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1.
Clin Infect Dis ; 73(7): e1719-e1726, 2021 10 05.
Article in English | MEDLINE | ID: mdl-32569354

ABSTRACT

BACKGROUND: Helicobacter pylori is primarily an extracellularly living bacterium. However, seemingly intracellular occurrence can often be detected by immunohistochemical stains. Considering antimicrobial resistance, we investigated the impact of the apparent intracellular H. pylori (aiHp) on treatment failure of first-line triple therapies. METHODS: Gastric biopsies of 814 patients infected with H. pylori naive to treatment were analyzed before and after eradication therapy by immunohistochemistry. Of these, 373 received treatment consisting of amoxicillin, clarithromycin, and proton pump inhibitor (AC/PPI). Availability of polymerase chain reaction-based clarithromycin susceptibility test results from pretreatment gastric biopsies was a precondition for matching 52 aiHp to 52 non-aiHp cases within the AC/PPI group. RESULTS: AiHp were detected mostly in low counts predominantly in corpus biopsies, rarely in antrum biopsies (95.2% vs 24.6%); they were found in 497 (61%) of all patients and in 192 of 373 patients (51.5%) in the AC/PPI group. The eradication rate in aiHp versus non-aiHp cases was 44.4% versus 72.9% in the entire sample and 45.3% versus 66.8% in the AC/PPI group. Among the 104 paired patients, respective values were 46.2% versus 78.8%; in clarithromycin-susceptible cases, 60.6% versus 91.9%. Both aiHp and resistance to clarithromycin proved to be highly significant (P ≤ .001) and independent predictors of eradication failure. Twelve of 13 aiHp cases with a clarithromycin-sensitive strain who failed eradication developed resistance to the antibiotic. CONCLUSIONS: AiHp found by immunohistochemical staining especially in corpus biopsies proved to be a risk factor for failure of first-line triple therapies; occurrence of aiHp should be considered with regard to therapy options.


Subject(s)
Helicobacter Infections , Helicobacter pylori , Amoxicillin/therapeutic use , Anti-Bacterial Agents/therapeutic use , Clarithromycin/therapeutic use , Drug Therapy, Combination , Helicobacter Infections/drug therapy , Humans , Immunohistochemistry , Metronidazole/therapeutic use , Proton Pump Inhibitors/therapeutic use
2.
BMC Health Serv Res ; 20(1): 1105, 2020 Dec 01.
Article in English | MEDLINE | ID: mdl-33256736

ABSTRACT

BACKGROUND: Anemia is a risk factor for adverse outcomes, which can be aggravated by unnecessary phlebotomies. In blood culture testing, up to 30 ml of blood can be withdrawn per sample, even though most manufacturers recommend blood volumes of 10 ml or less. After assessing the filling volume of blood culture bottles at our institution, we investigated whether an educational intervention could optimize filling volume of blood culture bottles without negatively affecting microbiology testing. METHODS: We weighed 10,147 blood cultures before and 11,806 blood cultures after a six-month educational intervention, during which employees were trained regarding correct filling volume via lectures, handouts, emails, and posters placed at strategic places. RESULTS: Before the educational intervention, only 31% of aerobic and 34% of anaerobic blood cultures were filled correctly with 5-10 ml of blood. The educational intervention increased the percentage of correctly filled bottles to 43% (P < 0.001) for both aerobic and anaerobic samples without negatively affecting results of microbiologic testing. In addition, sample volume was reduced from 11.0 ± 6.5 to 9.4 ± 5.1 ml (P < 0.001) in aerobic bottles and from 10.1 ± 5.6 to 8.8 ± 4.8 ml (P < 0.001) in anaerobic bottles. CONCLUSION: Education of medical personnel is a simple and effective way to reduce iatrogenic blood loss and possibly moderate the extent of phlebotomy-induced anemia.


Subject(s)
Blood Culture , Phlebotomy , Bacteriological Techniques , Humans
3.
Helicobacter ; 24 Suppl 1: e12641, 2019 Sep.
Article in English | MEDLINE | ID: mdl-31486244

ABSTRACT

Endoscopic imaging of the stomach is improving. In addition to narrow band imaging, other methods, for example, blue light imaging and linked color imaging, are now available and can be combined with artificial intelligence systems to obtain information on the gastric mucosa and detect early gastric cancer. Immunohistochemistry is only recommended as an ancillary stain in case of chronic active gastritis without Helicobacter pylori detection by standard staining, and recommendations to exclude false negative H. pylori results have been made. Molecular methods using real-time PCR, droplet digital PCR, or amplification refractory mutation system PCR have shown a high accuracy, both for detecting H. pylori and for clarithromycin susceptibility testing, and can now be used in clinical practice for targeted therapy. The most reliable non-invasive test remains the 13 C-urea breath test. Large data sets show that DOB values are higher in women and that the cut-off for positivity could be decreased to 2.74 DOB. Stool antigen tests using monoclonal antibodies are widely used and may be a good alternative to UBT, particularly in countries with a high prevalence of H. pylori infection. Attempts to improve serology by looking at specific immunodominant antigens to distinguish current and past infection have been made. The interest of Gastropanel® which also tests pepsinogen levels was confirmed.


Subject(s)
Breath Tests/methods , Diagnostic Tests, Routine/methods , Endoscopy, Gastrointestinal/methods , Helicobacter Infections/diagnosis , Immunoassay/methods , Molecular Diagnostic Techniques/methods , Diagnostic Tests, Routine/trends , Endoscopy, Gastrointestinal/trends , Humans , Immunoassay/trends , Molecular Diagnostic Techniques/trends
4.
Sci Rep ; 8(1): 16024, 2018 10 30.
Article in English | MEDLINE | ID: mdl-30375435

ABSTRACT

This study analyzed the performance of different molecular technologies along with blood culture (BC) in the diagnosis of bloodstream infections (BSI) in patients from internal medicine wards - including intensive care units (ICUs) - and the emergency room. Patients with systemic inflammatory response syndrome were prospectively included. BCs and EDTA whole blood were obtained simultaneously. The latter was analyzed by PCR combined with electrospray ionization mass spectrometry (PCR/ESI-MS; IRIDICA BAC BSI assay, Abbott) and by SeptiFast (Roche). Cases were classified as BSI according to adapted European Centre for Disease Prevention and Control criteria. Out of 462 analyzed episodes, 193 with valid test results fulfilled the inclusion criteria and were further evaluated. Sixty-nine (35.8%) were classified as BSI. PCR/ESI-MS showed a significantly better overall performance than BC (p = 0.004) or SeptiFast (p = 0.034). Only in patients from the ICU the performance of SeptiFast was comparable to that of PCR/ESI-MS. Mainly due to the negative effect of antimicrobial pre-treatment on BC results, the cumulative performance of each of the molecular tests with BC was significantly higher than that of BC alone (p < 0.001). SeptiFast and in particular the broad-range pathogen detection system PCR/ESI-MS proved to be an essential addition to BC-based diagnostics in BSI.


Subject(s)
Blood Culture , Polymerase Chain Reaction , Sepsis/diagnosis , Spectrometry, Mass, Electrospray Ionization , Blood Culture/methods , Blood Culture/standards , Female , Humans , Male , Polymerase Chain Reaction/methods , Polymerase Chain Reaction/standards , Reproducibility of Results , Sensitivity and Specificity , Sepsis/blood , Sepsis/etiology , Sepsis/therapy , Spectrometry, Mass, Electrospray Ionization/methods , Spectrometry, Mass, Electrospray Ionization/standards
5.
J Med Microbiol ; 66(10): 1383-1392, 2017 Oct.
Article in English | MEDLINE | ID: mdl-28884671

ABSTRACT

PURPOSE: The rise in the incidence of fungal infections and the expanding spectrum of fungal pathogens make early and broad detection of fungal pathogens essential. In the present study, a panfungal real-time PCR assay for the broad-range detection of fungal DNA (Fungi assay) in a wide variety of clinical specimens was developed. METHODOLOGY: Our in-house, HybProbe real-time PCR assay targets the ITS2 region of fungal DNA. The applicability was evaluated by testing 105 clinical samples from 98 patients with suspected fungal infection. Samples included tissue biopsies, paraffin embedded tissues, aspirates, EDTA-anticoagulated blood, cerebrospinal fluids and bronchoalveolar lavages. RESULTS: Fungal pathogens were identified by the Fungi assay in 47 samples. In all of these cases, conventional methods and clinical data were also indicative for a fungal infection. Five samples were interpreted false negative. blast analyses of the amplicons derived from 11 samples revealed the presence of environmental fungal species while other tests and clinical data did not suggest a fungal infection. This fact might indicate contaminated samples. The remaining 42 samples were negative by the Fungi assay as well as the conventional methods and were therefore regarded as true negatives. Thus, sensitivity was 90.4 % and specificity 79.2 %. CONCLUSION: The Fungi assay improved the targeted diagnosis of fungal infections allowing pathogen identification in samples that were histologically positive but culture negative. For reliable diagnosis, results have to be interpreted in context with conventional methods and clinical data.


Subject(s)
DNA, Fungal/isolation & purification , DNA, Intergenic/genetics , Mycoses/diagnosis , Mycoses/microbiology , Real-Time Polymerase Chain Reaction/methods , DNA, Fungal/genetics , Humans , Sensitivity and Specificity
6.
Wien Klin Wochenschr ; 129(11-12): 427-434, 2017 Jun.
Article in English | MEDLINE | ID: mdl-28243751

ABSTRACT

BACKGROUND: In recent years a multiplex real-time PCR (SeptiFast) has been introduced, allowing detection of 25 common blood pathogens considerably faster than conventional blood culture. METHODS: SeptiFast was applied routinely in addition to blood culture in cases of critically ill patients with fever and other signs of severe systemic infections. In this study data of 470 episodes were retrospectively analysed to assess the impact of various parameters, such as clinical indications, assigning ward and antimicrobial treatment on test outcome using a multivariate logistic model. RESULTS: After exclusion of microorganisms classified as contaminants, the concordance between SeptiFast and blood culture was 85.5%. SeptiFast detected 98 out of 120, while blood culture merely found 63 out of 120 potential pathogens. In comparison to blood culture, SeptiFast showed considerably higher positivity rates in sepsis, pneumonia and febrile immunosuppression and a lower rate in endocarditis. The highest positivity and concordance between tests was shown in patients from the emergency room (P = 0.007). CONCLUSIONS: The results obtained in this study are similar to those from prospective settings confirming the robustness of the SeptiFast assay in routine use. Our data suggest that SeptiFast is a valuable add-on to blood culture and may increase the diagnostic efficiency of a microbiological laboratory.


Subject(s)
Bacteremia/blood , Bacteremia/diagnosis , Blood Culture/methods , Diagnostic Tests, Routine/methods , Polymerase Chain Reaction/methods , Sepsis/blood , Sepsis/diagnosis , Adult , Aged , Bacteremia/microbiology , Female , Humans , Longitudinal Studies , Male , Middle Aged , Reproducibility of Results , Sensitivity and Specificity , Sepsis/microbiology , Young Adult
7.
J Orthop Res ; 35(7): 1383-1387, 2017 07.
Article in English | MEDLINE | ID: mdl-27572456

ABSTRACT

Modular megaprostheses are known for high infection rates followed by high rates of revisions. Microbial biofilms growing adherently on prosthetic surfaces may inhibit the detection of the pathogens causing prosthetic joint infections. We sought to answer the following questions: Does sonication culture (SC) improve the microbiological diagnosis of periprosthetic infections of megaprostheses compared to conventional tissue culture (TC)? Which pathogens were detected on the surface of megaprostheses with either SC or TC and do the findings help to identify low-grade infections? Included were 31 patients with modular megaprostheses, whose implant had been explanted due to suspected joint infection or revision surgery. SCs were performed according to the protocol by Trampuz et al. The diagnosis of infection was evaluated according to the definition of the Musculoskeletal Infection Society. The sensitivity of SC was 91.3% compared to 52.2% for TC and the specificity was 100% for SC and TC (p = 0.004). Under preoperative antibiotic therapy, the sensitivity of SC was 83.3% while the sensitivity of TC was 50%. Without preoperative antibiotic therapy the sensitivity of SC was 100% compared to 54.5% for TC. In nine cases, SCs detected microorganisms, while TC was negative. Detected bacteria were Staphylococcus epidermidis in four, Micrococcus species in one, Finegoldia magna in one, Brevibacterium casei in one, Pseudomonas fluorescens in one, and Enterococcus faecium in one. SC is a reliable method for dislodging pathogens from orthopedic implants. The SC of modular megaprostheses showed significantly higher pathogen detection than the periprosthetic TC, especially for low virulence pathogens. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:1383-1387, 2017.


Subject(s)
Prosthesis-Related Infections/diagnosis , Prosthesis-Related Infections/microbiology , Sonication , Tissue Culture Techniques , Humans
9.
Liver Int ; 35(9): 2121-8, 2015 Sep.
Article in English | MEDLINE | ID: mdl-25644943

ABSTRACT

BACKGROUND: Patients with ascites are at risk for developing spontaneous bacterial peritonitis (SBP) - a severe complication associated with high mortality. We aimed to identify risk factors for SBP development and mortality to optimize stratification for primary prophylaxis and therapeutic strategies to improve survival. METHODS: 575 patients with cirrhosis and ascites undergoing paracentesis at a tertiary care hospital were included in this retrospective cohort study. Demographical, clinical and laboratory parameters were recorded at first paracentesis and during follow-up. Multivariate logistic regression analysis was used to identify independent predictors of SBP development and mortality. RESULTS: Child-Pugh stage C (OR: 3.323; P = 0.009), ascitic fluid polymorph-nuclear cell (PMN) count (OR: 1.544; P = 0.028) and low serum sodium (OR: 0.917; P = 0.029) emerged as independent risk factors for SBP development. SBP-naïve patients undergoing paracentesis and presenting with PMN-counts ≥100 cells/µl, or hyponatraemia <125 mM were at highest risk for developing SBP. Increases in MELD and CRP levels indicated SBP development, while no changes where observed in a matched control group with sterile ascites at multiple paracenteses. MELD score (OR: 1.565; P = 0.001) and CRP (OR: 1.067; P = 0.037) were identified as independent risk factors for 30-day mortality after SBP diagnosis. In particular SBP patients with MELD≥22, CRP ≥3.5 mg/dl and development of grade III/IV hepatic encephalopathy showed highest mortality. CONCLUSIONS: Low serum sodium levels, Child-Pugh stage C and elevated ascites PMN counts (≥100 cells/µl) indicate a significant risk for SBP development. SBP-related mortality is highest in patients with MELD≥22 and elevated CRP levels.


Subject(s)
Ascites/etiology , Bacterial Infections/complications , Liver Cirrhosis/complications , Peritonitis/diagnosis , Peritonitis/mortality , Aged , Ascites/complications , Ascitic Fluid/microbiology , Austria , Female , Humans , Kaplan-Meier Estimate , Logistic Models , Male , Middle Aged , Multivariate Analysis , Paracentesis , Peritonitis/microbiology , Retrospective Studies , Risk Factors , Sodium/blood , Tertiary Care Centers
10.
Infect Genet Evol ; 27: 408-17, 2014 Oct.
Article in English | MEDLINE | ID: mdl-25159028

ABSTRACT

The detailed analysis of antibiotic resistance mechanisms is essential for understanding the underlying evolutionary processes, the implementation of appropriate intervention strategies and to guarantee efficient treatment options. In the present study, 110 ß-lactam-resistant, clinical isolates of Enterobacteriaceae sampled in 2011 in one of Europe's largest hospitals, the General Hospital Vienna, were screened for the presence of 31 ß-lactamase genes. Twenty of those isolates were selected for whole genome sequencing (WGS). In addition, the number of ß-lactamase genes was estimated using biostatistical models. The carbapenemase genes blaKPC-2, blaKPC-3, and blaVIM-4 were identified in carbapenem-resistant and intermediate susceptible isolates, blaOXA-72 in an extended-spectrum ß-lactamase (ESBL)-positive one. Furthermore, the observed high prevalence of the acquired blaDHA-1 and blaCMY AmpC ß-lactamase genes (70%) in phenotypically AmpC-positive isolates is alarming due to their capability to become carbapenem-resistant upon changes in membrane permeability. The statistical analyses revealed that approximately 55% of all ß-lactamase genes present in the General Hospital Vienna were detected by this study. In summary, this work gives a very detailed picture on the disseminated ß-lactamases and other resistance genes in one of Europe's largest hospitals.


Subject(s)
Cross Infection , Enterobacteriaceae Infections/microbiology , Enterobacteriaceae/genetics , Genetic Variation , Genome, Bacterial , beta-Lactamases/genetics , Anti-Bacterial Agents/pharmacology , Austria , Carbapenems/pharmacology , Enterobacteriaceae/drug effects , Gene Frequency , Hospitals, General , Humans , Microbial Sensitivity Tests , Molecular Sequence Data , Sequence Analysis, DNA , beta-Lactam Resistance/genetics
11.
Int Orthop ; 38(8): 1597-602, 2014 Aug.
Article in English | MEDLINE | ID: mdl-24817024

ABSTRACT

PURPOSE: The purpose of our study was to evaluate and quantify the bacterial adherence to the different components of total hip prosthesis. METHODS: The bacterial load of 80 retrieved hip components from 24 patients was evaluated by counting of colony-forming units (CFU) dislodged from component surfaces using the sonication culture method. RESULTS: Micro-organisms were detected in 68 of 80 explanted components. The highest bacterial load was detected on the polyethylene liners, showing a significant difference in distribution of CFU between the liner and metal components (stem and cup). Staphylococcus epidermidis was identified as the pathogen causing the highest CFU count, especially from the polyethylene liner. CONCLUSIONS: Results of our study confirm that sonicate culture of the retrieved liners and heads, which revealed the highest bacterial loads, are reliable and sufficient for pathogen detection in the clinical diagnostic routine.


Subject(s)
Arthroplasty, Replacement, Hip/instrumentation , Bacterial Adhesion , Hip Prosthesis/microbiology , Prosthesis-Related Infections/epidemiology , Adult , Aged , Aged, 80 and over , Cell Culture Techniques/methods , Colony Count, Microbial , Female , Humans , Incidence , Male , Middle Aged , Prosthesis-Related Infections/diagnosis , Prosthesis-Related Infections/microbiology , Reproducibility of Results , Retrospective Studies , Sonication/methods , Staphylococcus epidermidis/isolation & purification
12.
Crit Care ; 17(4): R135, 2013 Jul 11.
Article in English | MEDLINE | ID: mdl-23844796

ABSTRACT

INTRODUCTION: Early initiation of appropriate antimicrobial treatment is a cornerstone in managing pneumonia. Because microbiologic processing may not be available around the clock, optimal storage of specimens is essential for accurate microbiologic identification of pathogenetic bacteria. The aim of our study was to determine the accuracy of two commonly used storage approaches for delayed processing of bronchoalveolar lavage in critically ill patients with suspected pneumonia. METHODS: This study included 132 patients with clinically suspected pneumonia at two medical intensive care units of a tertiary care hospital. Bronchoalveolar lavage samples were obtained and divided into three aliquots: one was used for immediate culture, and two, for delayed culture (DC) after storage for 24 hours at 4°C (DC4) and -80°C (DC-80), respectively. RESULTS: Of 259 bronchoalveolar lavage samples, 84 (32.4%) were positive after immediate culture with 115 relevant culture counts (≥104 colony-forming units/ml). Reduced (<104 colony-forming units/ml) or no growth of four and 57 of these isolates was observed in DC4 and DC-80, respectively. The difference between mean bias of immediate culture and DC4 (-0.035; limits of agreement, -0.977 to 0.906) and immediate culture and DC-80 (-1.832; limits of agreement, -4.914 to 1.267) was -1.788 ± 1.682 (P < 0.0001). Sensitivity and negative predictive value were 96.5% and 97.8% for DC4 and 50.4% and 75.4% for DC-80, respectively; the differences were statistically significant (P < 0.0001). CONCLUSIONS: Bronchoalveolar lavage samples can be processed for culture when stored up to 24 hours at 4°C without loss of diagnostic accuracy. Delayed culturing after storage at -80°C may not be reliable, in particular with regard to Gram-negative bacteria.


Subject(s)
Bronchoalveolar Lavage Fluid/microbiology , Intensive Care Units , Pneumonia, Bacterial/diagnosis , Pneumonia, Ventilator-Associated/diagnosis , Specimen Handling/standards , Aged , Bacterial Load , Female , Humans , Male , Middle Aged , Prospective Studies , Specimen Handling/methods , Temperature , Time Factors
13.
J Clin Microbiol ; 51(5): 1534-40, 2013 May.
Article in English | MEDLINE | ID: mdl-23486710

ABSTRACT

Time to detection (TTD) in automated blood culture systems is delayed for sensitive microorganisms in the presence of antimicrobial substances and has been associated with worse outcomes for sepsis patients on inadequate empirical therapy. While resin addition removes antimicrobial substances to various degrees from blood culture media, media formulations and the blend of resins may influence performance. The BacT/Alert 3D system (bioMérieux) was investigated using the new resin-containing medium types FA Plus (aerobic) and FN Plus (anaerobic). TTD was compared between control and test bottles containing relevant bacteria or Candida albicans, with and without defined concentrations of antimicrobials. Failure of neutralization was defined as a negative blood culture on day 3. In general, growth delay was nonlinear, concentration dependent, bottle type specific, and reciprocally associated with MICs. Substance-specific serum drug concentrations corresponding to a predefined, clinically relevant 3-h delay of TTD were calculated. Where appropriate, a time interval allowing for drug elimination below this critical level was obtained by pharmacokinetic modeling. Clarithromycin, clindamycin, gentamicin, linezolid, tigecycline, vancomycin, and fluconazole were neutralized. For ciprofloxacin and piperacillin-tazobactam, which were only incompletely neutralized in combination with the most sensitive test strains, a maximum waiting time for blood draw of 1 h was determined based on pharmacokinetics. One or more test strains did not grow in bottles containing either amoxicillin-clavulanate, cefepime, cefotaxime, meropenem, or metronidazole, and we thus recommend particular caution in timing of blood draws if patients have been pretreated with these agents.


Subject(s)
Anti-Infective Agents/pharmacology , Bacteremia/diagnosis , Bacteria/growth & development , Candida albicans/growth & development , Fungemia/diagnosis , Automation, Laboratory , Bacteremia/microbiology , Bacteria/drug effects , Candida albicans/drug effects , Culture Media , Fungemia/microbiology , Humans , Microbiological Techniques
14.
Gut ; 62(1): 34-42, 2013 Jan.
Article in English | MEDLINE | ID: mdl-22580412

ABSTRACT

OBJECTIVE: Resistance to antibiotics is the major cause of treatment failure of Helicobacter pylori infection. A study was conducted to assess prospectively the antibacterial resistance rates of H pylori in Europe and to study the link between outpatient antibiotic use and resistance levels in different countries. DESIGN: Primary antibiotic resistance rates of H pylori were determined from April 2008 to June 2009 in 18 European countries. Data on yearly and cumulative use over several years of systemic antibacterial agents in ambulatory care for the period 2001-8 were expressed in Defined Daily Doses (DDD) per 1000 inhabitants per day. The fit of models and the degree of ecological association between antibiotic use and resistance data were assessed using generalised linear mixed models. RESULTS: Of 2204 patients included, H pylori resistance rates for adults were 17.5% for clarithromycin, 14.1% for levofloxacin and 34.9% for metronidazole, and were significantly higher for clarithromycin and levofloxacin in Western/Central and Southern Europe (>20%) than in Northern European countries (<10%). Model fit improved for each additional year of antibiotic use accumulated, but the best fit was obtained for 2005. A significant association was found between outpatient quinolone use and the proportion of levofloxacin resistance (p=0.0013) and between the use of long-acting macrolides only and clarithromycin resistance (p=0.036). CONCLUSION: In many countries the high rate of clarithromycin resistance no longer allows its empirical use in standard anti-H pylori regimens. The knowledge of outpatient antibiotic consumption may provide a simple tool to predict the susceptibility of H pylori to quinolones and to macrolides and to adapt the treatment strategies.


Subject(s)
Anti-Bacterial Agents/pharmacology , Drug Resistance, Bacterial/drug effects , Drug Utilization/statistics & numerical data , Helicobacter pylori/drug effects , Adolescent , Adult , Aged , Aged, 80 and over , Ambulatory Care , Child , Child, Preschool , Clarithromycin/pharmacology , Europe , Female , Helicobacter pylori/isolation & purification , Humans , Infant , Infant, Newborn , Levofloxacin , Linear Models , Logistic Models , Male , Metronidazole/pharmacology , Microbial Sensitivity Tests , Middle Aged , Multivariate Analysis , Ofloxacin/pharmacology , Prospective Studies , Risk Factors , Surveys and Questionnaires , Young Adult
15.
Int J Artif Organs ; 35(10): 735-41, 2012 Oct.
Article in English | MEDLINE | ID: mdl-23065873

ABSTRACT

PURPOSE: The purpose of our study was to evaluate and quantify the bacterial adherence on different components of total knee prosthesis with the sonication culture method. METHODS: Explanted components of all patients with presumptive prosthetic or implant infection were treated by sonication separately in sterile containers to dislodge the adherent bacteria from the surfaces and cultured. The bacterial load of the different knee components (femur, tibia, PE-inlay and patella) was evaluated by counting of colony-forming units (CFU) dislodged from the components surfaces using the sonication culture method. RESULTS: Overall, 27 patients had positive sonication cultures of explanted total knee prostheses. Microorganisms were detected from 88 of 100 explanted components. Twenty femoral components were culture positive and 7 negative, 23 tibial components as well as 23 polyethylene (PE) platforms had positive microorganism detection from the surface. Staphylococcus epidermidis adhered to the highest number of components whereas Staphylococcus aureus yielded the highest load of CFU in the sonication cultures. Although not significant, PE-inlays and tibial components were most often affected. The highest CFU count was detected in polyethylene components. CONCLUSION: The sonication culture method is a reliable method to detect bacteria from the components. Additionally, the results demonstrate that bacterial adherence is not affecting a single component of knee prosthesis only. Thus, in septic revision surgery partial prosthetic exchange or exchange of single polyethylene components alone may be not sufficient.


Subject(s)
Bacteria/isolation & purification , Biofilms , Knee Prosthesis/adverse effects , Prosthesis-Related Infections/microbiology , Bacteria/classification , Bacteria/growth & development , Bacterial Adhesion , Bacterial Load , Biofilms/growth & development , Colony Count, Microbial , Female , Humans , Knee Prosthesis/microbiology , Male , Polyethylenes , Prosthesis Design , Sonication , Surface Properties
17.
J Clin Microbiol ; 50(1): 157-9, 2012 Jan.
Article in English | MEDLINE | ID: mdl-22075585

ABSTRACT

In this study, the PCR-based DNA strip assay GenoType BC for the identification of bacteria and the resistance genes mecA, vanA, vanB, vanC1, and vanC2/3 directly from positive BacTAlert blood culture bottles was evaluated in a multicenter study. Of a total of 511 positive blood cultures, correct identification percentages for Gram-negative bacteria, Gram-positive bacteria, and the mecA gene were 96.1%, 89.9%, and 92.9%, respectively. Results were available 4 h after growth detection.


Subject(s)
Bacteremia/diagnosis , Bacteria/isolation & purification , Blood/microbiology , DNA, Bacterial/genetics , Methicillin Resistance , Molecular Diagnostic Techniques/methods , Vancomycin Resistance , Bacteremia/microbiology , Bacteria/drug effects , Bacteria/genetics , Bacteriological Techniques/methods , DNA, Bacterial/isolation & purification , Genes, Bacterial , Humans , Polymerase Chain Reaction/methods , Sensitivity and Specificity , Time Factors
18.
J Endod ; 37(9): 1201-5, 2011 Sep.
Article in English | MEDLINE | ID: mdl-21846534

ABSTRACT

INTRODUCTION: The aims of the present study were to compare microbial populations in patients suffering from deep neck space abscesses caused by primary endodontic infections by sampling the infections with aspiration or swabbing techniques and to determine the susceptibility rates of the isolated bacteria to commonly used antibiotics. METHODS: A total of 89 patients with deep neck space abscesses caused by primary endodontic infections requiring extraoral incision and drainage under general anesthesia were included. Either aspiration or swabbing was used to sample microbial pus specimens. The culture of the microbial specimens and susceptibility testing were performed following standard procedures. RESULTS: A total of 142 strains were recovered from 76 patients. In 13 patients, no bacteria were found. The predominant bacteria observed were streptococci (36%), staphylococci (13%), Prevotella (8%), and Peptostreptococcus (6%). A statistically significant greater number of obligate anaerobes were found in the aspiration group. The majority of patients presented a mixed aerobic-anaerobic population of bacterial flora (62%). The antibiotic resistance rates for the predominant bacteria were 10% for penicillin G, 9% for amoxicillin, 0% for amoxicillin clavulanate, 24% for clindamycin, and 24% for erythromycin. CONCLUSIONS: The results of our study indicated that a greater number of anaerobes were found when sampling using the aspiration technique. Penicillin G and aminopenicillins alone are not always sufficient for the treatment of severe deep neck space abscesses; beta-lactamase inhibitor combinations are more effective. Bacteria showed significant resistant rates to clindamycin. Thus, its single use in penicillin-allergic patients has to be carefully considered.


Subject(s)
Abscess/microbiology , Dental Pulp Necrosis/complications , Dental Pulp Necrosis/microbiology , Focal Infection, Dental/microbiology , Specimen Handling/methods , Abscess/etiology , Abscess/therapy , Adolescent , Adult , Aged , Aged, 80 and over , Bacteria/isolation & purification , Child , Clindamycin/pharmacology , Colony Count, Microbial , Drainage , Drug Resistance, Microbial , Female , Humans , Male , Microbial Sensitivity Tests , Middle Aged , Neck , Penicillins/pharmacology , Prospective Studies , Suppuration/microbiology , Young Adult , beta-Lactamases/pharmacology
19.
J Pediatr Gastroenterol Nutr ; 53(1): 65-70, 2011 Jul.
Article in English | MEDLINE | ID: mdl-21694538

ABSTRACT

BACKGROUND AND OBJECTIVES: In children with clarithromycin-resistant Helicobacter pylori, clarithromycin-containing therapies often fail. The present study aimed to assess the outcome of tailored therapy upon noninvasive versus invasive H pylori susceptibility testing. PATIENTS AND METHODS: A retrospective cohort study was conducted in a pediatric outpatient clinic located in a region where H pylori clarithromycin resistance is highly prevalent. Between June 2007 and September 2009, 96 infected children (mean age 10.8 years), naïve to H pylori eradication treatment, were prescribed triple eradication therapies. These therapies were individually tailored upon susceptibility testing performed either noninvasively using stool polymerase chain reaction (stool PCR group) or invasively using endoscopy, biopsy, and culturing of gastric biopsies (gastric biopsy group). Eradication was defined by negative results upon noninvasive testing including stool PCR at least 5 weeks after the end of treatment. RESULTS: H pylori was eradicated in 43 of 55 stool PCR group versus 30 of 41 gastric biopsy group children (78.2% vs 73.2%, P = 0.63). Of those H pylori strains with pretherapeutic clarithromycin susceptibility, 78.8% were eradicated in the stool PCR group and 69.2% in the gastric biopsy group (P = 0.41) following clarithromycin-containing therapy; clarithromycin resistance was acquired by 4.1% of strains in the former group versus 12% in the latter (P = 0.33). CONCLUSIONS: Stool PCR is as effective as the invasive approach of H pylori susceptibility testing for targeting resistance-guided eradication treatments in children. Furthermore, stool PCR is a useful tool for tracking the emergence of clarithromycin resistance following eradication treatment.


Subject(s)
Drug Resistance, Bacterial , Feces/chemistry , Gastric Mucosa/microbiology , Gastritis/drug therapy , Helicobacter Infections/drug therapy , Helicobacter pylori/drug effects , Molecular Typing , Adolescent , Austria , Biopsy , Child , Clarithromycin/pharmacology , Cohort Studies , Dyspepsia/etiology , Gastric Mucosa/pathology , Gastritis/microbiology , Gastritis/pathology , Gastritis/physiopathology , Gastroscopy , Helicobacter Infections/microbiology , Helicobacter Infections/pathology , Helicobacter Infections/physiopathology , Helicobacter pylori/classification , Helicobacter pylori/isolation & purification , Humans , Male , Microbial Sensitivity Tests , Polymerase Chain Reaction , Retrospective Studies
20.
J Allergy Clin Immunol ; 128(1): 82-91.e8, 2011 Jul.
Article in English | MEDLINE | ID: mdl-21513970

ABSTRACT

BACKGROUND: Staphylococcus aureus superinfections occur in more than 90% of patients with atopic dermatitis (AD) and aggravate skin inflammation. S aureus toxins lead to tissue damage and augment T-cell-mediated skin inflammation by a superantigen effect. OBJECTIVE: To characterize IgE-reactive proteins from S aureus. METHODS: A genomic S aureus library was screened with IgE from patients with AD for DNA clones coding for IgE-reactive antigens. One was identified as fibronectin-binding protein (FBP). Recombinant FBP was expressed in Escherichia coli, purified, and tested for specific IgE reactivity in patients with AD. Its allergenic activity was studied in basophil activation experiments and T-cell cultures. The in vivo allergenic activity was investigated by sensitizing mice. RESULTS: Using IgE from patients with AD for screening of a genomic S aureus library, an IgE-reactive DNA clone was isolated that coded for FBP. Recombinant FBP was expressed in E coli and purified. It reacted specifically with IgE from patients with AD and exhibited allergenic activity in basophil degranulation assays. FBP showed specific T-cell reactivity requiring antigen presentation and induced the secretion of proinflammatory cytokines from PBMCs. Mice sensitized with FBP mounted FBP-specific IgE responses, showed FBP-specific basophil degranulation as well as FBP-specific T-cell proliferation, and mixed T(h)2/T(h)1 cytokine secretion. CONCLUSION: Evidence is provided that specific humoral and cellular immune responses to S aureus antigens dependent on antigen presentation represent a novel mechanism for S aureus-induced skin inflammation in AD. Furthermore, FBP may be used for the development of novel diagnostic and therapeutic strategies for S aureus infections.


Subject(s)
Adhesins, Bacterial/immunology , Antigen Presentation/immunology , Dermatitis, Atopic/immunology , Immunoglobulin E/immunology , Staphylococcal Infections/immunology , Staphylococcus aureus/immunology , Adhesins, Bacterial/genetics , Adolescent , Adult , Aged , Amino Acid Sequence , Animals , Base Sequence , Child , Child, Preschool , Dermatitis, Atopic/microbiology , Female , Humans , Infant , Male , Mice , Middle Aged , Molecular Sequence Data , Recombinant Proteins/immunology , Superantigens/immunology , Young Adult
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