Vaccination schedules to raise antibody concentrations against epsilon-toxin of Clostridium perfringens in ewes and their triplet lambs.

The objective of this experiment was to compare vaccination schedules for ewes and their lambs to raise antibody concentrations to epsilon-toxin of Clostridium perfringens, the causative agent of enterotoxemia. Half of 200 Finnsheep x Dorset ewes were vaccinated with C. perfringens type D toxoid vaccine 3 wk before lambing. Serum samples were obtained from 20 ewes that were to be vaccinated and 20 ewes that would remain unvaccinated before treatment and at wk 2, 1, and 0 before the start of lambing. Antibody concentrations in sera of unvaccinated ewes remained at 2 IU/mL, but they peaked in vaccinated ewes at 15 IU/mL by wk 1 before lambing. Lambs from each of the first 13 and the first 14 sets of triplets from vaccinated and unvaccinated ewes, respectively, received one of three vaccination treatments: no vaccine (control), vaccination on d 1 and 21 of age, or vaccination on d 21 and 42 of age. Antibody concentrations declined in sera of vaccinated ewes from 8.5 IU/mL immediately after lambing to 3 IU/mL 12 wk later. Vaccination of lambs did not increase sera antibody concentration. However, prepartum vaccination of ewes significantly increased lamb antibody concentrations (19 IU/mL) compared with lambs reared by unvaccinated ewes (2 IU/mL). Vaccination of ewes resulted in lambs with higher antibody concentrations until wk 10 postpartum. Concentrations declined to .6 IU/mL in all lambs at 12 wk. Because concentrations of .2 IU/mL may be protective, these results indicate that vaccination of ewes before lambing imparts passive protection in lambs to 12 wk of age, whereas vaccination of young lambs provides no added protection.

Ewe fertility in the STAR accelerated lambing system.

Effects of environmental factors such as ewe age, season of exposure, and time from lambing to exposure on fertility were evaluated using records on 1,084 Dorset ewes in the STAR accelerated lambing system. The STAR program consisted of five 30-d concurrent breeding and lambing periods per year beginning on January 1, March 15, May 27, August 8, and October 20. Fertility in the flock changed in a cyclic and predictable fashion during the year. Changes in prolificacy were less consistent but also tended to show cyclic variation. Matings that occurred within the typical breeding season (August, October, and January) were more fertile than those occurring in March and June. However, fertility also varied with the age of the ewe and the time since the ewe's last lambing. Except in June, fertility at the first postpartum mating increased as ewes aged. In March and June matings, adult ewes that had just weaned lambs were less fertile than ewes that had failed to conceive in the previous season and therefore had longer postpartum intervals. However, in October and January, ewes that had just weaned lambs were more fertile. A matrix of expected pregnancy rates, or probabilities of fertility, was constructed using a mixed GLM to describe the combined effect of season, ewe age, and time since lambing on ewe fertility in accelerated lambing.

Impact of composition manipulation on lean lamb production in the United States.

At present less than 30% of the market lambs slaughtered in the United States meet the requirements for leanness and muscling as specified in the "Certified Fresh American Lamb" program established in 1990 by the American Sheep Industry Association (ASI). Carcass composition of slaughter lambs is determined by stage of growth relative to mature size, genotype, sex, and matching dietary nutriment to nutrient requirements for lean tissue growth. On the average, current production strategies produce carcasses that contain excessive amounts of fat, impeding optimized efficiency at all levels of production. Use of large-mature-size terminal sires, feeding rumen-escape dietary protein, feeding intact males, and slaughtering at appropriate weights all improve composition of gain. Improvements of 10 to 20% in rates of gain and efficiency of nutrient use and similar reductions in feed cost can be achieved with each of these management strategies. Results from several experiments demonstrate that these effects are additive and provide a measure of the true genetic capacity for protein accretion rate in growing lambs. Adoption of these management strategies will allow lambs to be slaughtered at a younger age, which may improve meat quality and concurrently reduce the amount of nitrogen waste returned to the environment. Potential for further manipulation of composition exists through more accurately defining nutrient requirements of growing lambs and through use of metabolism modifiers. Maintaining a competitive, profitable, and sustainable sheep industry depends on continued improvement of production efficiency, preferably in systems with high reproductive rates.

Effects of exogenous somatotropin on whole-body glycemic response to insulin in young preruminant and ruminant lambs.

The purpose of this study was to determine the effects of exogenous recombinant bovine somatotropin (bST) treatment on whole-body glycemic responsiveness and sensitivity to exogenous insulin in preruminant and ruminant lambs. Twelve milk-fed (MF) and 12 ruminating (RUM) wether lambs weighing 20 +/- 0.6 kg were assigned to one of four treatment groups: MF control, MF plus bST, RUM control, and RUM plus bST. Lambs received a daily subcutaneous injection of 160 micrograms of sometribove (recombinant methionyl bST) bST/kg live weight or the equivalent volume of sterile water (control) for 10 d. The MF lambs had higher plasma insulin and nonessential fatty acids and lower acetate concentrations than RUM lambs (all P < 0.05). Plasma insulin-like growth factor concentrations were similar in both. The administration of bST raised plasma insulin-like growth factor-1 (P < 0.001) and insulin (P < 0.05) in MF and RUM lambs, but with greater effect in MF lambs (P < 0.01 and P < 0.1, respectively). Six successive dose-incremented insulin challenges (50, 100, 200, 300, 500, and 700 mU/kg body weight) were performed two per day on Days 8, 9, and 10 of treatment. Dose-response curves for absolute decline in glucose concentration from preinjection baseline to nadir were used to characterize whole-body responsiveness and sensitivity (ED50) to insulin. Somatotropin treatment increased insulin ED50 values 64 and 70% (P < 0.07) in RUM and MF lambs, respectively, suggesting that sensitivity to insulin was reduced. Insulin ED50 values were 40% higher in MF than in RUM lambs (P < 0.05). Insulin clearance rates increased with each dose increment to 300 mU/kg body weight (P = 0.001) and were 50% lower in bST-treated MF lambs than in all other treatment groups (P < 0.05). Results suggest that somatotropin modulates the insulin control of glucose homeostasis similarly in preruminant and ruminant lambs by decreasing sensitivity but not maximum responsiveness.

Abomasal casein infusion and exogenous somatotropin enhance nitrogen utilization by growing lambs.

Growing Dorset wether lambs (23 kg initial body weight) were used to determine whether the magnitude of nitrogen retention response to daily administration of exogenous somatotropin is limited by post-ruminal amino acid availability in growing ruminants. Eight lambs surgically fitted with abomasal cannulae were fed a total mixed ration of 85% of ad libitum intake. All lambs received a continuous abomasal infusion of 2 L of water or casein and twice daily subcutaneous injections of 0 or 100 micrograms recombinant bovine somatotropin (rbST)/kg body wt for 15 d per treatment in a 2 x 2 single reversal design. The casein solution was infused at a rate (4 to 5 g nitrogen/d) to achieve 25% of nitrogen intake observed with ad libitum feeding prior to initiation of treatments. Each lamb received all four treatments. Nitrogen balance was determined on d 8 to 14 of each treatment. Casein infusion increased nitrogen balance 43.4% (P less than 0.001), and rbST increased nitrogen balance 33.5% (P less than 0.001), without significant interaction (P less than 0.88). Combined effects of casein and rbST were additive, resulting in an 89% increase in nitrogen balance when compared with water plus excipient treatment. Results suggest that the quantity or composition of absorbed amino acids, or both, limit nitrogen retention by growing lambs, and that rbST increases the efficiency of utilization of absorbed amino acids for protein deposition.

Temporal pattern of skeletal muscle changes in lambs fed cimaterol.

The objectives of this study were to compare the efficacy of 3-week vs 6-week dietary administration of the beta-adrenergic agonist cimaterol on skeletal muscle growth, and to measure the changes in muscle nucleic acid and protein concentration and content to provide evidence regarding the mechanism(s) by which cimaterol stimulates muscle hypertrophy in growing ruminants. Two groups of 12 Dorset or Dorset-Finn cross ram lambs weighing 36 kg or 33 kg were assigned to treatment intervals of 3 or 6 weeks, respectively. Lambs within each weight group were randomly assigned to receive 0 or 10 ppm cimaterol in a complete mixed diet fed ad libitum. Initial live weights and treatment periods were chosen to achieve similar slaughter weights. Cimaterol increased the mass of three hind leg muscles 30% and 25% on average (both P less than .001) with 3- and 6-week administration, respectively, resulting in identical average muscle weights of treated lambs at both treatment intervals. The mean mass of these 3 muscles, expressed as a percentage of body weight, was increased 18.6% (P less than .001) at both treatment intervals. RNA concentration and content of the semitendinosus muscle were increased 24.8% (P less than .01) and 84.6% (P less than .001), respectively, after 3 weeks of treatment, but neither was significantly different from controls after 6 weeks. DNA concentration in the muscle was reduced 42% (P less than .05) with 3-week cimaterol administration, and was 25% less than controls (P greater than .05) in lambs fed cimaterol for 6 weeks. Total DNA content of the semitendinosus was unchanged at either treatment interval.(ABSTRACT TRUNCATED AT 250 WORDS)

Acute and chronic hormone and metabolite changes in lambs fed the beta-agonist, cimaterol.

The objective of this study was to determine if acute and chronic changes in circulating metabolic hormone and metabolite concentrations are associated with beta-agonist-induced nutrient repartitioning in young growing lambs. Two groups of 12 Dorset and Dorset-Finn cross ram lambs weighing 36 or 33 kg live weight were assigned to 3- or 6-week treatment intervals, respectively, to achieve similar slaughter weights. Six lambs within each treatment interval were fed ad libitum a complete mixed high-concentrate diet containing either 0 or 10 ppm cimaterol. During the first 12 hr of cimaterol administration plasma somatotropin (ST), thyroxine (T4), and triiodothyronine (T3) concentrations were not altered by treatment, but plasma insulin, glucose, non-esterified fatty acids (NEFA) and glycerol concentrations were elevated 2 hr after ingestion. These acute responses suggest direct stimulation of glycogenolysis and lipolysis by cimaterol, which is characteristic of beta-adrenergic alteration of carbohydrate and lipid metabolism. Chronic administration of cimaterol significantly decreased insulin concentrations by 36% and 52% at 3 and 6 weeks, respectively, while glucose concentrations remained unchanged. Serum IGF-I concentrations were not significantly altered by cimaterol. T4 levels were reduced 22.1% after 3 weeks of cimaterol treatment. Although plasma NEFA concentrations were chronically elevated 56% to 65% in lambs fed cimaterol, plasma glycerol concentrations remained at baseline levels. The relative changes in plasma NEFA and glycerol concentrations are consistent with a decreased rate of lipogenesis, rather than an increase in lipolysis.

Exogenous human growth hormone-releasing factor and ovine somatotropin improve growth performance and composition of gain in lambs.

The objectives of this study were 1) to compare intermittent subcutaneous administration of human growth hormone-releasing factor (hGRF) at two doses with a similar regimen of ovine somatotropin (oST) for effects on growth and composition of gain in growing lambs and 2) to determine whether increasing the dietary amino acid availability enhances response to oST or hGRF. Eighty crossbred ewe and wether lambs (25.5 kg live weight) were assigned randomly in pairs to receive four daily injections of excipient, 40 micrograms oST/kg BW, 5 micrograms hGRF/kg BW or 10 micrograms hGRF/kg BW for 42 (n = 80) or 56 (n = 40) d. Doses were adjusted weekly for BW. Mean plasma oST concentrations increased from 2.03 ng/ml prior to treatment to 20.64, 4.80 and 5.45 ng/ml with oST, 5 and 10 micrograms/kg hGRF doses, respectively. Lambs did not become refractory to hGRF. Cumulative gain increased approximately 18% with 7 wk of treatment with oST and the low dose of hGRF (both P less than .01), and feed efficiency improved 21% with oST and 18% with both doses of hGRF (both P less than .05). Carcass lipid accretion rate decreased 22% to 30% (P less than .001), and carcass protein accretion rate increased 30% to 36% (P less than .001) with hGRF and oST treatment, respectively. Addition of fishmeal to the diet at 4% to replace an equal amount of soy protein improved gain 8.5%; it improved efficiency 14.2% (P less than .05) across all treatments, and it significantly enhanced the effects of oST on feed efficiency (interaction P less than .12) and hind leg muscle weights.

Effect of season and lactation on postpartum fertility of Polypay, Dorset, St. Croix and Targhee ewes.

The objective of this study was to compare rebreeding activities of spring- vs fall-lambing Polypay, Dorset, St. Croix and Targhee ewes that either suckled their lambs for 40 d or had lambs weaned at birth. Seasonal effects of male fertility were reduced by utilizing an excess number of fertile rams in the spring. Plasma concentrations of progesterone were monitored to assess days to the first normal ovulation, days to conception and estrous vs anestrous activity. Breed, season and lactation affected the rebreeding performance. Dorset ewes had similar conception rates between spring and fall but a shorter interval from lambing to first ovulation in the fall. Polypay and Targhee ewes were the opposite; they had higher conception rates in fall than in spring matings with no seasonal influence on postpartum interval. Postpartum ewes in the fall had higher conception rates, and fewer of these ewes became anestrous or had estrous cycles of abnormal duration than of those ewes lambing in the spring. Ewes that suckled for 40 d in the spring had delayed estrous activity, but when these ewes became estrual they had higher conception rates than ewes whose lambs were weaned at birth. Lactation had no inhibitory affect on the postpartum interval of fall lambing ewes. These data suggest that the response of different breeds to various components of postpartum fertility varies with season and management of the flock.

Dietary calcium and metacarpal growth in ewes.

The effects of excess dietary calcium on bone growth were quantified in 44 ewes fed ad libitum diets that contained one of four levels of dietary calcium (0.37, 0.56, 1.13 and 1.80%) and 0.42% phosphorus. Animals were slaughtered at one of six ages, circulating concentrations of hormones and minerals were measured and bone morphometry was evaluated. None of the diets impaired normal bone growth of the metacarpal as evaluated by overall length, cortical index and growth plate width. Circulating plasma concentrations of calcium and gastrin increased as dietary calcium levels increased, but all values were within the normal range for sheep. Plasma phosphorus was unaffected by the level of dietary calcium. Diet had no affect on T3, T4 or estradiol-17 beta. Age significantly (P less than 0.05) affected the metacarpal length, growth plate width and circulating calcium and phosphorus. The metacarpal in these ewes was 95% of mature length by 172 d of age. Attainment of mature length preceded growth plate closure by slightly over 300 d. These results demonstrate that elevated dietary calcium levels do not adversely influence bone growth and that the presence of an intact growth plate does not infer that further bone growth will occur.

Cimaterol-induced muscle hypertrophy and altered endocrine status in lambs.

The objectives of this study were 1) to determine how cellular growth of skeletal muscle is altered by the repartitioning agent cimaterol and 2) to determine if cimaterol alters endocrine status in association with its repartitioning effects. Thirty Dorset wether lambs were randomly assigned to a pre-treatment baseline group or received 0 or 10 ppm cimaterol in a complete, mixed, high-concentrate diet for 7 or 12 wk. Weights of biceps femoris (BF), semimembranosus (SM) and semitendinosus (ST) muscles were 32.8, 27.1 and 31.5% greater, respectively, in treated lambs at 7 wk, and were 22 to 24% greater at 12 wk. Longissimus (LD) cross-sectional area was 26 and 32% greater at these treatment intervals. Percent type I fibers declined significantly over the course of the experiment in ST, SM and LD, and cimaterol caused a small but significant reduction in percent type I fibers in the ST at 7 and 12 wk. Muscles from lambs fed cimaterol contained 50 and 75% more fibers that exhibited negative staining for phosphorylase activity. Mean cross-sectional area of type I and type II fibers in the combined portions of the ST were 30.4 and 29.3% greater, respectively, in cimaterol-fed lambs after 12 wk, while type I and type II fiber areas in the longissimus were only 13 and 15% greater, respectively. Cimaterol-induced hypertrophy of the ST resulted in both protein and RNA content being 30 to 35% greater (P less than .01) at 7 and 12 wk, while DNA concentration was 22% less (P less than .01) at 7 wk. DNA concentration returned to normal by 12 wk. These results indicate that cimaterol elicits a rapid increase in muscle RNA and protein accretion without concurrent incorporation of satellite cell nuclei. Plasma insulin and insulin-like growth factor-1 (IGF-1) concentrations were 55 and 34% lower, respectively, in cimaterol-fed lambs. Plasma somatotropin concentration and area under the curve were 2.3 times greater (P less than .01) in lambs fed cimaterol for 6 wk, while plasma cortisol, prolactin and glucose concentration were unaffected at 6 or 12 wk. The significant changes in endocrine status may be important in the mechanism(s) of cimaterol in altering muscle accretion.

Effects of feeding monensin to lactating goats: acetyl coenzyme A carboxylase, hormone-sensitive lipase, plasma glucose, and circulating hormones.

These trials explored metabolic events associated with monensin-induced changes in milk composition. In trial 1, diets containing 0 or 33 ppm monensin sodium were fed ad libitum to separate groups of 7 mature lactating goats. In trial 2, diets containing 0 or 18 ppm monensin sodium were fed ad libitum to two groups with 5 mature (greater than 2 yr) and seven young (less than 2 yr) lactating does in each group. Blood was sampled at 1200 h and at 3 min after morning milking in both trials. Diets containing 33 ppm monensin increased serum growth hormone and plasma glucagon. Monensin (33 ppm) increased growth hormone from 13 to 60 ng/ml in samples taken 3 min after milking. Monensin (33 ppm) decreased insulin in these postmilking samples from 432 to 317 pg/ml but increased midday insulin in the samples taken between milkings from 279 to 349 pg/ml. Monensin did not affect plasma glucose or serum prolactin concentrations. Monensin fed at 18 ppm did not affect growth hormone, glucagon, adipose acetyl CoA carboxylase activity, hormone-sensitive lipase, or glucose concentrations. Young animals had higher growth hormone, glucose, and glucagon than mature does. The results indicate that effects of milk production intensity can be more important than monensin treatment on milk composition and circulating hormone concentrations.

The synthesis of glucose and ammonia by kidney tubules isolated from suckling and early-weaned lambs.

Glucose and ammonia production were examined in kidney tubules isolated from suckling and early-weaned lambs, on days 10-30 after birth, with abrupt weaning occurring at day 14. There were no differences in the rates of glucose or ammonia production for a given substrate by tubules isolated from any of the lambs, regardless of age or stage of weaning. The preferred substrates for gluconeogenesis were glycerol = lactate greater than propionate = pyruvate = fructose = proline greater than alanine greater than glutamate greater than glutamine greater than aspartate greater than glycine greater than serine, and for ammoniagenesis were glutamine much greater than alanine greater than aspartate much greater than serine greater than glycine = glutamate = proline.

Growth, intakes and metabolic responses of artificially reared lambs weaned at 14 d of age.

In two experiments, a total of 46 Finn Cross and Dorset lambs were artificially group-reared. Thirty eight were weaned abruptly at 14 d of age from a commercial milk replacer (MR) to a dry starter diet (SD). Lambs were self-fed cool (10 to 15 C) MR reconstituted to 25% dry matter (DM). The ground (2-mm screen) SD averaged 26.2% crude protein and 7.4% fat (DM basis). For both experiments, birth and weaning weights averaged 3.8 and 8.0 kg, respectively. Experiment 1 tested a strategy for encouraging postweaning DM intake. Fifteen lambs received MR reconstituted to 33% DM from d 11 to 14, and 15 lambs received standard 25% DM MR. Between d 14 and 15, intakes of DM, gross energy, crude protein and water dropped 86, 89, 85 and 64%, respectively. Lambs doubled their birth weights during the milk-feeding period and consumed 1.41 kg SD between d 14 and 25. The MR reconstitution rate did not affect weaning weight, postweaning SD or water intake, or growth check (GC, P greater than .10). Postweaning GC averaged 12.2 d and was not influenced (P greater than .10) by birth weight, sex or weaning weight. Mortality and disease rates under these conditions were negligible. Experiment 2 was designed to differentiate between the dual effects at weaning of altering the type of diet and of reducing the level of nutrient intake. Eight lambs were weaned to SD at 14 d, and eight lambs were bottle-fed isocaloric levels of MR from d 12 to 30. At weaning, plasma glucose concentration declined 1.4 mM from 6.7 mM due to fasting and an additional 1.0 mM due to the change of the type of diet (P less than .01). Plasma acetate and urea N concentrations rose steadily after d 16 in the SD-weaned lambs, but not in the MR-fed lambs (P less than .01), suggesting that the SD-weaned lambs absorbed ruminal fermentation products. These results indicate that artificially reared lambs may be routinely weaned to a dry diet at 14 d of age. The major alterations in plasma metabolites that occur within 6 to 8 d after abrupt weaning may define the period when these lambs become functional ruminants.

Effects of feeding monensin sodium to lactating goats: milk composition and ruminal volatile fatty acids.

When diets containing 33 and 18 ppm monensin sodium were fed for ad libitum intake to dairy goats, milk fat content was reduced by 15 and 5%. Milk protein content was increased 10% when 33 ppm sodium monensin was fed with diet at restricted intake. Milk yield was not affected. Both ad libitum and restricted consumption of diet containing 33 ppm monensin sodium reduced ratios of ruminal acetate:propionate. These resulted from increased propionate concentration with ad libitum consumption and from reduced acetate with restricted feeding. Diets containing 18 ppm monensin sodium resulted in slightly higher concentrations of both propionate and acetate. Monensin sodium did not reduce feed intake significantly.

Toxicologic studies with lambs fed sugar beets grown on municipal sludge-amended soil: lowered relative hemoglobin in red blood cells and mutagens in blood and excreta.

Sugar beets grown on municipal sludge-amended soil were fed to growing lambs for 66 days. The relative hemoglobin content was significantly lower (P less than 0.05) in the lambs fed the sludge-grown sugar beets. The concentration of direct-acting mutagens was significantly higher (P less than 0.05) than controls in blood and urine of the lambs fed the sludge-grown beets. Cadmium concentration was higher, but not significantly (P greater than 0.05) in the livers and kidneys of the lambs fed the sludge-grown beets as compared with controls. Significant differences between treatment groups were not observed in active or passive K+ influxes in RBC; in the activity of hepatic microsomal aniline hydroxylase in p-nitroanisole-O-demethylase, aminopyrene-N-demethylase, or arylhydrocarbon hydroxylase; in tissue ultrastructure of kidney, liver, or muscle as examined by electron microscopy; or in carcass weight, dressing percentage, quality, or yield grade.

Evaluation of a role for prolactin in growth and the photoperiod-induced growth response in sheep.

An experiment was conducted to determine whether prolactin is involved in growth or in mediating the photoperiod-induced growth response in sheep. Prolactin was manipulated by im injections of 2-Br-alpha-ergocryptine (CB154) or sc injections of ovine prolactin (oPRL) and by two light:dark regimens (16L:8D and 8L:16D). Fifty-six wether lambs (two/pen) were allotted to one of four treatments for a 9-wk growth study. Treatments were: 1) 16L:8D, placebo injections; 2) 16L:8D, CB154 injections; 3) 8L:16D, placebo injections and 4) 8L:16D, oPRL injections. Daily injections of CB154 (.1 mg/kg body weight.75), oPRL (.8 mg/kg body weight.75) or placebo were in 1 ml volume. Animals were fed ad libitum a complete mixed diet. At wk 8, plasma prolactin concentrations at 3 to 6 h postinjection were 214, 3, 90 and 228 ng/ml for treatments 1, 2, 3 and 4, respectively. Pattern of feed intake, measured at 8-h intervals for a 48-h period, was affected by photoperiod. Animals exposed to the 16L:8D photoperiod consumed 40.0, 42.4 and 17.6% of their total daily feed intake during the first 8 h of light, second 8 h of light and 8 h dark interval, respectively. Those exposed to the 8L:16D regimen consumed 55.2, 22.2 and 22.6% during their 8-h light interval, first 8 h of dark and second 8 h of dark, respectively. Both cumulative gain and feed intake were greater in 16L:8D control animals than in those animals receiving CB154.(ABSTRACT TRUNCATED AT 250 WORDS)

Influence of photoperiod and prolactin on body composition and in vitro lipid metabolism in wether lambs.

An experiment was conducted to determine the possible effects of photoperiod and prolactin on the physical and chemical composition of body weight gain. Prolactin was manipulated by im injections of 2-Br-alpha-ergocryptine (CB154) or sc injections of ovine prolactin (oPRL) and by two light:dark regimens (16L:8D and 8L:16D). The four treatments employed were: 1) 16L:8D, placebo injections; 2) 16L:8D, CB154 injections; 3) 8L:16D, placebo injections and 4) 8L:16D, oPRL injections. After a 9-wk growth study, animals were slaughtered at similar body weights over the next 2-wk period. Weight of intestines was directly related and weight of pelt inversely related to circulating concentrations of prolactin. Content of N and lipid in the carcass was not influenced by treatment. However, N content of the noncarcass components was elevated (P less than .01) in animals receiving CB154. In vitro rates of acetate incorporation (nmol X 100 mg tissue-1 X 3 h-1) were higher in subcutaneous than in perirenal adipose tissue (1,920 vs 777; P less than .001). Rates for treatments 1, 2, 3 and 4 were 1,770, 953, 1,350, respectively. The rate for treatment 1 was greater than that for treatments 2 (P less than .001) and 3 (P less than .05). There was no stimulation of lipogenesis in response to insulin added to the incubation medium. In vitro rates of glycerol release (nmol X 100 mg tissue-1 X 2 h-1) were higher in perirenal than in subcutaneous adipose (135 vs 81; P less than .001). The respective treatment rates were 120, 84, 104 and 124, with treatment 1 being greater (P less than .05) than treatment 2. Epinephrine addition elevated glycerol release (156 vs 60; P less than .001). Differences for in vitro lipid metabolism between the perirenal and subcutaneous depots were independent of differences in cell size. Overall, these data indicate that the increased body weight gain due to extended lighting was not accompanied by detrimental effects on carcass composition. Nor was there any clear relationship between circulating concentrations of prolactin and in vitro lipid metabolism.

Toxicologic studies with male sheep grazing on municipal sludge-amended soil.

Growing sheep were grazed for 152 d on grass-legume forage growing on soil that had been amended with municipal sewage sludge from Syracuse, N.Y., at 224 metric tons per hectare. Cadmium was higher, but not significantly (p greater than 0.05), in tissues of sheep fed the sludge-grown forage as compared to controls. No significant differences between the sludge or control treatments were found in weight of the complete or cauda epididymis or in percent progressive motility of cauda epididymal sperm. The sludge-treatment group had significantly larger testes (p less than 0.025) when expressed as a percentage of body weight, and higher blood uric acid values (p less than 0.05). There were no observable changes in tissue ultrastructure of liver, kidney, muscle, or testes as examined by electron microscopy in either of the treatment groups. There were no significant differences for rate of animal weight gain, carcass weight, dressing percentage, or quality or yield grade of the carcases between the treatment groups.

Toxicologic studies in growing sheep fed silage corn cultured on municipal sludge-amended acid subsoil.

Field corn was grown on subsoil, pH 5.5, that had been amended with 100 dry tons per acre (224 metric tons per hectare) of municipal sewage sludge from Syracuse, New York. The corn plants containing 3.88 ppm dry weight of cadmium were field-chopped and ensiled, and the silage was fed to growing sheep for 225 d. The sheep fed the sludge-grown corn silage showed a significantly (10 higher feed efficiency, (2) higher hepatic microsomal p-nitroanisole O-demethylase activity, and (3) higher concentrations of cadmium in liver and kidney and nickel in kidney as compared to the control animals. No significant treatment effects were observed in mutagenic responses for animal feed or feces samples. No consistent treatment effects were noted during histopathologic examination of sheep tissues.