New High-Resolution Analysis of the nu(3) Band of the (15)N(16)O(2) Isotopomer of Nitrogen Dioxide by Fourier Transform Spectroscopy.

New high-resolution Fourier transform absorption spectra of an (15)N(16)O(2) isotopic sample of nitrogen dioxide were recorded at the University of Bremen in the 6.3-µm region. Starting from the results of a previous study [Y. Hamada, J. Mol. Struct. 242, 367-377 (1991)], a new and more extended analysis of the nu(3) band located at 1582.1039 cm(-1) has been performed. The spin-rotation energy levels were satisfactorily reproduced using a theoretical model which takes into account both the Coriolis interactions between the spin-rotation energy levels of the (001) vibrational state with those of the (020) and (100) states and the spin-rotation resonances within each of the NO(2) vibrational states. Precise vibrational energies and rotational, spin-rotation, and coupling constants were obtained in this way for the first triad of (15)N(16)O(2) interacting states {(020), (100), (001)}. Finally, a comprehensive list of line positions and line intensities of the {nu(1), 2nu(2), nu(3)} interacting bands of (15)N(16)O(2) was generated, using for the line intensities the transition moment operators which were obtained previously for the main isotopic species. Copyright 2000 Academic Press.

Restoring and immunohistological examination of Feulgen stained avian embryonic tissues using iron hematoxylin and endothelial cell specific antibody.

The immunohistological method described here permits re-examination of previously Feulgen stained quail-chick chimera tissues for vascular development using the monoclonal antibody QH1 which specifically recognizes quail hemangioblastic cells. Weigert's iron hematoxylin has been used to restore faded or bleached Feulgen stained chimeric avian tissues. Species-specific differences in nuclear morphology are as evident with iron hematoxylin staining as they are with Feulgen staining.