ABSTRACT
Saksenaea is a monotypic genus belonging to the order Mucorales and capable of producing severe human infections. Through a polyphasic study based on analysis of the sequences of the internal transcribed spacer (ITS) region, domains D1 and D2 of the 28S rRNA gene, and the elongation factor 1α (EF-1α) gene, as well as by evaluation of relevant morphological and physiological characteristics of a set of clinical and environmental strains, we have demonstrated that Saksenaea vasiformis is a complex of species. We propose as new species Saksenaea oblongispora, characterized by oblong sporangiospores and unable to grow at 42°C, and Saksenaea erythrospora, characterized by large sporangiophores and sporangia and by ellipsoid sporangiospores, biconcave in the lateral view. Itraconazole, posaconazole, and terbinafine were active against all isolates included in the study, while amphotericin B, voriconazole, and the echinocandins showed low activity.
Subject(s)
Mucorales/classification , Mucorales/genetics , Antifungal Agents/pharmacology , DNA, Fungal/chemistry , DNA, Fungal/genetics , DNA, Ribosomal Spacer/chemistry , DNA, Ribosomal Spacer/genetics , Environmental Microbiology , Fungal Proteins/genetics , Humans , Itraconazole/pharmacology , Microbial Sensitivity Tests , Molecular Sequence Data , Mucorales/cytology , Mucorales/physiology , Mucormycosis/microbiology , Mycological Typing Techniques , Naphthalenes/pharmacology , Peptide Elongation Factor 1/genetics , Phylogeny , Sequence Analysis, DNA , Spores, Fungal/cytology , Spores, Fungal/growth & development , Temperature , Terbinafine , Triazoles/pharmacologyABSTRACT
The in vitro activities of caspofungin and micafungin against 1,038 yeast isolates have been determined. The caspofungin and micafungin MICs were lower for Candida albicans, Candida glabrata, and Candida tropicalis than for Candida parapsilosis, Candida guilliermondii, and Candida krusei. A clear correlation was seen between the MICs for the two drugs.
