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1.
J Cell Biol ; 128(6): 1229-41, 1995 Mar.
Article in English | MEDLINE | ID: mdl-7534767

ABSTRACT

CD31 is a member of the immunoglobulin superfamily consisting of six Ig-related domains. It is constitutively expressed by platelets, monocytes, and some lymphocytes, but at tenfold higher levels on vascular endothelial cells. CD31 has both homotypic and heterotypic adhesive properties. We have mapped the homotypic binding sites using a deletion series of CD31-Fc chimeras and a panel of anti-CD31 monoclonal antibodies. An extensive surface of CD31 is involved in homotypic binding with domains 2 and 3 and domains 5 and 6 playing key roles. A model consistent with the experimental data is that CD31 on one cell binds to CD31 on an apposing cell in an antiparallel interdigitating mode requiring full alignment of the six domains of each molecule. In addition to establishing intercellular homotypic contacts. CD31 binding leads to augmented adhesion via beta 1 integrins. The positive cooperation between CD31 and beta 1 integrins can occur in heterologous primate cells (COS cells). The interaction is specific to both CD31 and beta 1 integrins. Neither intercellular adhesion molecule-1 (ICAM-1)/leukocyte function-associated antigen-1 (LCAM-1) nor neural cell adhesion molecule (NCAM)/NCAM adhesion leads to recruitment of beta 1 integrin adhesion pathways. Establishment of CD31 contacts have effects on the growth and morphology of endothelial cells. CD31(D1-D6)Fc inhibits the growth of endothelial cells in culture. In addition, papain fragments of anti-CD31 antibodies (Fab fragments) disrupt interendothelial contact formation and monolayer integrity when intercellular contacts are being formed. The same reagents are without effect once these contacts have been established, suggesting that CD31-CD31 interactions are critically important only in the initial phases of intercellular adhesion.


Subject(s)
Antigens, Differentiation, Myelomonocytic/chemistry , Antigens, Differentiation, Myelomonocytic/metabolism , Cell Adhesion Molecules/chemistry , Cell Adhesion Molecules/metabolism , Integrins/metabolism , Binding Sites , Cell Adhesion , Cell Communication , Endothelium, Vascular/cytology , Endothelium, Vascular/metabolism , Humans , Integrin beta1 , Platelet Endothelial Cell Adhesion Molecule-1 , Recombinant Fusion Proteins/metabolism
2.
Eur J Immunol ; 25(2): 459-65, 1995 Feb.
Article in English | MEDLINE | ID: mdl-7875209

ABSTRACT

Intercellular adhesion molecule-3 (ICAM-3, CD50), a member of the immunoglobulin gene superfamily, is a major ligand for the lymphocyte function-associated antigen 1 (LFA-1, CD18/CD11a) in the resting immune system and plays a role as a signaling and costimulatory molecule on T lymphocytes. In this study we have generated a large panel of anti-ICAM-3 monoclonal antibodies (mAb) and show that the biological effects of these antibodies are critically dependent on the epitope recognized. By using an adhesion assay employing COS cells expressing LFA-1 binding to recombinant chimeric ICAM-3-Fc proteins (which overcomes the confounding effects of interleukocyte LFA-1/ICAM binding events), we have been able to examine the effects of these antibodies in blocking LFA-1/ICAM-3 adhesion. Our data suggests that only a small minority of ICAM-3 mAb, recognizing a distinct epitope, are able to mimic the effects of LFA-1 binding to ICAM-3. Moreover these antibodies are functionally distinct as defined by their costimulatory activity and ability to elicit interleukin-2 production and cell proliferation in T lymphocytes.


Subject(s)
Antibodies, Monoclonal/immunology , Antigens, CD , Antigens, Differentiation , Cell Adhesion Molecules/immunology , Epitope Mapping , Animals , Antibodies, Monoclonal/physiology , Cell Adhesion Molecules/physiology , Cell Aggregation , Cell Line , Humans , Lymphocyte Activation , Lymphocyte Function-Associated Antigen-1/physiology , Mice , Mice, Inbred BALB C
3.
J Biol Chem ; 270(2): 877-84, 1995 Jan 13.
Article in English | MEDLINE | ID: mdl-7822326

ABSTRACT

Intercellular adhesion molecule 3 (ICAM-3, CD50) is a member of the immunoglobulin superfamily and is a constitutively expressed ligand for the leukocyte integrin LFA-1 (CD11a/CD18). ICAM-3 is expressed at high levels by all resting leukocyte populations and antigen presenting cells and is a major ligand for LFA-1 in the resting immune system. ICAM-3 is a signal transducer and may play a key role in initiating immune responses. Mutant ICAM-3 Fc-chimeric proteins were quantitatively analyzed for their ability to bind COS cells expressing human LFA-1. The LFA-1-binding site on ICAM-3 is located in the N-terminal 2 Ig domains. Domains 3-5 do not significantly contribute to adhesion. The binding site has been further resolved by rational targeting of 14 point mutations throughout domains 1 and 2, coupled with modeling studies. Within domain 1 a cluster of residues (Glu37, Leu66, Ser68, and Gln75), that are predicted to lie on the CC'FG face of the Ig fold, play a dominant role in LFA-1 binding.


Subject(s)
Antigens, CD , Antigens, Differentiation , Cell Adhesion Molecules/metabolism , Lymphocyte Function-Associated Antigen-1/metabolism , Amino Acid Sequence , Animals , Base Sequence , Binding Sites , Cell Line , DNA Primers , Humans , Models, Molecular , Molecular Sequence Data , Mutagenesis, Site-Directed , Sequence Homology, Amino Acid
6.
J Cell Biol ; 126(2): 529-37, 1994 Jul.
Article in English | MEDLINE | ID: mdl-7518468

ABSTRACT

To analyze the binding requirements of LFA-1 for its two most homologous ligands, ICAM-1 and ICAM-3, we compared the effects of various LFA-1 activation regimes and a panel of anti-LFA-1 mAbs in T cell binding assays to ICAM-1 or ICAM-3 coated on plastic. These studies demonstrated that T cell binding to ICAM-3 was inducible both from the exterior of the cell by Mn2+ and from the interior by an agonist of the "inside-out" signaling pathway. T cells bound both ICAM ligands with comparable avidity. A screen of 29 anti-LFA-1 mAbs led to the identification of two mAbs specific for the alpha subunit of LFA-1 which selectively blocked adhesion of T cells to ICAM-3 but not ICAM-1. These two mAbs, YTH81.5 and 122.2A5, exhibited identical blocking properties in a more defined adhesion assay using LFA-1 transfected COS cells binding to immobilized ligand. Blocking was not due to a steric interference between anti-LFA-1 mAbs and N-linked carbohydrate residues present on ICAM-3 but not ICAM-1. The epitopes of mAbs YTH81.5 and 122.2A5 were shown to map to the I domain of the LFA-1 alpha subunit. A third I domain mAb, MEM-83, has been previously reported to uniquely activate LFA-1 to bind ICAM-1 (Landis, R. C., R. I. Bennett, and N. Hogg. 1993. J. Cell Biol. 120:1519-1527). We now show that mAb MEM-83 is not able to stimulate binding of T cells to ICAM-3 over a wide concentration range. Failure to induce ICAM-3 binding by mAb MEM-83 was not due to a blockade of the ICAM-3 binding site on LFA-1. This study has demonstrated that two sets of functionally distinct mAbs recognizing epitopes in the I domain of LFA-1 are able to exert differential effects on the binding of LFA-1 to its ligands ICAM-1, and ICAM-3. These results suggest for the first time that LFA-1 is capable of binding these two highly homologous ligands in a selective manner and that the I domain plays a role in this process.


Subject(s)
Antigens, CD/metabolism , Antigens, Differentiation , Cell Adhesion Molecules/metabolism , Cell Adhesion/physiology , Lymphocyte Function-Associated Antigen-1/metabolism , T-Lymphocytes/metabolism , Antibodies, Monoclonal , Cells, Cultured , Epitopes/metabolism , Glycosylation , Humans , Intercellular Adhesion Molecule-1 , Ligands , Lymphocyte Function-Associated Antigen-1/chemistry , Lymphocyte Function-Associated Antigen-1/genetics , Lymphocyte Function-Associated Antigen-1/immunology , Manganese/metabolism , Phorbol Esters/metabolism , Protein Structure, Tertiary , Recombinant Fusion Proteins/metabolism , Sequence Deletion
7.
J Biol Chem ; 268(13): 9661-6, 1993 May 05.
Article in English | MEDLINE | ID: mdl-8486654

ABSTRACT

Macrosialin is a heavily glycosylated transmembrane protein of 87-115 kDa, highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. We have isolated cDNA clones encoding macrosialin from a thioglycollate-elicited peritoneal macrophage cDNA library by transient expression in COS cells and panning with the anti-macrosialin monoclonal antibody FA/11. A single 1.3-kilobase macrosialin transcript was detected in both untreated and phorbol 12-myristate 13-acetate-stimulated RAW cells. The cDNA sequence predicts a type I integral membrane protein of 326 residues with a heavily glycosylated extracellular domain of 306 residues containing nine potential N-linked glycosylation sites and numerous potential O-linked glycosylation sites. The extracellular domain consists of two distinct regions, separated by an extended 12 residue proline-rich hinge; a membrane-distal mucin-like domain of 89 residues containing short peptide repeats and consisting of 44% serine and threonine residues; and a membrane proximal domain of 170 residues, which has significant sequence homology to a family of lysosomal associated glycoproteins known as the lamp-1 group. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68 (72% identity, 80% similarity). Both proteins are preferentially expressed by macrophages and share the same bipartite structure having a mucin-like domain and a domain common to the lamp family. Macrosialin and CD68 are the first examples of a lamp family protein with a restricted cell-type-specific expression. They may have evolved from the lamps to carry out specialized functions in dedicated phagocytic cells.


Subject(s)
DNA/genetics , Macrophages/metabolism , Membrane Glycoproteins/genetics , Amino Acid Sequence , Animals , Antigens, CD/genetics , Antigens, Differentiation, Myelomonocytic/genetics , Base Sequence , Blotting, Northern , Cell Line , Cells, Cultured , Cloning, Molecular , Gene Library , Humans , Lysosomal Membrane Proteins , Macrophages/drug effects , Membrane Glycoproteins/biosynthesis , Membrane Glycoproteins/chemistry , Mice , Mice, Inbred BALB C , Molecular Sequence Data , Oligodeoxyribonucleotides , Plasmids , Polymerase Chain Reaction/methods , Protein Conformation , RNA, Messenger/analysis , RNA, Messenger/genetics , RNA, Messenger/metabolism , Rats , Sequence Homology, Amino Acid , Tetradecanoylphorbol Acetate/pharmacology , Transfection
8.
Virology ; 193(2): 672-9, 1993 Apr.
Article in English | MEDLINE | ID: mdl-8460480

ABSTRACT

The location of nucleotide sequences important in determining the extent of cowpea mosaic virus M-RNA accumulation in cowpea protoplasts has been analyzed by deletion mutagenesis of full-length cDNA clones from which infectious transcripts can be produced in vitro. The results suggest that cis-acting sequences which direct replication of M-RNA by B-RNA-encoded products are located within the 5'-terminal 524 nucleotides and the 3'-terminal 151 nucleotides. RNA secondary structure predictions for the 3'-terminal 151 nucleotides of both genomic RNAs (Eggen et al. (1989) Virology 173, 456-464) indicate that the terminal nucleotides form a stable secondary structure composed of a Y-shaped stem-loop and a simple A-U-rich stem-loop. The latter structure has been implicated in B-RNA replication. We have examined the role of the Y-shaped structure in M-RNA accumulation by site-directed mutagenesis of putative base-pairing combinations in the two minor stems. The results suggest that efficient replication is dependent on the formation of both of these minor stem structures.


Subject(s)
Mosaic Viruses/genetics , RNA, Viral/metabolism , Base Sequence , Blotting, Northern , Capsid/genetics , Fabaceae , Molecular Sequence Data , Mosaic Viruses/metabolism , Mutagenesis, Site-Directed , Nucleic Acid Conformation , Oligodeoxyribonucleotides , Plants, Medicinal , Point Mutation , RNA, Viral/chemistry , RNA, Viral/genetics , Restriction Mapping , Sequence Deletion
9.
Blood ; 81(6): 1607-13, 1993 Mar 15.
Article in English | MEDLINE | ID: mdl-7680921

ABSTRACT

CD68 is a 110-Kd transmembrane glycoprotein of unknown function highly expressed by human monocytes and tissue macrophages. We have isolated cDNA clones encoding CD68 from a U937 cDNA library by transient expression in COS cells and panning with the anti-CD68 monoclonal antibodies (MoAbs) Y2/131, Y1/82A, EBM11, and Ki-M6. CD68 transcripts are constitutively present in the promonocyte cell line U937 and are upregulated by phorbol myristic acid (PMA). By contrast, CD68 transcripts are absent or present at very low levels in many hematopoietic lines including KG1, CEM, and K562, but can be induced by exposure to PMA. The cDNA sequence predicts a type I integral membrane protein of 354 residues with a heavily glycosylated extracellular domain of 298 residues containing nine potential N-linked glycosylation sites and numerous potential O-linked glycosylation sites. The extracellular domain consists of two distinct regions separated by an extended proline hinge: a membrane-distal mucin-like domain containing short peptide repeats and consisting of 54% serine and threonine residues; and a membrane proximal domain that has significant sequence homology to a family of lysosomal/plasma membrane shuttling proteins known as the lamp 1 group. CD68 is a member of a growing family of hematopoietic mucin-like molecules, including leukosialin/CD43, the stem cell antigen CD34, and the lymph node high endothelial ligand for L-selectin GlyCAM-1.


Subject(s)
Antigens, CD/genetics , Antigens, Differentiation, Myelomonocytic/genetics , Cloning, Molecular , Amino Acid Sequence , Antigens, CD/chemistry , Antigens, Differentiation, Myelomonocytic/chemistry , Base Sequence , Glycosylation , Humans , Lysosomal Membrane Proteins , Membrane Glycoproteins/chemistry , Membrane Glycoproteins/genetics , Molecular Sequence Data , P-Selectin , Platelet Membrane Glycoproteins/metabolism , Sequence Alignment
10.
Nature ; 360(6403): 481-4, 1992 Dec 03.
Article in English | MEDLINE | ID: mdl-1448173

ABSTRACT

The co-ordinated function of effector and accessory cells in the immune system is assisted by adhesion molecules on the cell surface that stabilize interactions between different cell types. Leukocyte function-associated antigen 1 (LFA-1) is expressed on the surface of all white blood cells and is a receptor for intercellular adhesion molecules (ICAM) 1 and 2 (ref. 3) which are members of the immunoglobulin superfamily. The interaction of LFA-1 with ICAMs 1 and 2 provides essential accessory adhesion signals in many immune interactions, including those between T and B lymphocytes and cytotoxic T cells and their targets. In addition, both ICAMs are expressed at low levels on resting vascular endothelium; ICAM-1 is strongly upregulated by cytokine stimulation and plays a key role in the arrest of leukocytes in blood vessels at sites of inflammation and injury. Recent work has indicated that resting leukocytes express a third ligand, ICAM-3, for LFA-1 (refs 11, 12). ICAM-3 is potentially the most important ligand for LFA-1 in the initiation of the immune response because the expression of ICAM-1 on resting leukocytes is low. We report the expression cloning of a complementary DNA, pICAM-3, encoding a protein constitutively expressed on all leukocytes, which binds LFA-1. ICAM-3 is closely related to ICAM-1, consists of five immunoglobulin domains, and binds LFA-1 through its two N-terminal domains.


Subject(s)
Antigens, CD , Antigens, Differentiation , Cell Adhesion Molecules/genetics , Leukocytes/physiology , Lymphocyte Function-Associated Antigen-1/metabolism , Amino Acid Sequence , Animals , B-Lymphocytes , Base Sequence , Cell Adhesion Molecules/isolation & purification , Cell Adhesion Molecules/metabolism , Cell Line , Cells, Cultured , Cloning, Molecular , Endothelium, Vascular/physiology , Exons , Gene Library , Humans , Immunoglobulin G/genetics , Ligands , Lymphocyte Function-Associated Antigen-1/isolation & purification , Molecular Sequence Data , Oligodeoxyribonucleotides , Polymerase Chain Reaction , Recombinant Proteins/isolation & purification , Recombinant Proteins/metabolism , Sequence Homology, Amino Acid , Transfection , Tumor Cells, Cultured
11.
Virology ; 172(1): 311-20, 1989 Sep.
Article in English | MEDLINE | ID: mdl-2773321

ABSTRACT

A full-length cDNA copy of CPMV M RNA has been cloned downstream of a phage lambda promoter in the plasmid pPMI. Transcripts obtained from this clone can be translated in vitro and replicated in cowpea mesophyll protoplasts in the presence of viral B RNA. We have constructed a series of site-directed mutants of this clone to investigate the mechanism of translation of CPMV M RNA. The results obtained confirm that the AUG at position 161 is used to direct the synthesis of a 105K protein in vitro and the detection of a 58K protein in infected cowpea protoplasts suggests that it is also used in vivo. The synthesis of the 95K protein can be initiated from either of the AUGs at positions 512 and 524, though synthesis of this protein does not appear to be essential for CPMV replication in protoplasts.


Subject(s)
Mosaic Viruses/genetics , Peptide Chain Initiation, Translational , RNA, Messenger/genetics , RNA, Viral/genetics , Blotting, Northern , Blotting, Western , Cloning, Molecular , Codon , DNA/genetics , DNA Mutational Analysis , Genetic Vectors , Molecular Weight , Transcription, Genetic , Viral Proteins/genetics
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