ABSTRACT
The monoclonal IgG2a-antibody RoMo-1 binds to peripheral human blood monocytes but does not react with other blood cells, tissue macrophages, HL-60, K 562 or U 937. It seems possible, that the antibody defines an antigen expressed on the premonocytic and monocyte stages of cells from the mononuclear phagocytic system only. The antibody is cytotoxic.
Subject(s)
Antibodies, Monoclonal/immunology , Monocytes/immunology , Antibody Specificity , Cell Adhesion , Cell Line , Granulocytes/immunology , Humans , Immunoenzyme Techniques , Macrophages/immunology , Pulmonary Alveoli/immunology , Rosette Formation , T-Lymphocytes/immunologyABSTRACT
Purified human lymphocytes from peripheral blood were stimulated by Con A and the concentrated supernatants were used to induce antibodies in rabbits. These antilymphokine immune sera (ALKS) were able to inhibit the electrophoretic mobility of indicator cells, which was performed using supernatants of lymphocytes stimulated by Con A or PPD and tanned sheep red blood cells as indicator particles. If the method is standardized it is possible to compare several ALKS from different origin even in different models.
Subject(s)
Immune Sera , Lymphokines/immunology , Concanavalin A/pharmacology , Electrophoresis , Humans , Lymphocytes/drug effects , Lymphocytes/immunologyABSTRACT
Human purified lymphocytes from peripheral blood were stimulated by Con A and the concentrated supernatants were used to induce antibodies in rabbits. These anti-lymphokine immune sera were able to inhibit the electrophoretic-mobility-test (EMT) which was performed using supernatants of lymphocytes stimulated by Con A and tanned sheep red blood cells as indicator particles. Since the inhibition is strongly dose dependent the test system appears to demonstrate and compare anti-lymphokine activities in different sera. When anti-human lymphocyte globulin (Dessau, DDR) was tested in the same test system these sera had the capacity to inhibit the EMT completely.
