Reduced dry season transpiration is coupled with shallow soil water use in tropical montane forest trees.

Tropical montane cloud forests (TMCF) are ecosystems particularly sensitive to climate change; however, the effects of warmer and drier conditions on TMCF ecohydrology remain poorly understood. To investigate functional responses of TMCF trees to reduced water availability, we conducted a study during the 2014 dry season in the lower altitudinal limit of TMCF in central Veracruz, Mexico. Temporal variations of transpiration, depth of water uptake and tree water sources were examined for three dominant, brevi-deciduous species using micrometeorological, sap flow and soil moisture measurements, in combination with oxygen and hydrogen stable isotope composition of rainfall, tree xylem, soil and stream water. Over the course of the dry season, reductions in crown conductance and transpiration were observed in canopy species (43 and 34%, respectively) and mid-story trees (23 and 8%), as atmospheric demand increased and soil moisture decreased. Canopy species consistently showed more depleted isotope values compared to mid-story trees. However, MixSIAR Bayesian model results showed that the evaporated (enriched) soil water pool was the main source for trees despite reduced soil moisture. Additionally, while increases in tree water uptake from deeper to shallower soil water sources occurred, concomitant decreases in transpiration were observed as the dry season progressed. A larger reduction in deep soil water use was observed for canopy species (from 79 ± 19 to 24 ± 20%) compared to mid-story trees (from 12 ± 17 to 10 ± 12%). The increase in shallower soil water sources may reflect a trade-off between water and nutrient requirements in this forest.

Why size matters: the interactive influences of tree diameter distribution and sap flow parameters on upscaled transpiration.

In stands with a broad range of diameters, a small number of very large trees can disproportionately influence stand basal area and transpiration (Et). Sap flow-based Et estimates may be particularly sensitive to large trees due to nonlinear relationships between tree-level water use (Q) and tree diameter at breast height (DBH). Because Q is typically predicted on the basis of DBH and sap flow rates measured in a subset of trees and then summed to obtain Et, we assessed the relative importance of DBH and sap flow variables (sap velocity, Vs, and sapwood depth, Rs) in determining the magnitude of Et and its dependence on large trees in a tropical montane forest ecosystem. Specifically, we developed a data-driven simulation framework to vary the relationship between DBH and Vs and stand DBH distribution and then calculate Q, Et and the proportion of Et contributed by the largest tree in each stand. Our results demonstrate that variation in how Rs is determined in the largest trees can alter estimates up to 26% of Et while variation in how Vs is determined can vary results by up to 132%. Taken together, these results highlight a great need to expand our understanding of water transport in large trees as this hinders our ability to predict water fluxes accurately from stand to catchment scales.

Foggy days and dry nights determine crown-level water balance in a seasonal tropical Montane cloud forest.

The ecophysiology of tropical montane cloud forest (TMCF) trees is influenced by crown-level microclimate factors including regular mist/fog water inputs, and large variations in evaporative demand, which in turn can significantly impact water balance. We investigated the effect of such microclimatic factors on canopy ecophysiology and branch-level water balance in the dry season of a seasonal TMCF in Veracruz, Mexico, by quantifying both water inputs (via foliar uptake, FU) and outputs (day- and night-time transpiration, NT). Measurements of sap flow, stomatal conductance, leaf water potential and pressure-volume relations were obtained in Quercus lanceifolia, a canopy-dominant tree species. Our results indicate that FU occurred 34% of the time and led to the recovery of 9% (24 ± 9.1 L) of all the dry-season water transpired from individual branches. Capacity for FU was independently verified for seven additional common tree species. NT accounted for approximately 17% (46 L) of dry-season water loss. There was a strong correlation between FU and the duration of leaf wetness events (fog and/or rain), as well as between NT and the night-time vapour pressure deficit. Our results show the clear importance of fog and NT for the canopy water relations of Q. lanceifolia.