ABSTRACT
The genotoxic effect of Rogor (an organophosphorous pesticide) at concentrations used in agriculture was studied in terms of mitotic index in onion root-tip cells, chromosome abnormalities and meiotic index in mice and lastly, lethal mutation rate in Drosophila. It was observed that the pesticide could (i) cause mitotic as well as meiotic inhibition, (ii) increase the clastogenicity rate and (iii) induce lethal mutations. The modificatory role, if any, of L-ascorbic acid (vitamin C) was studied after administering the vitamin concurrently with the pesticide. It was observed that the cytogenetic toxicity of Rogor can be appreciably minimised by vitamin C. The possible mode of antigenotoxic action of vitamin C was discussed.
Subject(s)
Antimutagenic Agents/pharmacology , Ascorbic Acid/pharmacology , Dimethoate/antagonists & inhibitors , Dimethoate/toxicity , Mutagenesis , Allium/drug effects , Animals , Drosophila melanogaster/drug effects , Drosophila melanogaster/genetics , Female , Genes, Lethal , Male , Meiosis/drug effects , Mice , Mitosis/drug effects , Mitotic Index , Mutagenicity Tests , Spermatozoa/drug effectsABSTRACT
Administration of organophosphorous pesticide Malathion and Rogor (both @ 0.2 micrograms/kg body wt/day) upto ten days was found to decrease the division rate in the primary spermatocytes of mice. The concurrent administration of vitamin B-complex (0.3 ml of 1% polybion) or ascorbic acid (0.25 ml of 1% Redoxon) with the pesticide could nullify the meiotic inhibition caused by the pesticides. The vitamins were not found to produce any significant effect on the division rate. Possible mechanism(s) behind this vitamin mediated nullification of meiotic inhibition are discussed.
Subject(s)
Ascorbic Acid/pharmacology , Dimethoate/antagonists & inhibitors , Malathion/antagonists & inhibitors , Meiosis/drug effects , Vitamin B Complex/pharmacology , Animals , Dimethoate/toxicity , Malathion/toxicity , Mice , Reproduction/drug effectsABSTRACT
Genotoxic effect of agriculture-used concentration of organophosphorous pesticide, Malathion, is decreased by the dietary concentration of sodium salt of L-ascorbic acid for parameters like mitotic-index and clastogeny in onion root-tip cells, clastogeny and meiotic-index in mice, and lethal mutation rate in Drosophila melanogaster. The vitamin itself is not genotoxic, and its concurrent administration is more effective than pretreatment with it. Possible mechanism of such vitamin C-mediated minimization of pesticide-genotoxicity is discussed.
Subject(s)
Ascorbic Acid/pharmacology , Malathion/toxicity , Animals , Bone Marrow/drug effects , Drosophila melanogaster/drug effects , Drosophila melanogaster/genetics , Female , Malathion/pharmacology , Male , Meiosis/drug effects , Mice , Mitotic Index/drug effects , Mutagens/pharmacology , Mutation , Plant Cells , Plants/drug effectsABSTRACT
Growth following renal transplantation in children and adolescents. Sixteen children who received a cadaveric renal transplant surviving 1 to 5 years, had their growth rate assessed while receiving 10 to 25 mg of prednisone every 48 hours. Six of 9 patients witha a bone age of 12 years or less grew at a "normal" rate. None of the patients whose bone age was greater than 12 years grew at a "normal" rate. Since low-dose alternate-day prednisone therapy allows for optimal growth but does not appear to compromise kidney survival, we feel such a regime should be instituted in growing children receiving a kidney transplant.
