Analysis of CDR3 Sequences from T-Cell Receptor ß in Acute Respiratory Distress Syndrome.

Acute Respiratory Distress Syndrome (ARDS) is an illness that typically develops in people who are significantly ill or have serious injuries. ARDS is characterized by fluid build-up that occurs in the alveoli. T-cells are implicated as playing a role in the modulation of the aberrant response leading to excessive tissue damage and, eventually, ARDS. Complementarity Determining Region 3 (CDR3) sequences derived from T-cells are key players in the adaptive immune response. This response is governed by an elaborate specificity for distinct molecules and the ability to recognize and vigorously respond to repeated exposures to the same molecules. Most of the diversity in T-cell receptors (TCRs) is contained in the CDR3 regions of the heterodimeric cell-surface receptors. For this study, we employed the novel technology of immune sequencing to assess lung edema fluid. Our goal was to explore the landscape of CDR3 clonal sequences found within these samples. We obtained more than 3615 CDR3 sequences across samples in the study. Our data demonstrate that: (1) CDR3 sequences from lung edema fluid exhibit distinct clonal populations, and (2) CDR3 sequences can be further characterized based on biochemical features. Analysis of these CDR3 sequences offers insight into the CDR3-driven T-cell repertoire of ARDS. These findings represent the first step towards applications of this technology with these types of biological samples in the context of ARDS.

Discrimination of different geographic varieties of Gymnema sylvestre, an anti-sweet plant used for the treatment of type 2 diabetes.

Gymnema sylvestre (Retz.) R.Br. ex Sm. (Asclepiadaceae) is a well-known Ayurvedic anti-sweet plant for the treatment of type 2 diabetes mellitus. Although it was previously proposed that G. sylvestre exhibits chemical variation based on geography, most research on G. sylvestre has used material originating from India. Morphological and anatomical descriptions, ITS1-5.8S-ITS2 DNA sequencing, and acid hydrolysis analyses showed that G. sylvestre samples from Vietnam are distinguishable from those of Indian origin and thus suggest a dissimilarity among G. sylvestre samples with different geographic distributions. An LC-MS-guided strategy targeting 3ß-glucuronide oleane-triterpenes in the Vietnamese G. sylvestre variety led to the isolation of four known compounds and nine previously undescribed compounds, named gymnemosides ND1-ND9. None of the isolated compounds were reported in the Indian sample, further supporting the geo-diversity of G. sylvestre. Three compounds, gymnemosides ND7-9, exerted significant stimulatory effects on the uptake of 2-NBDG in 3T3-L1 adipocyte cells and thus have potential as lead molecules for anti-diabetes agents.

Arrhymias in hypertensive left ventricular hypertrophy (LVH) patients by 24-hour Holter ECG

The study on 24-hour ambulatory eclectrocardiographic monitoring for 74 hypertensive patients (34 had LVH) without coronary heart disease, diabetes and 34 normal people, the groups were matched for age, sex, BMI, risk factors. Results: the frequency of supraventricular, ventricular arrhythmias and complex ventricular arrhythmias (Lown grade 3-5) in hypertensive patients, especially hypertensive left ventricular hypertrophy patients, were significantly higher than in control group. Arrhymias in hypertensive patients were associated with age, LVM. The hypertension increased the risk of supraventricular arrhythmias by 7.2 folds, ventricular arrhythmias by 7.4 folds. The hypertension with LVH increased the risk of ventricular arrhythmias by 4.04 folds, especially increased the risk of complex ventricular arrhythmias to 7.6 folds in comparison with hypertension without LVH

Study on modernisation of traditional medecine prescriptions with the aid of extraction industry and chemie of natural compounds

The study for modernization of traditional prescriptions with the help of new technology of extration and chemistry of natural compounds. With the help of new technologies, 3 traditional prescriptions – Luc Vi, Dai Bo Am, Cam Cum were modernizied. The newly prepraed product – tablets, capsules coated tablet had got pharmacopia criteria and had assured the therapentic effects, the safety thestability, the quality and the utility as the heritage of traditional medicines

Study on application of spreading cellules to investigate on skin absorption possibility of drug from TTS - nitroglycerin

The researchers built targets and way to handle skin and built quantitative method notroglucerin by high-performance liquid chromatography (HPLC) and quantitative method total nitroglucerin content in sticking-plaster Discotrin 36mg with area of 13,3cm2. The study was carried out in 6 samples and average with RSD = 0,47%, from there find out exactly nitroglycerin level on 1cm2 to evaluate permeation of nitroglycerin through skin in different temperatures ( 41oC, 37oC, 25oC). The result showed that the higher temperature, the more Nitroglycerin is permeated. The permeation of nitroglycerin through skin is defferent in each animal

To review 250 cases of large corneal transplantation in 1985-1997 year period

From the year 1985 – 1997, 250 cases of corneal transplatation were reviewed. The diameter of grafted cornea varied from 8-15mm, among them 113 cases of graft larger than 10mm. After the operation, visual acuty was improved in almost patients. In the case of internal therapy is impossible, this surgery is the only approach to save the eyeball, moreover, the optical function was restored significantly. In the case of progressive infection, which is not responded to the therapy, threatening the eyeball corneal transplantation must be indicated early.

Preliminary study on manufacture process of transdermal patch of nitroglycerin

Nitroglycerin, a vasoactive substance, has been used in the treatment of vagina for more than 100 years. To sustain the pharmaceutical effect, doses were administered every 2 hours. Recently the transdermal delivery systems have became popular. Based on eudragit E 100, we combined it with plasticizer (dibutyl sebacate/triethyl citrate), crosslinker (succinic acid), active ingredient (nitroglycerin) and further excipients (e.g. permation enhances: ethyl alcohol) to form a self adhesive matrix system for use in nitroglycerin patch. The concentration of nitroglycerin in the patch was determined by HPLC assay