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1.
Mol Nutr Food Res ; 51(7): 782-6, 2007 Jul.
Article in English | MEDLINE | ID: mdl-17576638

ABSTRACT

The isoflavonoids, equol, formononetin, daidzein, genistein, biochanin A, and O-demethylangolensin (O-DMA), were analyzed from commercial cartons of skimmed Finnish milk by HPLC-diode array detector (DAD)-FL. We found 411 +/- 65 ng/mL of equol and traces of formononetin and daidzein in organic skimmed milk whereas conventionally produced milk contained 62 +/- 16 ng/mL of equol and no formononetin or daidzein.


Subject(s)
Isoflavones/analysis , Milk/chemistry , Animals , Cattle , Chromatography, High Pressure Liquid , Equol , Finland , Food, Organic/analysis
2.
Biochim Biophys Acta ; 1738(1-3): 115-20, 2005 Dec 30.
Article in English | MEDLINE | ID: mdl-16326136

ABSTRACT

Soy-derived isoflavone phytoestrogens, such as genistein (4',5,7-trihydroxyisoflavone), have been shown to protect low-density lipoprotein from oxidation. In addition, human plasma was previously shown to be capable of converting genistein into lipophilic fatty acid esters that accumulate in lipoproteins in vitro. We developed a method for the quantitation of genistein fatty acid esters in plasma. Furthermore, the method was utilized to measure genistein ester concentrations in monkey plasma following administration of genistein or genistein 4',7-O-dioleate. After extraction from plasma, genistein fatty acid esters were separated from unesterified genistein by Sephadex LH-20 column chromatography. The genistein ester fraction was hydrolyzed by saponification and purified by a second chromatography on Sephadex LH-20. The hydrolyzed genistein esters were measured by time-resolved fluoroimmunoassay. Adult female rhesus monkeys (n=10) received a subcutaneous injection of genistein (24 mg, n=2) or genistein 4',7-O-dioleate (71 mg, n=3) or an oral dose of genistein (24 mg, n=2) or genistein 4',7-O-dioleate (71 mg, n=3). Plasma was collected at 4, 8, and 24 h post-dosing. Following subcutaneous administration of genistein 4',7-O-dioleate, the plasma concentrations of genistein esters became elevated in two out of three monkeys with 8-h values exceeding 7.5 nmol/L and 24-h values above 12 nmol/L. Other treatments resulted in lower plasma values ranging between 2.7 and 6.1 nmol/L. The lower limit of detection for the method was 1.44 nmol/L. Subcutaneously administered genistein 4',7-O-dioleate was also converted to water-soluble conjugates, but oral administration did not elevate plasma genistein fatty acid ester levels. The results suggest that it may be possible to introduce intact genistein ester molecules into plasma by parenteral but not oral administration.


Subject(s)
Blood Chemical Analysis/methods , Esters/blood , Fatty Acids/blood , Genistein/analogs & derivatives , Genistein/blood , Genistein/pharmacology , Oleic Acids/blood , Administration, Oral , Animals , Esters/metabolism , Fatty Acids/metabolism , Female , Fluoroimmunoassay/methods , Genistein/administration & dosage , Injections, Subcutaneous , Macaca mulatta , Sensitivity and Specificity
3.
J Steroid Biochem Mol Biol ; 87(4-5): 285-99, 2003 Dec.
Article in English | MEDLINE | ID: mdl-14698210

ABSTRACT

Epidemiological studies have associated high soy intake with a lowered risk for certain hormone-dependent diseases. Soy and soy foods are rich sources of isoflavones, which have been shown to possess several biological activities. In this study, the metabolism of soy isoflavones daidzein, genistein and glycitein was investigated in human subjects. The aim was to find and identify urinary phase I metabolites of isoflavones, which have an intact isoflavonoid skeleton, and which might possess some bioactivity. Six volunteers included three soy bars per day into their normal western diet for a 2-week period. Daily urine samples were collected before, and after the supplementation period. Urine samples were hydrolyzed with Helix pomatia, extracted with diethyl ether, purified with Sephadex LH-20 chromatography, and analyzed as trimethylsilyl derivatives using gas chromatography-mass spectrometry (GC-MS). The structures of the isoflavone metabolites were identified using authentic reference compounds. The metabolites, for which authentic reference compounds were not available, were identified by the interpretation of mass spectra. Several new isoflavone metabolites were identified, and the presence of previously reported metabolites confirmed. The metabolic pathways of daidzein, genistein and glycitein are presented on the basis of the identification of the metabolites in human urine after soy supplementation.


Subject(s)
Genistein/urine , Glycine max/chemistry , Isoflavones/urine , Adult , Chromatography, Gas , Dietary Supplements , Female , Genistein/chemistry , Humans , Isoflavones/chemistry , Male , Reference Standards , Spectrometry, Mass, Electrospray Ionization
4.
Br J Nutr ; 89 Suppl 1: S5-18, 2003 Jun.
Article in English | MEDLINE | ID: mdl-12725650

ABSTRACT

A review covering different methods for the analysis of phyto-oestrogens in biological matrices is presented. Sample pretreatment and analysis of isoflavonoids and lignans by HPLC and GC with various detection methods are discussed. The immunoassay method is also briefly presented.


Subject(s)
Estrogens, Non-Steroidal/analysis , Chromatography, Gas/methods , Chromatography, High Pressure Liquid/methods , Humans , Immunoassay/methods , Isoflavones/analysis , Lignans/analysis , Phytoestrogens , Plant Preparations , Plants/chemistry
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