Lipidomic Analysis of Cervicovaginal Fluid for Elucidating Prognostic Biomarkers and Relevant Phospholipid and Sphingolipid Pathways in Preterm Birth.

Cervicovaginal fluid (CVF) is an excellent specimen for monitoring preterm birth (PTB) as it characterizes cervical metabolites, the vaginal environment, and specific host immune responses. However, extensive lipid analysis of CVF to explain PTB has not been studied. In this study, we performed a systematic analysis combining high-throughput lipid analysis and omics to discover the unique metabolic properties of the cervix. Liquid chromatography-high resolution mass spectrometry successfully detected a total of 190 lipids in the CVF of 30 PTB and 30 term birth (TB) pregnant women. The whole lipidomics dataset analyzed by combining multivariate and univariate statistical analysis revealed 35 lipid biomarkers, including phospholipids and sphingolipids. Remarkably, sphingomyelin, which plays a physiologically essential role in sphingolipids, was significantly downregulated in PTB. Metabolic pathway study provides a close relationship between vaginal microbial organization and cell membrane formation, further supporting the robustness of our findings. Sphingolipids and phospholipids, which were determined to be important lipids for predicting PTB in our study, showed a high value of receiver operating characteristic (ROC) curve >0.7, indicating that a lipid diagnostic test and understanding the mechanism of lipids is highly related to the vaginal microbiome. Therefore, our result has high potential as a predictor of PTB.

Hepatocellular Metabolic Abnormalities Induced by Long-Term Exposure to Novel Brominated Flame Retardant, Hexabromobenzene.

Novel brominated flame retardants (NBFRs) are widely used to avoid environmental accumulation concerns and because of the regulations imposed on classical BFRs. However, recent studies have not revealed the negative effects of NBFR accumulation and exposure on humans. We conducted a metabolomics study on hexabromobenzene (HBB), one of the NBFRs, to investigate its effect on hepatocytes. Gas chromatography-mass spectrometry-based metabolite profiling was performed to observe metabolic perturbations by treating human livertissue-derived HepG2 cell lines with HBB for maximum 21 days. Metabolic pathway enrichment using 17 metabolite biomarkers determined via univariate and multivariate statistical analysis verified that long-term accumulation of HBB resulted in distinct diminution of eight amino acids and five other metabolites. Molecular docking of the biomarker-related enzymes revealed the potential molecular mechanism of hepatocellular response to HBB exposure, which disrupts the energy metabolism of hepatic cells. Collectively, this study may provide insights into the hidden toxicity of bioaccumulating HBB and unveil the risks associated with non-regulated NBFRs.

Development of a New Biomarker Model for Predicting Preterm Birth in Cervicovaginal Fluid.

Preterm birth (PTB) is a social problem that adversely affects not only the survival rate of the fetus, but also the premature babies and families, so there is an urgent need to find accurate biomarkers. We noted that among causes, eubiosis of the vaginal microbial community to dysbiosis leads to changes in metabolite composition. In this study, short chain fatty acids (SCFAs) representing dysbiosis were derivatized using (N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide, MTBSTFA) and targeted analysis was conducted in extracted organic phases of cervicovaginal fluid (CVF). In residual aqueous CVF, polar metabolites produced biochemistry process were derivatized using methoxyamine and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA), and non-targeted analysis were conducted. Nine SCFAs were quantified, and 58 polar metabolites were detected in 90 clinical samples using gas chromatography/mass spectrometry (GC/MS). The criteria of statistical analysis and detection rate of clinical sample for development of PTB biomarkers were presented, and 19 biomarkers were selected based on it, consisting of 1 SCFA, 2 organic acids, 4 amine compounds, and 12 amino acids. In addition, the model was evaluated as a suitable indicator for predicting PTB without distinction between sample collection time. We hope that the developed biomarkers based on microbiota-derived metabolites could provide useful diagnostic biomarkers for actual patients and pre-pregnancy.

Mechanical and Electrical Characteristics of Lightweight Aggregate Concrete Reinforced with Steel Fibers.

There is increased interest in applying electromagnetic (EM) shielding to prevent EM interference, which destroys electronic circuits. The EM shielding's performance is closely related to the electrical conductivity and can be improved by incorporating conductive materials. The weight of a structure can be reduced by incorporating lightweight aggregates and replacing the steel rebars with CFRP rebars. In this study, the effects of lightweight coarse aggregate and CFRP rebars on the mechanical and electrical characteristics of concrete were investigated, considering the steel fibers' incorporation. The lightweight coarse aggregates decreased the density and strength of concrete and increased the electrical conductivity of the concrete, owing to its metallic contents. The steel fibers further increased the electrical conductivity of the lightweight aggregate concrete. These components improved the EM shielding performance, and the steel fibers showed the best performance by increasing shielding effectiveness by at least 23 dB. The CFRP rebars behaved similarly to steel rebars because of their carbon fiber content. When no steel fiber was mixed, the shielding effectiveness increased by approximately 2.8 times with reduced spacing of CFRP rebars. This study demonstrates that lightweight aggregate concrete reinforced with steel fibers exhibits superior mechanical and electrical characteristics for concrete and construction industries.

Evaluation on the Microstructure and Durability of High-Strength Concrete Containing Electric Arc Furnace Oxidizing Slag.

The application of electric arc furnace oxidizing slag (EAS) in high strength concrete (HSC) as the cementitious material is investigated in this study. The microstructure and mechanical properties of HSC with four different replacement ratios of EAS were evaluated and HSC with two replacement ratios of ground granulated blast furnace slag (GBS) was used for performance comparison. The results show that the HSC with EAS replacement ratios smaller than 15% undergo similar hydration processes and result in a similar final product when compared with those of NC-NN. Increases in EAS replacement ratio cause a reduction in Ca(OH)2 content; this, in turn, leads to an increase in porosity and a reduction in compressive strength. In terms of shrinkage behavior under free conditions, mixtures with increasing replacement ratios of cementitious materials saw increasing shrinkage, with the HSC containing EAS being similar to the other specimens. The mixtures containing EAS saw a quite gradual decrease in their freezing and thawing resistance properties as the number of freeze-thaw cycles they underwent increased. However, the efficacy of HSC with less than 15% of EAS is similar to GBS; hence, EAS could replace cement in concrete for certain applications, which would lead to more environmental benefits.

Effect of Strengthening Methods on Two-Way Slab under Low-Velocity Impact Loading.

In this study, the performance of reinforced concrete slabs strengthened using four methods was investigated under impact loads transferred from the top side to bottom side. The top and bottom sides of test slabs were strengthened by no-slump high-strength, high-ductility concrete (NSHSDC), fiber-reinforced-polymer (FRP) sheet, and sprayed FRP, respectively. The test results indicated that the test specimens strengthened with FRP series showed a 4% increase in reaction force and a decrease in deflection by more than 20% compared to the non-strengthened specimens. However, the specimen enhanced by the NSHSDC jacket at both the top and bottom sides exhibited the highest reaction force and energy dissipation as well as the above measurements because it contains two types of fibers in the NSHSDC. In addition, the weight loss rate was improved by approximately 0.12% for the NSHSDC specimen, which was the lowest among the specimens when measuring the weight before and after the impact load. Therefore, a linear relationship between the top and bottom strengthening of the NSHSDC and the impact resistance was confirmed, concluding that the NSHSDC is effective for impact resistance when the top and bottom sides are strengthened. The results of the analysis of the existing research show that the NSHSDC is considered to have high impact resistance, even though it has lower resistance than the steel fiber reinforced concrete and ultra-high-performance-concrete, it can be expected to further studies on strengthening of NSHSDC.

Bond Strength and Flexural Capacity of Normal Concrete Beams Strengthened with No-Slump High-Strength, High-Ductility Concrete.

This study investigates the flexural behavior of normal-strength concrete (NSC) beams that were strengthened with no-slump, high-strength, high-ductility concrete (NSHSDC). A set of slant shear tests was performed to investigate the initial performance of the NSC substrate strengthened with NSHSDC. Slant shear tests considered two types of roughness of interface and five angles of the interface between NSC and NSHSDC. The test results showed that except for specimens with a 75° interface angle, the specimens with high roughness were conformed to the properties (14-21 MPa for 28 days) of the ACI Committee 546 recommendation. For flexural strength tests, NSC beams strengthened with an NSHSDC jacket on the top and bottom sides, three sides, and four sides resulted in strength increments of about 8%, 29%, and 40%, respectively, compared to the beams without NSHSDC jacket. Therefore, the use of NSHSDC is an effective method to improve the performance of NSC beams and is recommended for strengthening reinforced concrete members.

Effects of Steelmaking Slag and Moisture on Electrical Properties of Concrete.

Strain sensors can indicate the conditions of concrete structures, but these sensors are only capable of measuring local behaviors of materials. To solve this problem, researchers have introduced conductive materials that can monitor the overall behavior of concrete structures. Steelmaking slag, which contains large amounts of iron oxide (Fe2O3), is conductive, and researchers have considered the addition of this material to improve concrete monitoring. In this study, mechanical and electrical properties of concrete containing steelmaking slag as a binder were evaluated. As the incorporation of steelmaking slag increased, the setting times were delayed, but the compressive strengths were similar within the replacement ratio of 15%. It was found that the addition of steelmaking slag with Fe2O3, the main ingredient of magnetite (Fe3O4), improved the electrical resistivity, piezoresistivity, and sensitivity of the concrete. Drying of the concretes resulted in an increase in electrical resistance and fractional change in resistivity (FCR). Expansion of steelmaking slag, due to contacting of free CaO and moisture under repeated loads, resulted in cracks in the concrete and affected the gauge factor (GF). This study demonstrates the possibility that the addition of steelmaking slag as a binder may provide an economical and environmentally-friendly solution to concrete strain monitoring.