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1.
Immunochemical characterization of major histocompatibility antigens in cattle.
Hoang-Xuan, M; Leveziel, H; Zilber, M T; Parodi, A L; Lévy, D.
Immunogenetics ; 15(2): 207-11, 1982.
Article in English | MEDLINE | ID: mdl-7037631

Subject(s)
Cattle/immunology , Histocompatibility Antigens/analysis , Major Histocompatibility Complex , Animals , Electrophoresis, Polyacrylamide Gel , Immunosorbent Techniques , Lymphocytes/immunology , Molecular Weight
2.
Biochemical characterization of class II bovine major histocompatibility complex antigens using cross-species reactive antibodies.
Hoang-Xuan, M; Charron, D; Zilber, M T; Levy, D.
Immunogenetics ; 15(6): 621-4, 1982.
Article in English | MEDLINE | ID: mdl-6179867

Subject(s)
Histocompatibility Antigens/isolation & purification , Animals , Antibody Specificity , Cattle , Cross Reactions , Epitopes , HLA-DR Antigens , Histocompatibility Antigens/immunology , Histocompatibility Antigens Class II/immunology , Humans , Mice , Molecular Weight , Species Specificity
3.
Typing of murine cell-surface antigens by cellular radioimmunoassay.
Levy, D; Hoang-Xuan, M; Colombani, M J; Zilber, M T; Leclerc, J C; Levy, J P.
J Immunol Methods ; 41(3): 333-41, 1981.
Article in English | MEDLINE | ID: mdl-6939754

ABSTRACT

A cellular radioimmunoassay utilizing 125I-labelled Protein A was used for detecting antigen-antibody complexes on glutaraldehyde fixed cells attached to microtiter plates. This method is rapid, sensitive and specific for revealing H-2 private and public specificities as well as Ia and Lyt antigens. As plates may be kept for months, several reactivities can be tested in one step on a large panel rendering a regular supply of animals unnecessary.


Subject(s)
Antigens, Surface/analysis , Lymphocytes/immunology , Radioimmunoassay/methods , Animals , Antigen-Antibody Reactions , Cytotoxicity, Immunologic , H-2 Antigens/analysis , Histocompatibility Antigens Class II/analysis , Mice , Staphylococcal Protein A/immunology
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