ABSTRACT
Morphology in combination with flow cytometry is an inexpensive and fast tool to characterize important reasons for early gestational loss. Early partial hydatidiform moles are especially difficult to identify as not all have developed the typical histological features of central cistern formation and trophoblastic hyperplasia of the chorionic villi. Angiomatoid formation of the blood vessels, irregular chorionic villi, diffuse villous stromal fibrosis, trophoblastic pseudoinclusions, and pleomorphic trophoblast at the implantation site have therefore been proposed as additional morphological criteria. In our study, we investigated the correlation between morphological features and results of ploidy analysis to assess the additional criteria for practical use. Morphological features of the placentas of up to 13 weeks of gestation were evaluated on hematoxylin and eosin sections. Ploidy analysis was performed by flow cytometry on formalin-fixed, paraffin-embedded tissue. Statistical analysis was performed by binary logistic regression. One hundred fifty samples were included in the study. Of the samples, 23 were triploid, 19 were tetraploid, and the remaining ones were diploid. Statistical analysis showed a poor predictive power based on all morphological criteria alone. The most important result of this study was the identification of five triploid placentas that showed diffuse fibrosis of the chorionic villi, angiomatoid changes of the blood vessels, trophoblastic pseudoinclusions, and focal pleomorphic changes at the implantation site, but none of the classical morphological features of triploid pregnancies were identified. Our study demonstrates that the use of additional morphological criteria increases the number of missed abortions with identified causes for pregnancy loss.
Subject(s)
Abortion, Spontaneous/genetics , Abortion, Spontaneous/pathology , Placenta/pathology , Ploidies , Pregnancy Trimester, First , Abortion, Missed/epidemiology , Chromosome Aberrations , Female , Flow Cytometry , Humans , Hydatidiform Mole/genetics , Hydatidiform Mole/pathology , Hyperplasia , Incidence , Logistic Models , PregnancyABSTRACT
PURPOSE: To compare the obstetric and neonatal outcome in pregnancies complicated by gestational diabetes mellitus after amniocentesis for amniotic fluid insulin measurement or maternal blood glucose monitoring or both as selection criterion for therapy. MATERIAL AND METHODS: In a retrospective study, 408 diabetic pregnancies were analyzed; 307 were treated with diet alone (group 1) and 101 with diet and insulin (group 2). Pearson's chi2 or Fisher's exact test was used to assess obstetric and anthropometric data within these groups, and p values <0.05 were considered statistically significant. RESULTS: Group 1 - According to the method used, no significant differences were found for gestational age at birth (p = 0.396), mode of delivery (p = 0.79) and neonatal outcome parameters determined as mean birth weight (p = 0.348), birth weight above the 75th percentile (p = 0.473), mean ponderal index (PI; p = 0.434), pH of umbilical artery (p = 0.065) and of umbilical-vein blood (p = 0.052), mean Apgar scores at 1 (p = 0.56) and 5 min (p = 0.072), insulin (p = 0.25) and glucose (p = 0.535) in cord blood. Significant differences were found for birth weight above the 90th percentile (p = 0.005) and Apgar score <7 at 1 min (p = 0.019). Group 2 - Again, no significant differences were observed in terms of gestational age at birth (p = 0.219), mode of delivery (p = 0.386), mean birth weight (p = 0.59), birth weight above the 75th (p = 0.701) and 90th percentiles (p = 0.487), mean PI (p = 0.156), pH of umbilical-artery (p = 0.197) and umbilical vein blood (p = 0.056), Apgar scores at 1 (p = 0.58) and 5 min (p = 0.52), insulin (p = 0.67) and glucose (p = 0.11) in cord blood. CONCLUSION: In retrospective analysis there was no significant difference in outcome parameters in pregnancies complicated by gestational diabetes dependent on the method used as selection criterion for therapy.
