Pollen report: quantitative review of pollen crude protein concentrations offered by bee pollinated flowers in agricultural and non-agricultural landscapes.

To ease nutritional stress on managed as well as native bee populations in agricultural habitats, agro-environmental protection schemes aim to provide alternative nutritional resources for bee populations during times of need. However, such efforts have so far focused on quantity (supply of flowering plants) and timing (flower-scarce periods) while ignoring the quality of the two main bee relevant flower-derived resources (pollen and nectar). As a first step to address this issue we have compiled one geographically explicit dataset focusing on pollen crude protein concentration, one measurement traditionally associated with pollen quality for bees. We attempt to provide a robust baseline for protein levels bees can collect in- (crop and weed species) and off-field (wild plants) in agricultural habitats around the globe. Using this dataset we identify crops which provide sub-optimal pollen resources in terms of crude protein concentration for bees and suggest potential plant genera that could serve as alternative resources for protein. This information could be used by scientists, regulators, bee keepers, NGOs and farmers to compare the pollen quality currently offered in alternative foraging habitats and identify opportunities to improve them. In the long run, we hope that additional markers of pollen quality will be added to the database in order to get a more complete picture of flower resources offered to bees and foster a data-informed discussion about pollinator conservation in modern agricultural landscapes.

The nectar report: quantitative review of nectar sugar concentrations offered by bee visited flowers in agricultural and non-agricultural landscapes.

There is growing concern that some bee populations are in decline, potentially threatening pollination security in agricultural and non-agricultural landscapes. Among the numerous causes associated with this trend, nutritional stress resulting from a mismatch between bee nutritional needs and plant community provisioning has been suggested as one potential driver. To ease nutritional stress on bee populations in agricultural habitats, agri-environmental protection schemes aim to provide alternative nutritional resources for bee populations during times of need. However, such efforts have focused mainly on quantity (providing flowering plants) and timing (during flower-scarce periods), while largely ignoring the quality of the offered flower resources. In a first step to start addressing this information gap, we have used literature data to compile a comprehensive geographically explicit dataset on nectar quality (i.e., total sugar concentration), offered to bees both within fields (crop and weed species) as well as outside fields (wild species) around the globe. Social bees are particularly sensitive to nectar sugar concentrations, which directly impact calorie influx into the colony and consequently their fitness making it an important resource quality marker. We find that the total nectar sugar concentrations in general do not differ between the three plant communities studied. In contrast we find increased variability in nectar quality in the wild plant community compared to crop and weed community, which is likely explained by the increased phylogenetic diversity in this category of plants. In a second step we explore the influence of local habitat on nectar quality and its variability utilizing a detailed sunflower (Helianthus annuus L.) data set and find that geography has a small, but significant influence on these parameters. In a third step we identify crop groups (genera), which provide sub-optimal nectar resources for bees and suggest high quality alternatives as potential nectar supplements. In the long term this data set could serve as a starting point to systematically collect more quality characteristics of plant provided resources to bees, which ultimately can be utilized by scientist, regulators, NGOs and farmers to improve the flower resources offered to bees. We hope that ultimately this data will help to ease nutritional stress for bee populations and foster a data informed discussion about pollinator conservation in modern agricultural landscapes.

Dopamine Receptor DAMB Signals via Gq to Mediate Forgetting in Drosophila.

Prior studies have shown that aversive olfactory memory is acquired by dopamine acting on a specific receptor, dDA1, expressed by mushroom body neurons. Active forgetting is mediated by dopamine acting on another receptor, Damb, expressed by the same neurons. Surprisingly, prior studies have shown that both receptors stimulate cyclic AMP (cAMP) accumulation, presenting an enigma of how mushroom body neurons distinguish between acquisition and forgetting signals. Here, we surveyed the spectrum of G protein coupling of dDA1 and Damb, and we confirmed that both receptors can couple to Gs to stimulate cAMP synthesis. However, the Damb receptor uniquely activates Gq to mobilize Ca2+ signaling with greater efficiency and dopamine sensitivity. The knockdown of Gαq with RNAi in the mushroom bodies inhibits forgetting but has no effect on acquisition. Our findings identify a Damb/Gq-signaling pathway that stimulates forgetting and resolves the opposing effects of dopamine on acquisition and forgetting.