Measuring Image Resolution in Ultrasound Localization Microscopy.

The resolution of an imaging system is usually determined by the width of its point spread function and is measured using the Rayleigh criterion. For most system, it is in the order of the imaging wavelength. However, super resolution techniques such as localization microscopy in optical and ultrasound imaging can resolve features an order of magnitude finer than the wavelength. The classical description of spatial resolution no longer applies and new methods need to be developed. In optical localization microscopy, the Fourier Ring Correlation has showed to be an effective and practical way to estimate spatial resolution for Single Molecule Localization Microscopy data. In this work, we wish to investigate how this tool can provide a direct and universal estimation of spatial resolution in Ultrasound Localization Microscopy. Moreover, we discuss the concept of spatial sampling in Ultrasound Localization Microscopy and demonstrate how the Nyquist criterion for sampling drives the spatial/temporal resolution tradeoff. We measured spatial resolution on five different datasets over rodent's brain, kidney and tumor finding values between [Formula: see text] and [Formula: see text] for precision of localization between [Formula: see text] and [Formula: see text]. Eventually, we discuss from those in vivo datasets how spatial resolution in Ultrasound Localization Microscopy depends on both the localization precision and the total number of detected microbubbles. This study aims to offer a practical and theoretical framework for image resolution in Ultrasound Localization Microscopy.

A versatile and robust microfluidic device for capillary-sized simple or multiple emulsions production.

Ultrasound-vaporizable microdroplets can be exploited for targeted drug delivery. However, it requires customized microfluidic techniques able to produce monodisperse, capillary-sized and biocompatible multiple emulsions. Recent development of microfluidic devices led to the optimization of microdroplet production with high yields, low polydispersity and well-defined diameters. So far, only few were shown to be efficient for simple droplets or multiple emulsions production below 5 µm in diameter, which is required to prevent microembolism after intravenous injection. Here, we present a versatile microchip for both simple and multiple emulsion production. This parallelized system based on microchannel emulsification was designed to produce perfluorocarbon in water or water within perfluorocarbon in water emulsions with capillary sizes (<5 µm) and polydispersity index down to 5% for in vivo applications such as spatiotemporally-triggered drug delivery using Ultrasound. We show that droplet production at this scale is mainly controlled by interfacial tension forces, how capillary and viscosity ratios influence droplet characteristics and how different production regimes may take place. The better understanding of droplet formation and its relation to applied pressures is supported by observations with a high-speed camera. Compared to previous microchips, this device opens perspectives to produce injectable and biocompatible droplets with a reasonable yield in order to realize preclinical studies in mice.