Design and fabrication of polysaccharide based excellent chemical resistant and UV barrier ternary blend films for green packaging applications.

The development of green materials for active packaging applications is a research hotspot due to setbacks of petrochemical derived plastics. Thus, the present study aims to develop ternary blend films by doping different wt% of Tragacanth gum (TG) to Poly(vinyl alcohol)/Chitosan (PC) blend using solvent evaporation technique. Further, their various physicochemical properties were evaluated systematically. Differential scanning calorimetry studies revealed excellent compatibility and thermal stability of PC blend was significantly reinforced with 15 wt% of TG. UV-visible spectroscopy study demonstrated the excellent shielding efficacy of UV radiation by ternary blend films. Moreover, overall migration results confirmed the limited release of film constituents into food simulants and swelling ratio analysis indicated the good swelling resistance at higher wt% of TG. The ternary films exhibited tremendous chemical resistance against extreme acidic and basic environments and these green biofilms could be considered for active packaging applications.

Evaluation of mechanical, antimicrobial, and antioxidant properties of vanillic acid induced chitosan/poly (vinyl alcohol) active films to prolong the shelf life of green chilli.

Vanillic acid incorporated chitosan/poly(vinyl alcohol) active films were prepared by employing a cost-effective solvent casting technique. FTIR investigation validated the intermolecular interaction and formation of Schiff's base (C=N) between functional groups of vanillic acid, chitosan, and poly(vinyl alcohol). The addition of vanillic acid resulted in homogenous and dense morphology, as confirmed by SEM micrographs. The tensile strength of active films increased from 32 to 59 MPa as the amount of vanillic acid increased and the obtained values are more significant than reported polyethylene (2231 MPa) and polypropylene (31-38 MPa) films, widely utilized in food packaging. Active film's UV, water, and oxygen barrier properties exhibited excellent results with the incorporation of vanillic acid. Around 40 % of degradation commences within 15 days. Synergistic impact against S. aureus, E. coli, and C. albicans pathogens caused the expansion of the inhibition zone, evidenced by the excellent antimicrobial activity. The highest antioxidant capacity, 73.65 % of CPV-4 active film, proved that active films could prevent the spoilage of food from oxidation. Green chillies packaging was carried out to examine the potential of prepared active films as packaging material results in successfully sustaining carotenoid accumulation and prolonging the shelf life compared to conventional polyethylene (PE) packaging.

Basella alba stem extract integrated poly (vinyl alcohol)/chitosan composite films: A promising bio-material for wound healing.

Natural extract-based bio-composite material for wound healing is gaining much attention due to risk of infection and high cost of commercial wound dressing film causes serious problem on the human well-being. Herein, the study outlines the preparation of Poly (vinyl alcohol)/Chitosan/Basella alba stem extract (BAE) based bio-composite film through solvent casting technique and well characterized for wound healing application. Incorporation of BAE into Poly (vinyl alcohol)/Chitosan matrix has shown existence of secondary interactions confirmed by FT-IR analysis. Good morphology, thermal stability and significant improvement in flexibility (∼63.38 %) of the films were confirmed by SEM, TGA and Mechanical test results, respectively. Hydrophilic property (∼9.04 %), water vapor transmission rate (∼70.07 %), swelling ability (∼14.7 %) and degradation rate (∼14.04 %) were enhanced with increase in BAE content. In-vitro studies have shown good antibacterial activity against foremost infectious bacterial strains S. aureus and E. coli. Additionally, BAE integrated Poly (vinyl alcohol)/Chitosan film has amplified anti-inflammatory (∼79.38 %) property, hemocompatibility and excellent biocompatibility (94.9 %) was displayed by cytotoxicity results. Moreover, in-vitro scratch assay and cell adhesion test results illustrated prominent wound healing (96.5 %) and adhesion. Overall results of the present work proclaim that developed bio-composite film could be utilized as a biomaterial in wound care applications.

Chitosan/pullulan based films incorporated with clove essential oil loaded chitosan-ZnO hybrid nanoparticles for active food packaging.

Herein, we developed clove essential oil (CEO) loaded Chitosan-ZnO hybrid nanoparticles (CS-ZnO@CEO (CZC NPs)) integrated chitosan/pullulan (CS/PL) nanocomposite films. SEM images revealed a homogenous distribution of CZC NPs with minimum aggregation in nanocomposite films. The incorporation of CZC NPs led to enhanced tensile strength (~39.82%), film hydrophobicity (~35.36%), UV light blocking ability, water vapor barrier (~84.64%), and oxygen barrier (~57.66%) compared to the bare CS/PL film and overall migration limit of CPCZC films were found below the permitted limit of 1000 µg/dm2. Besides, incorporation of CZC NPs into the CS/PL films enhanced antioxidant activity and showed strong antibacterial activity against P. aeruginosa, S. aureus, and E. coli. Also, the CPCZC films displayed potential to extend the shelf-life of chicken meat by up to 5 days when stored at 8 ± 2 °C. These results suggest that the prepared CPCZC films acquire the ideal prerequisites for potential active packaging materials.

Preparation and physicochemical assessment of bioactive films based on chitosan and starchy powder of white turmeric rhizomes (Curcuma Zedoaria) for green packaging applications.

In the current study, the bioactive films of chitosan/white turmeric (CH/WT) were prepared by employing solvent casting technique and analyzed their physicochemical and biological properties for active packaging applications. The successful inclusion of white turmeric into the chitosan matrix is confirmed by Fourier Transform Infrared Spectroscopy. Due to the presence of hydrogen bonding interaction, the active films exhibited good tensile properties, smooth surface morphology, miscibility, water resistance and UV barrier properties. The incorporation of white turmeric reduced the water vapour transmission rate and oxygen permeability (p < 0.05) in contrast with pristine film. The prepared blend films revealed soil degradation rate more than 60% within 15 days. Furthermore, the blend films exhibited lesser water solubility, moisture content and swelling index after addition of white turmeric to chitosan (p < 0.05). The prepared films revealed extensive antimicrobial activity against gram positive and gram negative bacteria. The antioxidant activity and total phenolic content were improved upon the incorporation of white turmeric. Moreover, the oil absorption rate of the blend films was decreased by 46% in comparison with pristine film. Overall, white turmeric incorporated chitosan films were employed as a green packaging material to extend the shelf life of the foodstuff.

Smart biodegradable films based on chitosan/methylcellulose containing Phyllanthus reticulatus anthocyanin for monitoring the freshness of fish fillet.

The current work aims to prepare biologically active and pH responsive smart films based on Chitosan (CS)/Methylcellulose (MC) matrix integrated with Phyllanthus reticulatus (PR) ripen fruit anthocyanin. The prepared smart films (CMPR) were fabricated through a cost-effective solvent casting technique. The existences of secondary interactions were confirmed by the FT-IR analysis. The smooth SEM images revealed the miscibility and compatibility of the CS/MC matrix with PR anthocyanin. The incorporation of PR anthocyanin significantly blocked the UV light transmission of the CS/MC films while slight decrease in the transparency was observed. The water solubility, moisture retention capacity, and water vapor transmission rate were significantly enhanced with an increase in the PR anthocyanin content. Additionally, the prepared CMPR smart films showed pink color in acidic pH while yellowish in basic pH solution and further exhibited strong antioxidant activity as well as antibacterial activity against the common foodborne pathogens such as S. aureus, P. aeruginosa, and E. coli. The CMPR smart film also displayed potential result for monitoring the fish fillet freshness at room temperature. The results proclaim that the prepared CMPR smart films could be utilized for quality assurance as well as shelf life extension of the marine food products.