1.
Chem Commun (Camb)
; 49(77): 8599-601, 2013 Oct 07.
Article
in English
| MEDLINE
| ID: mdl-23756466
ABSTRACT
Herein we report the specific labelling of the epigenetic modification 5-hydroxymethyl-cytosine along genomic DNA molecules with a fluorescent reporter molecule. Enzymatic glucosylation followed by a click chemistry reaction enables single molecule detection as well as global quantification of 5hmC in genomic DNA.