Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 7 de 7
Filter
Add more filters










Database
Language
Publication year range
1.
Med Mycol ; 57(7): 800-806, 2019 Oct 01.
Article in English | MEDLINE | ID: mdl-30649403

ABSTRACT

The diagnosis of coccidioidomycosis (CM) in dogs is typically based on clinical presentation, serology, and (less frequently) spherule identification. Agar gel immunodiffusion (AGID) is the most commonly employed serological method, but AGID is slow (requiring up to a week for titer). A Coccidioides antigen enzyme immunoassay (EIA) is also available; however, sensitivity is low in CM dogs. An antibody EIA was developed to detect canine immunoglobulin G (IgG) reacting to Coccidioides antigens. Serum was evaluated from dogs with pathology proven CM and/or AGID positive CM, as well as dogs with histoplasmosis, blastomycosis, non-fungal infections, or healthy dogs. A standard curve was used to convert optical density (OD) values into EIA units (EU). Serum and urine samples from CM dogs were also tested in the antigen EIA. Sensitivity and specificity for IgG were 89.2% and 97.2%, respectively, upon evaluation of dogs with proven or probable CM and control dogs. Cross-reactivity was observed in 7.7% and in 6.4% of dogs with histoplasmosis or blastomycosis, respectively. The antigen EIA alone was insensitive (33.8%). Combined IgG and antigen testing increased sensitivity to 93.2%, as three dogs were IgG-negative but had detectable serum or urine antigen. In 22 dogs with proven CM, sensitivity was statistically similar for antibody EIA and AGID (86% and 73%; P = .487). The MiraVista® canine Coccidioides antibody IgG EIA may aid in the diagnosis of CM by improving turnaround time with comparable sensitivity to AGID. Serial or concurrent testing by antibody and antigen EIAs may be beneficial when screening dogs for CM.


Subject(s)
Antibodies, Fungal/blood , Coccidioidomycosis/veterinary , Dog Diseases/diagnosis , Immunoenzyme Techniques/methods , Immunoglobulin G/blood , Animals , Antigens, Fungal/immunology , Blastomycosis , Coccidioides/immunology , Coccidioidomycosis/diagnosis , Cross Reactions , Dog Diseases/immunology , Dog Diseases/microbiology , Dogs , Histoplasmosis , Immunoglobulin M , Sensitivity and Specificity
2.
Infect Immun ; 82(10): 4414-25, 2014 Oct.
Article in English | MEDLINE | ID: mdl-25114108

ABSTRACT

The yeast phase of Histoplasma capsulatum is the virulent form of this thermally dimorphic fungal pathogen. Among the secreted proteome of Histoplasma, culture filtrate protein 4 (Cfp4) is a heavily glycosylated factor produced abundantly and specifically by Histoplasma yeast cells, suggesting its role in pathogenesis. We have generated three monoclonal antibodies as tools for characterization and detection of Cfp4 and determined the epitope each recognizes. Through site-directed mutagenesis of Cfp4, we identified three asparagines that function as the principal sites of N-linked glycan modification. To test the function of Cfp4 in Histoplasma pathogenesis, we generated Cfp4-deficient strains by insertional mutagenesis and by RNA interference. Cfp4-deficient strains are not attenuated in virulence in human macrophages or during lung infection in a murine model of histoplasmosis. Coinfection of differentially marked Cfp4-producing and Cfp4-deficient strains demonstrates that production of Cfp4 does not confer a fitness advantage to Histoplasma yeasts during murine lung infection. Despite no apparent role in acute virulence in mice, secretion of the Cfp4 glycoprotein by yeast cells is consistent across clinical and laboratory isolates of the North American type 1 and type 2 phylogenetic groups as well as a strain from Panama. In addition, human immune sera recognize the Histoplasma Cfp4 protein, confirming Cfp4 production during infection of human hosts. These results suggest the potential utility of Cfp4 as a diagnostic exoantigen for histoplasmosis.


Subject(s)
Fungal Proteins/immunology , Histoplasma/immunology , Protein Processing, Post-Translational , Animals , Antibodies, Fungal/immunology , Antibodies, Monoclonal/immunology , Cells, Cultured , DNA Mutational Analysis , Disease Models, Animal , Epitope Mapping , Fungal Proteins/genetics , Fungal Proteins/metabolism , Gene Knockdown Techniques , Gene Knockout Techniques , Glycosylation , Histoplasma/genetics , Histoplasma/metabolism , Histoplasmosis/microbiology , Histoplasmosis/pathology , Humans , Macrophages/immunology , Macrophages/microbiology , Mice , Mice, Inbred C57BL , Mutagenesis, Insertional , RNA Interference
3.
Infect Immun ; 81(7): 2334-46, 2013 Jul.
Article in English | MEDLINE | ID: mdl-23589579

ABSTRACT

Histoplasma capsulatum is a respiratory pathogen that infects phagocytic cells. The mechanisms allowing Histoplasma to overcome toxic reactive oxygen molecules produced by the innate immune system are an integral part of Histoplasma's ability to survive during infection. To probe the contribution of Histoplasma catalases in oxidative stress defense, we created and analyzed the virulence defects of mutants lacking CatB and CatP, which are responsible for extracellular and intracellular catalase activities, respectively. Both CatB and CatP protected Histoplasma from peroxide challenge in vitro and from antimicrobial reactive oxygen produced by human neutrophils and activated macrophages. Optimal protection required both catalases, as the survival of a double mutant lacking both CatB and CatP was lower than that of single-catalase-deficient cells. Although CatB contributed to reactive oxygen species defenses in vitro, CatB was dispensable for lung infection and extrapulmonary dissemination in vivo. Loss of CatB from a strain also lacking superoxide dismutase (Sod3) did not further reduce the survival of Histoplasma yeasts. Nevertheless, some catalase function was required for pathogenesis since simultaneous loss of both CatB and CatP attenuated Histoplasma virulence in vivo. These results demonstrate that Histoplasma's dual catalases comprise a system that enables Histoplasma to efficiently overcome the reactive oxygen produced by the innate immune system.


Subject(s)
Catalase/metabolism , Gene Expression Regulation, Fungal , Histoplasma/pathogenicity , Neutrophils/metabolism , Oxidative Stress , Animals , Catalase/genetics , Enzyme Activation , Fungal Proteins/genetics , Fungal Proteins/metabolism , Gene Deletion , Genes, Fungal , Histoplasma/enzymology , Histoplasma/genetics , Histoplasmosis/microbiology , Histoplasmosis/pathology , Humans , Hydrogen Peroxide/pharmacology , Male , Mice , Mice, Inbred C57BL , Neutrophils/microbiology , RNA, Fungal/genetics , Reactive Oxygen Species/metabolism , Superoxide Dismutase/genetics , Superoxide Dismutase/metabolism , Virulence
4.
PLoS Pathog ; 8(5): e1002713, 2012.
Article in English | MEDLINE | ID: mdl-22615571

ABSTRACT

In order to establish infections within the mammalian host, pathogens must protect themselves against toxic reactive oxygen species produced by phagocytes of the immune system. The fungal pathogen Histoplasma capsulatum infects both neutrophils and macrophages but the mechanisms enabling Histoplasma yeasts to survive in these phagocytes have not been fully elucidated. We show that Histoplasma yeasts produce a superoxide dismutase (Sod3) and direct it to the extracellular environment via N-terminal and C-terminal signals which promote its secretion and association with the yeast cell surface. This localization permits Sod3 to protect yeasts specifically from exogenous superoxide whereas amelioration of endogenous reactive oxygen depends on intracellular dismutases such as Sod1. While infection of resting macrophages by Histoplasma does not stimulate the phagocyte oxidative burst, interaction with polymorphonuclear leukocytes (PMNs) and cytokine-activated macrophages triggers production of reactive oxygen species (ROS). Histoplasma yeasts producing Sod3 survive co-incubation with these phagocytes but yeasts lacking Sod3 are rapidly eliminated through oxidative killing similar to the effect of phagocytes on Candida albicans yeasts. The protection provided by Sod3 against host-derived ROS extends in vivo. Without Sod3, Histoplasma yeasts are attenuated in their ability to establish respiratory infections and are rapidly cleared with the onset of adaptive immunity. The virulence of Sod3-deficient yeasts is restored in murine hosts unable to produce superoxide due to loss of the NADPH-oxidase function. These results demonstrate that phagocyte-produced ROS contributes to the immune response to Histoplasma and that Sod3 facilitates Histoplasma pathogenesis by detoxifying host-derived reactive oxygen thereby enabling Histoplasma survival.


Subject(s)
Histoplasma/enzymology , Histoplasma/pathogenicity , Histoplasmosis/immunology , Oxidative Stress , Reactive Oxygen Species/metabolism , Superoxide Dismutase/metabolism , Animals , Histoplasmosis/metabolism , Histoplasmosis/microbiology , Macrophages/immunology , Macrophages/microbiology , Mice , Mice, Inbred C57BL , Neutrophils/immunology , Neutrophils/microbiology , Phagocytosis , RNA Interference , RNA, Small Interfering , Superoxide Dismutase/biosynthesis
5.
J Proteome Res ; 10(4): 1929-43, 2011 Apr 01.
Article in English | MEDLINE | ID: mdl-21291285

ABSTRACT

The dimorphic fungal pathogen Histoplasma capsulatum causes respiratory and systemic disease. Within the mammalian host, pathogenic Histoplasma yeast infect, replicate within, and ultimately kill host phagocytes. Surprisingly, few factors have been identified that contribute to Histoplasma virulence. To address this deficiency, we have defined the constituents of the extracellular proteome using LC-MS/MS analysis of the proteins in pathogenic-phase culture filtrates of Histoplasma. In addition to secreted Cbp1, the extracellular proteome of pathogenic Histoplasma yeast consists of 33 deduced proteins. The proteins include glycanases, extracellular enzymes related to oxidative stress defense, dehydrogenase enzymes, chaperone-like factors, and five novel culture filtrate proteins (Cfp's). For independent verification of proteomics-derived identities, we employed RNA interference (RNAi)-based depletion of candidate factors and showed loss of specific proteins from the cell-free culture filtrate. Quantitative RT-PCR revealed the expression of 10 of the extracellular factors was particularly enriched in pathogenic yeast cells as compared to nonpathogenic Histoplasma mycelia, suggesting that these proteins are linked to Histoplasma pathogenesis. In addition, Histoplasma yeast express these factors within macrophages and during infection of murine lungs. As extracellular proteins are positioned at the interface between host and pathogen, the definition of the pathogenic-phase extracellular proteome provides a foundation for the molecular dissection of how Histoplasma alters the host-pathogen interaction to its advantage.


Subject(s)
Fungal Proteins/analysis , Histoplasma/chemistry , Histoplasma/pathogenicity , Proteome/analysis , Animals , Chromatography, Liquid/methods , Fungal Proteins/genetics , Histoplasma/genetics , Host-Pathogen Interactions , RNA Interference , Tandem Mass Spectrometry/methods
6.
RNA ; 15(2): 255-65, 2009 Feb.
Article in English | MEDLINE | ID: mdl-19095617

ABSTRACT

The exit (E) site has been implicated in several ribosomal activities, including translocation, decoding, and maintenance of the translational reading frame. Here, we target the 30S subunit E site by introducing a deletion in rpsG that truncates the beta-hairpin of ribosomal protein S7. This mutation (S7DeltaR77-Y84) increases both -1 and +1 frameshifting but does not increase miscoding, providing evidence that the 30S E site plays a specific role in frame maintenance. Mutation S7DeltaR77-Y84 also stimulates +1 programmed frameshifting during prfB'-lacZ translation in many synthetic contexts. However, no effect is seen when the E codon of the frameshift site corresponds to those found in nature, suggesting that E-tRNA release does not normally limit the rate of prfB frameshifting. Ribosomes containing S7DeltaR77-Y84 exhibit an elevated rate of spontaneous reverse translocation and an increased K (1/2) for E-tRNA. These effects are of similar magnitude, suggesting that both result from destabilization of E-tRNA. Finally, this mutation of the 30S E site does not inhibit EF-G-dependent translocation, consistent with a primary role for the 50S E site in the mechanism.


Subject(s)
Escherichia coli/metabolism , Frameshifting, Ribosomal/genetics , Gene Expression Regulation, Bacterial , Ribosome Subunits, Small, Bacterial/physiology , Amino Acid Sequence , Escherichia coli/genetics , Molecular Sequence Data , Mutation , Open Reading Frames , Peptide Elongation Factor G/metabolism , Protein Structure, Secondary , Protein Transport , Ribosome Subunits, Small, Bacterial/chemistry , Ribosome Subunits, Small, Bacterial/genetics
7.
Curr Opin Microbiol ; 11(4): 318-24, 2008 Aug.
Article in English | MEDLINE | ID: mdl-18573684

ABSTRACT

The dimorphism of Histoplasma reflects a developmental switch in morphology and lifestyle that is necessary for virulence. The dimorphism regulating kinase DRK1 and the Histoplasma WOR1 homolog RYP1 mediate the thermally induced transition to the pathogenic yeast-phase program. The genes expressed as part of this regulon influence the host-pathogen interaction to favor Histoplasma virulence. While surface localized HSP60 supports yeast attachment to host macrophages, yeast alpha-glucan polysaccharides conceal immunostimulatory cell wall beta-glucans from detection by macrophage receptors. Intramacrophage growth of yeast cells is facilitated by CBP a secreted, protease-resistant calcium-binding protein tailored to function within the phagolysosomal environment. In some Histoplasma strains, YPS3 promotes dissemination of yeast from pulmonary infection sites. The Histoplasma yeast-phase program includes additional cell surface and extracellular molecules that potentially function in further aspects of Histoplasma virulence.


Subject(s)
Histoplasma/pathogenicity , Histoplasmosis/microbiology , Gene Expression Regulation, Fungal , Histoplasma/growth & development , Histoplasma/immunology , Histoplasmosis/immunology , Virulence
SELECTION OF CITATIONS
SEARCH DETAIL
...