RNA, Untranslated/genetics
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