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3.
J Immunol Methods ; 41(3): 333-41, 1981.
Article in English | MEDLINE | ID: mdl-6939754

ABSTRACT

A cellular radioimmunoassay utilizing 125I-labelled Protein A was used for detecting antigen-antibody complexes on glutaraldehyde fixed cells attached to microtiter plates. This method is rapid, sensitive and specific for revealing H-2 private and public specificities as well as Ia and Lyt antigens. As plates may be kept for months, several reactivities can be tested in one step on a large panel rendering a regular supply of animals unnecessary.


Subject(s)
Antigens, Surface/analysis , Lymphocytes/immunology , Radioimmunoassay/methods , Animals , Antigen-Antibody Reactions , Cytotoxicity, Immunologic , H-2 Antigens/analysis , Histocompatibility Antigens Class II/analysis , Mice , Staphylococcal Protein A/immunology
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