ABSTRACT
In this study, we sequenced the complete mitochondrial genome of the Podosphaera xanthii, which is the powdery mildew diseases causative pathogen for cucurbits. The total size of the mitochondrial genome is 26,052 bp, which includes 15 coding genes, 25 tRNAs, and 2 rRNAs. The cytochrome c oxidase subunit I (COXI) used for the phylogenetic construction, which grouped this species into Hypocreomycetidae taxonomy family, which could aid the researchers to place the fungal in an appropriate taxonomy clade.
ABSTRACT
[Purpose] This study was conducted to investigate the effects of the push-up plus while using a balance ball, a sling, and a sling with a pulley in a creeping position on an unstable floor. [Subjects and Methods] The subjects were divided into three groups of 15 members each who performed the different three type methods of push-up plus (with scapulae protruding) in a random order while in a creeping posture. The muscle activity of each group in the push-up plus posture was measured using electromyography, and the measurement values were compared among the groups using one-way analysis of variance (ANOVA). [Results] The intergroup comparison revealed that the activation of the pectoralis major muscle of the balance ball exercise group was significantly decreased. In this comparison, the activity ratio for the pectoralis major muscles of the balance ball exercise group significantly decreased, and the activity ratio of their serratus anterior muscles significantly increased. From the post analysis, the differences in the activity ratios of the pectoralis major and serratus anterior muscles for the balance ball exercise group were significant when compared with those of the other groups. [Conclusion] Among the three different exercise methods in unstable situations presented in this study, the push-up plus exercise in a creeping posture using the balance ball can be recommended for the selective strengthening of the serratus anterior muscle.
ABSTRACT
BACKGROUND: We investigated heterotopic hepatocyte transplantation on biodegradable polymers as a potential treatment for end-stage liver disease. The primary problem has been insufficient engraftment of transplanted cells partly because of insufficient vascularization. Increasing vascularization through locally delivered angiogenic factors may increase angiogenesis and hepatocyte engraftment. METHODS: We studied the effect of local delivery of basic fibroblast growth factor (bFGF) on angiogenesis and hepatocyte engraftment within tissue-engineered liver constructs. Poly-l-lactic acid discs were fabricated and coated with either a mixture of saline, sucralfate, and Hydron (control group) or bFGF, sucralfate, and Hydron (bFGF group). bFGF release from polymers in vitro was tested using an ELISA. Hepatocytes were isolated from Lewis rats, seeded on control (n=9) or bFGF (n=11) polymers, and implanted into the small bowel mesentery of syngeneic animals. Specimens were harvested after 2 weeks and analyzed for hepatocyte engraftment. Microvascular density was compared between control (n=6) and bFGF groups (n=5). RESULTS: Three hundred twenty-three thousandths of a microgram of bFGF were incorporated per polymer. Greater than 99% of the bFGF was released into solution by 72 hr in vitro. Two weeks after implantation, microvascular density, as measured by capillaries per high-powered field (c/hpf), was significantly greater in the bFGF group (43.8 c/hpf), compared with the control group (30.5 c/hpf; P<0.005). Specimens from the bFGF group (mean engraftment, 61,355 microm2) showed a 2.5-fold increase in hepatocyte engraftment as compared with control (24,197 microm2; P<0.002). CONCLUSIONS: The angiogenic growth factor bFGF can be incorporated into degradable polymers used as delivery devices for hepatocyte transplantation. Implantation of these devices increases angiogenesis into the device and increases hepatocyte engraftment.
