Subject(s)
Clofibrate/pharmacology , Intestine, Small/enzymology , Kidney/enzymology , Liver/enzymology , Microbodies/enzymology , Organoids/enzymology , Oxidoreductases/metabolism , Animals , Female , Intestinal Mucosa/enzymology , Mice , Mice, Obese , Microscopy, Electron , Species Specificity , Subcellular Fractions/enzymology , Subcellular Fractions/ultrastructureABSTRACT
A centrifugation binding assay has been used to demonstrate the binding of [(3)H] (±) abscisic acid to membrane-rich fractions prepared from leaves of Vicia faba L. Kinetic analysis of this binding shows evidence of saturation of binding sites with increasing concentration of ligand. Scatchard analysis of these data yields a biphasic plot possibly indicating the presence of two types of binding sites. The dissocation constant for the high affinity site has been calculated to be 3.5×10(-8) mol 1(-1).
ABSTRACT
The effects of leaf-applied (+-)-abscisic acid on the growth and dormancy of Betula pubescens Ehrh. and Alnus glutinosa Gaertn. growing under long days provide no evidence that leaf-applied abscisic acid induces or promotes the formation of resting buds in these species. Radiotracer studies show that a small percentage of the radioactivity applied as [2-(14)C]abscisic acid to the leaves accumulates in the apical region of the shoot. Of the radioactivity that was recovered from this region after 8 days, less than 10% was chromatographically similar to [2-(14)C]abscisic acid. The significance of these results with respect to the role of abscisic acid in regulating the induction of bud dormancy is discussed.
