ABSTRACT
A heterogeneous group of 10 male and 15 female judo players are utilized in this study. The subjects complete a standardized maximal treadmill exercise test. Urine samples are collected at the pre- and postexercise stages. The urine steroids are measured using a gas chromatography-mass spectrometry instrument. In rest and after exercise, significantly higher testosterone and epitestosterone concentrations in males (p < 0.01) are found. The etiocholanolone-dehydroepiandrosterone (DHEA) ratio is significantly lower in males than females (p < 0.05). In both males and females, etiocholanolone concentration significantly decreases with the effect of exercise (p < 0.05). 11-OH etiocholanolone concentration also significantly decreases, but only in females (p < 0.05). Positive correlation is found between the changes of the etiocholanolone and epitestosterone concentration caused by exercise.
Subject(s)
Androstanols/urine , Exercise , Martial Arts , Female , Gas Chromatography-Mass Spectrometry , Humans , MaleSubject(s)
Computer Simulation , Selegiline/metabolism , Animals , Female , Gas Chromatography-Mass Spectrometry , Humans , Male , Methamphetamine/metabolism , Rats , Selegiline/chemistryABSTRACT
The metabolism and urinary elimination of both (-)-deprenyl and (+)-deprenyl have been studied. Gas-chromatographic analysis with mass specific detection indicated that the metabolism of (-)-deprenyl results in a large excess of methamphetamine compared to amphetamine, while the metabolism of (+)-deprenyl gave nearly equal amounts of amphetamine and methamphetamine. A novel deprenyl metabolite, phenylacetone, was also identified in our studies.
Subject(s)
Gas Chromatography-Mass Spectrometry/methods , Monoamine Oxidase Inhibitors/pharmacokinetics , Neuroprotective Agents/pharmacokinetics , Selegiline/pharmacokinetics , Amphetamine/urine , Humans , Male , Methamphetamine/urine , Monoamine Oxidase Inhibitors/chemistry , Monoamine Oxidase Inhibitors/urine , Neuroprotective Agents/chemistry , Neuroprotective Agents/urine , Selegiline/chemistry , Selegiline/urine , StereoisomerismABSTRACT
(+)-Deprenyl metabolites in rat's urine, such as nordeprenyl. methamphetamine amphetamine and p-hydroxy. methamphetamine were identified by HPLC-MS. After oral administration of 10 mg of pure (-)- and (+)-deprenyl to human volunteers, their urine was analyzed by gas chromatography. The concentration of methamphetamine was found to be overwhelming in the case of the (-)-isomer, while amphetamine and methamphetamine were excreted in equal amounts when (+)-deprenyl was administered. The metabolic processes of deprenyl resulted in metabolites possessing different lipophilicity, as it has been shown by planar displacement chromatography.
Subject(s)
Chromatography, Gas/methods , Chromatography, High Pressure Liquid/methods , Chromatography, Thin Layer/methods , Mass Spectrometry/methods , Monoamine Oxidase Inhibitors/urine , Selegiline/urine , Animals , Female , Humans , Male , Monoamine Oxidase Inhibitors/administration & dosage , Monoamine Oxidase Inhibitors/metabolism , Rats , Rats, Wistar , Selegiline/administration & dosage , Selegiline/metabolismABSTRACT
The secondary structure of a synthetic amyloid fragment des [Ala21,30]A42 was studied by circular dichroism and Fourier transformed infrared spectroscopy. Measurements were performed in trifluoroethanol/water and octyl beta-D-glucopyranoside solutions. The spectra of the peptide in trifluoroethanol indicate a high percentage of alpha-helical structure. However, in octyl glucoside, at and above the critical micelle concentration, the peptide adopts a beta-sheet conformation. Secondary structure analysis yields a predominant (> 70%) beta-sheet content. Our data suggest that the peptide backbone or polar side groups of des[Ala21,30]A42 interact with the sugar-coated surface of micelles, which promotes an alpha to beta conformational transition.