ABSTRACT
The bovine maturation-associated sperm membrane antigen CD52-like molecule has been analysed using a mouse anti-sperm monoclonal antibody developed against bull spermatozoa. The antigen recognised by monoclonal antibody IVA-543 was detected on blood mononuclear cells (including lymphocytes and monocytes) and on a minor population of polymorphonuclear leukocytes. The bovine CD52-like molecule is secreted by the epididymal epithelium and then it is inserted into the sperm membrane during the epididymal transport in the distal part of epididymis. The CD52-like molecule was absent from spermatozoa derived from testes, and the highest proportion of IVA-543-reactive sperm was observed in the cauda epididymis (91.6%). This study has shown that the new molecule identified on bovine cells has properties analogous to those previously described for CD52 molecules in man, mouse, rat, monkey, and dog.
Subject(s)
Antibodies, Monoclonal/metabolism , Antigens, CD/chemistry , Antigens, CD/immunology , Antigens, Neoplasm/chemistry , Antigens, Neoplasm/immunology , Cattle/metabolism , Genitalia, Male/metabolism , Glycoproteins/chemistry , Glycoproteins/immunology , Sequence Homology , Animals , Antibodies, Monoclonal/immunology , Antibodies, Monoclonal/pharmacology , Antibody Specificity , Antigens, CD/isolation & purification , Antigens, CD/metabolism , Antigens, Neoplasm/isolation & purification , Antigens, Neoplasm/metabolism , Blood Cells/metabolism , CD52 Antigen , Dogs , Ejaculation , Flow Cytometry , Glycoproteins/isolation & purification , Glycoproteins/metabolism , Humans , Immunohistochemistry , Male , Mice , Mice, Inbred BALB C , Rats , Spermatozoa/metabolism , Tissue DistributionABSTRACT
Effects of ultrasound on the sex differentiation and embryonic mortality was tested on the set of 772 eggs of the Rhode Island Red and Hampshire strain chicken. In control groups, embryos were not exposed to ultrasound during incubation. In experimental groups, chicken embryos were exposed to ultrasound generated by the transducer oscillating at the frequency of 30 kHz with the power of 60 W. Embryonic mortality was significantly higher in the experimental groups exposed to ultrasound. Pathogenic effects of ultrasound were more pronounced in embryos with already developed allantochorionic blood circulation. It can be assumed that the impairment of allantoid vessels reduced in the intensity of oxidative processes in embryonic tissues. The decrease of blood pH, resulting from the carbon dioxide accumulation, set the sex differentiation in flavour of males.
Subject(s)
Embryo Loss , Sex Differentiation , Ultrasonics/adverse effects , Animals , Chick Embryo , Female , MaleABSTRACT
Cadmium was administered to 32 adult ICR mice i.p. in two single doses (0.25 and 0.5 mg CdCl2, per kg of b.w.). After 48 hours concentrations of cadmium in kidneys, liver, spleen, muscle (m. quadriceps femoris), ovaries and testes and the concentration of retinyl palmitate, retinol and beta-carotene in kidney, liver and testes were determined. Significantly higher cadmium concentration was found in liver, kidney and ovary in both experimental groups in comparison with the control group (p<0.001). In muscle, spleen and testis the cadmium level was higher, however not significantly. No significant differences in the concentration of retinyl palmitate, retinol and alpha-carotene in liver were found. Concentration of alpha-carotene in kidney and testis was significantly decreased in both groups administered with cadmium (p<0.001). Concentration of retinyl palmitate was significantly lower in testis in the group with higher cadmium level (p<0.001) and the concentration of retinol significantly decreased in kidney and testis of mice after an administration of 0.5 mg CdCl2/kg b.w.
Subject(s)
Antioxidants/metabolism , Cadmium/pharmacology , Cadmium/pharmacokinetics , Retinoids/metabolism , beta Carotene/metabolism , Animals , Anticarcinogenic Agents/pharmacokinetics , Cadmium/administration & dosage , Diterpenes , Dose-Response Relationship, Drug , Injections, Intraperitoneal , Mice , Mice, Inbred ICR , Retinyl Esters , Tissue Distribution/drug effects , Vitamin A/analogs & derivatives , Vitamin A/pharmacokineticsABSTRACT
In the work the influence of the artificial sound stimulation during incubation on the speed of the Pekin duck breed hatching as well as on their mortality during that period was observed. The set eggs were hatched in four hatcheries. The eggs of the first two hatcheries (control groups Ka and Kb) were not sound stimulated. In the other two groups the set eggs were, from the very first hour of hatching, stimulated by the "knocking" sound from an electronic sound generator (experimental groups "a" and "b"). For the stimulation, the acoustic signal with intensity of 25 dB and frequency 5.68 Hz was applied. The fastest hatching process was recorded in the groups with sound stimulation. The "a" experimental group was the fastest, the ducks were hatched after 640.75+/-10.15 hours, in the "b" experimental group the ducks were hatched after 656.50+/-1.70 hours of incubation. In both sound stimulated experimental groups (a, b) the increased embryonic mortality resulted in the decrease of the percentage of hatchability--to 68.09+/-1.77% and 76.44+/-2.68%, respectively. This is statistically evident (P<0.01) when compared with the respective control groups which reached the 88.81+/-1.86% and 89.35+/-0.81% hatchability.
Subject(s)
Acoustic Stimulation/adverse effects , Ducks/physiology , Embryo, Nonmammalian/physiology , Animals , SurvivalABSTRACT
Ten mAbs of preliminary clusters PC13 and PC27 with specificity for bovine platelets were studied by immunohistochemistry. Cryostat sections of bovine lymph node, spleen, thymus, small intestine, liver, kidney and smears of bone marrow cells were used. Five mAbs (CAPP2, IVA30, IVA125, IL-A164 and IL-A166) assigned to cluster PC13 (CD41/CD61) stained platelets and non-lymphocytic cells of various tissues. Our data confirm the presence of two specificities in PC27: three mAbs (IVA120, IVA197 and IVA198) specific for fibrinogen strongly reacted with the endothelial and reticular tissues whereas the other two mAbs Co-3D1D4 and Buf13 (WC13) were negative.
