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2.
Science ; 292(5525): 2269, 2001 Jun 22.
Article in English | MEDLINE | ID: mdl-11423645
5.
Science ; 269(5222): 283, 1995 Jul 21.
Article in English | MEDLINE | ID: mdl-17841225
6.
Science ; 266(5185): 523, 1994 Oct 28.
Article in English | MEDLINE | ID: mdl-7939691
7.
Science ; 264(5167): 1827, 1994 Jun 24.
Article in English | MEDLINE | ID: mdl-8009199
9.
Cell ; 30(2): 459-68, 1982 Sep.
Article in English | MEDLINE | ID: mdl-6291769

ABSTRACT

Initiation of DNA replication at specific origins was observed by electron microscopy after microinjection of pXlr11, pXlr14 or Col E1 plasmid DNA molecules into unfertilized eggs of the frog, Xenopus laevis. These results are in apparent contradiction with published reports (Harland and Laskey, Cell 21, 761-771, 1980; Laskey and Harland, Cell 24, 283-284, 1981) that specific origin sites were not used in Xenopus laevis eggs. We suggest that eucaryotic origins exist that both increase the probability of replication of contiguous sequences and determine the site at which replication is most likely to begin.


Subject(s)
DNA Replication , DNA/metabolism , Ovum/metabolism , Plasmids , Animals , Bacteriocin Plasmids , Base Sequence , DNA Restriction Enzymes , DNA, Recombinant , DNA, Ribosomal , Female , Microinjections , Microscopy, Electron , Xenopus laevis
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