ABSTRACT
OBJECTIVE: To assess high-risk human papillomavirus (hrHPV) infection among women undergoing cervical cancer screening in Putian for establishing an optimal cervical cancer screening mode and preventive vaccination strategy for HPV. STUDY DESIGN: Cross-sectional study. Place and Duration of the Study: The Affiliated Hospital of Putian University for cervical cancer screening period, from August 2020 to December 2022. METHODOLOGY: Cervical cell specimens were obtained using 'two cancer screening platforms'. qRT-PCR and flow-FISH were used for hrHPV typing. The pathological diagnostic test was performed for the hrHPV-positive samples. The results concerning the relationships between hrHPV infection at different age groups and pathological diagnosis were analysed retrospectively. RESULTS: A total of 98085 hrHPV preliminary screening results in the Putian region and 9036 hrHPV-positive samples were included. The infection rate of hrHPV for the three infection modes increased with age. The 41-50 age group is the highest incidence which the phase from cervical intraepithelial neoplasia to cervical cancer. The top three hrHPV subtypes were HPV52, HPV58, and HPV16. The positive rate of HPV16 was positively correlated with the progression of cervical intraepithelial neoplasia. CONCLUSION: Effective screening, vaccination, and education must be provided because HPV infections are district-specific and age-specific. HPV16 is correlated with cervical cancer progression. Pathological diagnosis and prevention of cervical cancer infected with HPV16 must be conducted. KEY WORDS: hrHPV, Cervical cancer, Pathological diagnosis.
Subject(s)
Papillomavirus Infections , Uterine Cervical Dysplasia , Uterine Cervical Neoplasms , Female , Humans , Uterine Cervical Neoplasms/diagnosis , Uterine Cervical Neoplasms/epidemiology , Uterine Cervical Neoplasms/prevention & control , Cross-Sectional Studies , Human Papillomavirus Viruses , Retrospective Studies , Early Detection of Cancer/methods , Uterine Cervical Dysplasia/diagnosis , China/epidemiology , Papillomavirus Infections/diagnosis , Papillomavirus Infections/epidemiology , Papillomavirus Infections/prevention & control , Human papillomavirus 16 , Papillomaviridae/genetics , GenotypeABSTRACT
BACKGROUND: Balanced translocation of chromosomes has a negative impact on male fertility, which can easily cause clinical manifestations such as oligospermia and asthenospermia. It is necessary to conduct cytogenetic examination on men of childbearing age to guide them in their fertility. METHODS: We report a case of balanced translocation: 46, XY, t(9;22) (q22;q13) accompanied with oligospermia and asthenospermia. The lymphocytes in peripheral blood were cultured to examine the patient's karyotype. RESULTS: The karyotypes of the patient and the patient's wife were detected and identified as 46, XY, t(9;22) (9pterâ9q22::22q13â22qter;22pterâ22q13::9q22â9qter) and 46, XX, respectively. The origin of the chromosome translocation was unknown because the patient's parents did not undergo cytogenetic tests. CONCLUSIONS: For patients with oligospermia and asthenospermia, cytogenetic examination should be carried out to obtain a healthy fetus. Prenatal diagnosis should be strictly performed to prevent the birth of children with chromosomal diseases if one partner of the couple is a carrier with abnormal chromosome structure.
