ABSTRACT
Gastric cancer (GC) is the remaining concern of cancer-associated health burden. Valuable predictive and prognostic indicators support the early diagnosis and improve outcome. Immune escape and inflammation are important cancer hallmarks. The prognostic and diagnostic value of platelet-to-lymphocyte ratio (PLR) and neutrophil-to-lymphocyte ratio (NLR) was reported in some cancers. But these cheap and convenient indexes are far from clinical use. Thus, investigation the alteration of those index on GC is needed to impose the use of those indexes in clinic. The study recruited seventy-seven hospitalized patients newly diagnosed with GC and 90 healthy individuals. The clinical and preclinical data of participants were collected from Hospital Information Management system. This study were approved by the Ethical Committee, Vietnam Military Medical University. The data were analyzed on STATA version 14.0 and GraphPad Prism 8.0. The alteration of immunological system was reported by significantly higher white blood cell count, neutrophils, platelets, PLR, and NLR as well as decreased lymphocytes on GC, compared to healthy individuals. Those indexes were elevated on advanced stage GC, compared to early stage GC. Our receiver operating characteristic curve analysis showed the significant specificity and sensitivity of PLR (cutoff 135.0) and NLR (cutoff 2.0) on GC diagnosis with respective area under receiver operating characteristic curve of 84.74% and 85.17%, Pâ <â .0001. Besides, our results reported the tendency of increased PLR and NLR and short time from clinical signs to being diagnosed. PLR and NLR have significant specificity and sensitivity in diagnosis and prognosis of GC.
Subject(s)
Blood Platelets , Stomach Neoplasms , Humans , Neutrophils , Prognosis , Platelet Count , Stomach Neoplasms/diagnosis , Lymphocytes , Retrospective StudiesABSTRACT
BACKGROUND: We have followed a birth cohort from 2008 to 2009 near a dioxin-contaminated area of Da Nang, Vietnam, and investigated the effects of perinatal dioxin exposure on neurodevelopment from infancy to pre-school age. The present study aimed to investigate the effects of perinatal dioxin exposure on the learning abilities of the elementary-school children from the Da Nang birth cohort. METHODS: From 241 mother-infant pairs recruited at baseline (134 boys and 107 girls), 185 (76.8%) participated in a follow-up when the children were 8 years of age (108 boys and 77 girls). The children's perinatal dioxin exposure was estimated using the dioxin levels in their mothers' breast milk. The Colorado Learning Difficulties Questionnaire (CLDQ) was used to evaluate the children's learning difficulties. Math- and language-achievement scores were obtained using paper-based tests. Reading fluency was examined by having the children read passages in Vietnamese. RESULTS: In boys exposed to high levels of 2,3,7,8-tetrachlorodibenzodioxin (2,3,7,8-TetraCDD), CLDQ reading scores were significantly higher (worse), and language achievement scores were significantly lower. Boys exposed to high levels of 2,3,7,8-TetraCDD as well as high levels of the toxic equivalent (TEQ) of polychlorodibenzodioxins and polychlorodibenzofurans (PCDDs/Fs) had higher numbers of reading errors. Reading errors were higher and math achievement scores were lower with increasing concentrations of 1,2,3,4,7,8-HexaCDD and 1,2,3,4,6,7,8-HeptaCDD. In girls, no significant differences of any learning ability markers were found between high and low exposure groups to TEQ-PCDDs/Fs and these 3 congeners. CONCLUSIONS: Perinatal dioxin exposure may have adverse effects on the learning abilities of school children, especially boys.
Subject(s)
Dioxins/metabolism , Environmental Pollutants/metabolism , Maternal Exposure/statistics & numerical data , Milk, Human/metabolism , Prenatal Exposure Delayed Effects/epidemiology , Child , Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , Polychlorinated Dibenzodioxins/metabolism , Pregnancy , VietnamABSTRACT
BACKGROUND: Mechanisms by which anti-malarial immune responses occur are still not fully clear. Natural killer (NK) cells are thought to play a pivotal role in innate responses against Plasmodium falciparum. In this study, the suitability of NK92 cells as models for the NK mechanisms involved in the immune response against malaria was investigated. METHODS: NK92 cells were assessed for several signs of activation and cytotoxicity due to contact to parasites and were as well examined by oligonucleotide microarrays for an insight on the impact P. falciparum-infected erythrocytes have on their transcriptome. To address the parasite side of such interaction, growth inhibition assays were performed including non-NK cells as controls. RESULTS: By performing microarrays with NK92 cells, the impact of parasites on a transcriptional level was observed. The findings show that, although not evidently activated by iRBCs, NK92 cells show transcriptional signs of priming and proliferation. In addition, decreased parasitaemia was observed due to co-incubation with NK92 cells. However, such effect might not be NK-specific since irrelevant cells also affected parasite growth in vitro. CONCLUSIONS: Although NK92 cells are here shown to behave as poor models for the NK immune response against parasites, the results obtained in this study may be of use for future investigations regarding host-parasites interactions in malaria.
