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Methods Mol Biol ; 2764: 61-73, 2024.
Article in English | MEDLINE | ID: mdl-38393589

ABSTRACT

Biomimetic semi-synthetic hydrogels formed from a combination of star-shaped poly(ethylene glycol) (starPEG) and the glycosaminoglycan, heparin, allows for the three-dimensional (3D) culture of various cells and tissues. In this chapter, we describe methods for the use of starPEG-heparin hydrogels to cultivate primary and immortalized human acute myeloid leukemia (AML) cells. The resulting 3D culture models allow for the study of AML development and response to chemotherapeutic agents.


Subject(s)
Heparin , Leukemia, Myeloid, Acute , Humans , Hydrogels , Glycosaminoglycans , Leukemia, Myeloid, Acute/drug therapy , Polyethylene Glycols
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