Global drivers of the conservation-invasion paradox.

The conservation-invasion paradox (CIP) refers to a long-term phenomenon wherein species threatened in their native range can sustain viable populations when introduced to other regions. Understanding the drivers of CIP is helpful for conserving threatened species and managing invasive species, which is unfortunately still lacking. We compiled a global data set of 1071 introduction events, including 960 CIP events (successful establishment of threatened species outside its native range) and 111 non-CIP events (unsuccessful establishment of threatened species outside its native range after introduction), involving 174 terrestrial vertebrates. We then tested the relative importance of various predictors at the location, event, and species levels with generalized linear mixed models and model averaging. Successful CIP events occurred across taxonomic groups and biogeographic realms, especially for the mammal group in the Palearctic and Australia. Locations of successful CIP events had fewer native threat factors, especially less climate warming in invaded regions. The probability of a successful CIP event was highest when species introduction efforts were great and there were more local congeners and fewer natural enemies. These results can inform threatened species ex situ conservation and non-native invasive species mitigation.

Causantes mundiales de la paradoja conservación­invasión Resumen La paradoja de conservación­invasión (PCI) se refiere al evento a largo plazo en el que las especies amenazadas en su distribución nativa puedan mantener poblaciones viables cuando se les introduce a otras regiones. Es de mucha ayuda para la conservación de especies amenazadas y el manejo de especies invasoras entender las causantes de la PCI, entendimiento que todavía es escaso. Compilamos un conjunto mundial de datos de 174 vertebrados terrestres en 1071 eventos de introducción, incluyendo 960 eventos de PCI (el establecimiento exitoso de especies amenazadas fuera de su distribución nativa) y 111 eventos no PCI (el fracaso en el establecimiento de especies amenazadas fuera de su distribución nativa después de la introducción). Después analizamos con modelos lineales mixtos generalizados y promedio de modelos la importancia relativa de varios pronosticadores en la localidad, en el evento y a nivel de especie. Los eventos exitosos de PCI ocurrieron en todos los grupos taxonómicos y en todos los reinos biogeográficos, especialmente para los mamíferos del Paleártico y Australia. Las localidades de los eventos exitosos de PCI tuvieron menos factores nativos de amenaza, especialmente un menor calentamiento climático en las regiones invadidas. La probabilidad de que un evento de PCI sea exitoso fue mayor cuando los esfuerzos de introducción fueron mayores y hubo más congéneres locales y menos enemigos naturales. Estos resultados pueden orientar la conservación ex situ de especies y la mitigación de especies invasoras no nativas.

Synergistic effect of two bacterial strains promoting anaerobic digestion of rice straw to produce methane.

A large amount of agricultural waste causes global environmental pollution. Biogas production by microbial pretreatment is an important way to utilize agricultural waste resources. In this study, Sporocytophaga CG-1 (A, cellulolytic strain) was co-cultured with Bacillus clausii HP-1 (B, non-cellulolytic strain) to analyze the effect of pretreatment of rice straw on methanogenic capacity of anaerobic digestion (AD). The results showed that weight loss rate of filter paper of co-culture combination is 53.38%, which is 29.37% higher than that of A. The synergistic effect of B on A can promote its degradation of cellulose. The cumulative methane production rate of the co-culture combination was the highest (93.04 mL/g VS substrate), which was significantly higher than that of A, B and the control group (82.38, 67.28 and 67.70 mL/g VS substrate). Auxiliary bacteria can improve cellulose degradation rate by promoting secondary product metabolism. These results provide data support for the application of co-culture strategies in the field of anaerobic digestion practices.

Synergistic analysis of lignin degrading bacterial consortium and its application in rice straw fiber film.

Fiber film have received widespread attention due to its green friendliness. We can use microorganisms to degrade lignin in straw to obtain cellulose and make fiber films. Herein, a group of high-temperature (50 °C) lignin degrading bacterial consortium (LDH) was enriched and culture conditions for lignin degradation were optimized. Combined with high-throughput sequencing technology, the synergistic effect of LDH-composited bacteria was analyzed. Then LDH was used to treat rice straw for the bio-pulping experiment. The results showed that the lignin of rice straw was degraded 32.4 % by LDH at 50 °C for 10 d, and after the optimization of culture conditions, lignin degradation rate increased by 9.05 % (P < 0.001). The bacteria that compose in LDH can synergistically degrade lignin. Paenibacillus can encode all lignin-degrading enzymes present in the LDH. Preliminary tests of LDH in the pulping industry have been completed. This study is the first to use high temperature lignin degrading bacteria to fabricate fiber film.

Social media unveils the hidden but high magnitude of human-mediated biological invasions in China.

Humans are responsible for the release of many non-native animals into the wild. However, these releases occur randomly and are difficult to monitor. Here, using two of the worst invasive herpetofauna as model taxa, we applied an iEcology approach and found a high magnitude of human-mediated releases in China, suggesting this approach can be used to monitor introductions and advise management bodies in a timely manner.

Preferred prey reduce species realized niche shift and improve range expansion prediction.

Many studies have detected realized climatic niche shifts during range changes; this is challenging the fundamental theory of the niche conservatism hypothesis (NCH) and the usefulness of the ecological niche model (ENM) for predicting the distributions of species in space and time by tracking environmental change. Biotic factors such as predatory interactions are important components of species realized niches but are generally difficult to quantify during NCH testing and ENM building. Identifying species' preferred prey may provide a unique opportunity to include trophic interactions in assessing the NCH and determine whether more precise ENM predictions are generated. In this study, we focused on a range-expanding predatory bird, the Asian openbill (Anastomus oscitans). The main prey of the Asian openbill include 136 snail species. We observed a realized climatic niche shift during the northward expansion of the Asian openbill by considering only climates; however, niche conservatism was detected after incorporating their preferred prey. ENMs including preferred snails also predicted the distributions of the Asian openbill better than climate-only models and models including nonpreferred snails or only habitat variables. The results of our study suggested the importance of incorporating preferred prey in evaluating the NCH and developing a framework for predicting the range shifts of both native and alien species in response to global climate change.

Time-delay signature suppression in delayed-feedback semiconductor lasers as a paradigm for feedback control in complex physiological networks.

Physiological networks, as observed in the human organism, involve multi-component systems with feedback loops that contribute to self-regulation. Physiological phenomena accompanied by time-delay effects may lead to oscillatory and even chaotic dynamics in their behaviors. Analogous dynamics are found in semiconductor lasers subjected to delayed optical feedback, where the dynamics typically include a time-delay signature. In many applications of semiconductor lasers, the suppression of the time-delay signature is essential, and hence several approaches have been adopted for that purpose. In this paper, experimental results are presented wherein photonic filters utilized in order to suppress time-delay signatures in semiconductor lasers subjected to delayed optical feedback effects. Two types of semiconductor lasers are used: discrete-mode semiconductor lasers and vertical-cavity surface-emitting lasers (VCSELs). It is shown that with the use of photonic filters, a complete suppression of the time-delay signature may be affected in discrete-mode semiconductor lasers, but a remnant of the signature persists in VCSELs. These results contribute to the broader understanding of time-delay effects in complex systems. The exploration of photonic filters as a means to suppress time-delay signatures opens avenues for potential applications in diverse fields, extending the interdisciplinary nature of this study.

Multi-constraint Gerchberg-Saxton iteration algorithms for linearizing IM/DD transmission systems.

Chromatic dispersion-enhanced signal-signal beating interference (SSBI) considerably affects the performance of intensity-modulation and direct-detection (IM/DD) fiber transmission systems. For recovering optical fields from received double sideband signals after propagating through IM/DD transmission systems, Gerchberg-Saxton (G-S) iterative algorithms are promising, which, however, suffers slow convergence speeds and local optimization problems. In this paper, we propose a multi-constraint iterative algorithm (MCIA) to extend the Gerchberg-Saxton-based linearized detection. The proposed technique can accelerate the convergence speed and realize nonlinear-equalization-free detection. Based on the data-aided iterative algorithm (DIA) and the decision-directed data-aided iterative algorithm (DD-DIA), the proposed technique reuses redundant bits from channel coding to not only correct decision errors but also enforce the constraints on the task function to further accelerate the whole optical field retrieval processing. Simulation results show that, compared with the DD-DIA, the MCIA reduces the received optical power (ROP) by about 1.5-dB for a 100-Gb/s over 50-km SSMF PAM-4 signal transmission at the symbol error rate (SER) of 2×10-2. For a 100-Gb/s over 400-km SSMF transmission system, just 30 MCIA iterations is needed, which is 30% reduction in iteration count compared with the DD-DIA. For further increased transmission capacities, the MCIA can improve the SER by two orders of magnitude compared with the conventional IA. To validate the effectiveness of the MCIA, we also conduct experiments to transmit 92-Gb/s PAM-4 signals over 50-km IM/DD fibre systems. We find that the MCIA has a 1-dB ROP improvement compared with the DD-DIA. Compared with Volterra nonlinear equalization, the BERs of the MCIA with a simple linear equalizer are reduced by more than one order of magnitude with only 52 MCIA iterations.

Permutation entropy analysis of the output of a laser diode under stimulated Brillouin scattering optical feedback.

The chaotic output emitted by a diode laser with optical feedback has fascinated the community for decades. The external cavity delay time imparts a weak level of periodicity to the laser output (the so-called "time delay signature", TDS) that is a drawback for applications that require random optical signals. A lot of efforts have focused in suppressing the TDS either by post-processing the signal or by using alternative ways to generate random optical signals. Here, we compare the signals generated by two optical feedback setups: in the first one, the stimulated Brillouin backscattered light from a standard optical fibre is re-injected into the laser (stimulated Brillouin scattering optical feedback, SBSOF); in the second one, the light transmitted through the fibre is re-injected into the laser (conventional optical feedback, COF). We analyse the permutation entropy, a well-known measure of complexity that captures order relations between values of a time series. We find that, on average, the signal generated by the SBSOF setup has slightly lower PE than the one generated by the COF setup, except when the sampling time of the intensity signal is an exact multiple of the delay; in that case, due to TDS, the entropy of the COF signal is lower than that of the SBSOF signal. We interpret the lower entropy value of the SBSOF signal as due to oscillations at the Brillouin frequency shift. Taken together, our results show that TDS suppression can have an undesirable side effect: a decrease of the entropy of the signal.

DFT-Spread Spectrally Overlapped Hybrid OFDM-Digital Filter Multiple Access IMDD PONs.

A novel transmission technique-namely, a DFT-spread spectrally overlapped hybrid OFDM-digital filter multiple access (DFMA) PON based on intensity modulation and direct detection (IMDD)-is here proposed by employing the discrete Fourier transform (DFT)-spread technique in each optical network unit (ONU) and the optical line terminal (OLT). Detailed numerical simulations are carried out to identify optimal ONU transceiver parameters and explore their maximum achievable upstream transmission performances on the IMDD PON systems. The results show that the DFT-spread technique in the proposed PON is effective in enhancing the upstream transmission performance to its maximum potential, whilst still maintaining all of the salient features associated with previously reported PONs. Compared with previously reported PONs excluding DFT-spread, a significant peak-to-average power ratio (PAPR) reduction of over 2 dB is achieved, leading to a 1 dB reduction in the optimal signal clipping ratio (CR). As a direct consequence of the PAPR reduction, the proposed PON has excellent tolerance to reduced digital-to-analogue converter/analogue-to-digital converter (DAC/ADC) bit resolution, and can therefore ensure the utilization of a minimum DAC/ADC resolution of only 6 bits at the forward error correction (FEC) limit (1 × 10-3). In addition, the proposed PON can improve the upstream power budget by >1.4 dB and increase the aggregate upstream signal transmission rate by up to 10% without degrading nonlinearity tolerances.

Soil water-stable aggregates and microbial community under long-term tillage in black soil of Northern China.

Long-term frequent tillage would cause black soil degradation and serious soil erosion as soil microbial communities and soil structure are extremely sensitive to tillage process. However, there is no unified conclusion on the relationship between the distribution of soil water-stable aggregates (WSAs), and microbial community construction and diversity under long-term tillage in black soil during different seasons. In this study, we used wet-sieving method to evaluate the composition and stability of soil WSAs and employed Illumina MiSeq high-throughput sequencing technology to study the diversity, taxonomic composition and co-occurrence network properties of microbial community, comparing outcomes between uncultivated soil and long-term cultivated soil for 60 years in Keshan farm of Heilongjiang Province. The results showed that after long-term tillage, the proportion of larger than 1 mm WSAs reduced by 34.17-51.37%, and the stability of WSAs, soil pH, organic matter (OM), total nitrogen (TN) contents decreased significantly in all seasons (P < 0.05), while soil available phosphorus (AP) and available potassium (AK) contents increased remarkably (P < 0.05). The diversity of bacteria increased, while that of fungi decreased. Soil fungal communities were more susceptible to long-term tillage than bacterial and archaeal communities. Actinobacteria mainly exist in large WSAs (˃1 mm), and when their relative abundance is high, it is beneficial to improve the water-stability of black soil; while Proteobacteria and Gemmatimonadetes may exist in small WSAs (˂1 mm), whose high relative abundance will weaken the water-stability of black soil. The experimental results provide a scientific theoretical basis for sustainable utilization of black soil.

Flat broadband chaos generation in a discrete-mode laser subject to optical feedback.

Chaos generation in a discrete-mode (DM) laser subject to optical feedback is experimentally explored. The results show that a DM laser with only optical feedback can produce flat broadband chaos under an optimized feedback ratio. The effect of the laser bias current on the bandwidth and flatness of chaos is also investigated. It shows that the higher bias current, the better the flatness that can be obtained at the optimal feedback ratio.

Genetic landscape and autoimmunity of monocytes in developing Vogt-Koyanagi-Harada disease.

Vogt-Koyanagi-Harada (VKH) disease is a systemic autoimmune disorder affecting multiple organs, including eyes, skin, and central nervous system. It is known that monocytes significantly contribute to the development of autoimmune disease. However, the subset heterogeneity with unique functions and signatures in human circulating monocytes and the identity of disease-specific monocytic populations remain largely unknown. Here, we employed an advanced single-cell RNA sequencing technology to systematically analyze 11,259 human circulating monocytes and genetically defined their subpopulations. We constructed a precise atlas of human blood monocytes, identified six subpopulations-including S100A12, HLA, CD16, proinflammatory, megakaryocyte-like, and NK-like monocyte subsets-and uncovered two previously unidentified subsets: HLA and megakaryocyte-like monocyte subsets. Relative to healthy individuals, cellular composition, gene expression signatures, and activation states were markedly alternated in VKH patients utilizing cell type-specific programs, especially the CD16 and proinflammatory monocyte subpopulations. Notably, we discovered a disease-relevant subgroup, proinflammatory monocytes, which showed a discriminative gene expression signature indicative of inflammation, antiviral activity, and pathologic activation, and converted into a pathologic activation state implicating the active inflammation during VKH disease. Additionally, we found the cell type-specific transcriptional signature of proinflammatory monocytes, ISG15, whose production might reflect the treatment response. Taken together, in this study, we present discoveries on accurate classification, molecular markers, and signaling pathways for VKH disease-associated monocytes. Therapeutically targeting this proinflammatory monocyte subpopulation would provide an attractive approach for treating VKH, as well as other autoimmune diseases.

NLRP12- and NLRC4-mediated corneal epithelial pyroptosis is driven by GSDMD cleavage accompanied by IL-33 processing in dry eye.

PURPOSE: Dry eye disease (DED) is a common and multifactor-induced autoimmune ocular surface disease. Environmental factors, such as desiccating stress (DS) and hyperosmolarity, affect the corneal epithelium to induce ocular surface inflammation in DED. We aimed to explore the potential mechanisms by which innate immunity and pyroptosis are initiated in the mucosal epithelium in response to environmental stress. METHODS: Experimental dry eye was established in C57BL/6 J mice and genetic mice on the background of C57BL/6 J mice by subcutaneous injection of scopolamine and exposure to a desiccating environment. SDHCEC cell line was subjected to hyperosmolarity stress (450 mOsM). The phenol red thread tear test and corneal epithelial defects evaluation were used as assessments of severity of DED. RNA-sequencing, quantitative real-time PCR, western blotting and immunofluorescence staining were performed in this study. RESULTS: Loss-of-function studies indicated that genetic deletion of GSDMD alleviates DS-induced corneal epithelium defects, and GSDMD is needed for IL-33 processing. We further found that NLRP12 collaborates with NLRC4 inflammasome to initiate GSDMD-dependent pyroptosis, which requires TLR4-induced caspase-8 (CASP8) activation in the mucosal corneal epithelium in response to DS. CONCLUSIONS: These findings provide compelling evidence that GSDMD-dependent pyroptosis plays a pivotal role in DED. A novel mechanism involving NLRP12 and NLRC4 inflammasomes-induced GSDMD-dependent pyroptosis, accompanied by IL-33 processing is responsible for ocular surface epithelial defects in response to environmental stress. GSDMD is required for IL-33 processing and the subsequent amplification of inflammatory cascades. These findings reveal novel therapeutic targets for treating DED.

NLRP12 collaborates with NLRP3 and NLRC4 to promote pyroptosis inducing ganglion cell death of acute glaucoma.

BACKGROUND: Acute glaucoma, characterized by a sudden elevation in intraocular pressure (IOP) and retinal ganglion cells (RGCs) death, is a major cause of irreversible blindness worldwide that lacks approved effective therapies, validated treatment targets and clear molecular mechanisms. We sought to explore the potential molecular mechanisms underlying the causal link between high IOP and glaucomatous RGCs death. METHODS: A murine retinal ischemia/ reperfusion (RIR) model and an in vitro oxygen and glucose deprivation/reoxygenation (OGDR) model were used to investigate the pathogenic mechanisms of acute glaucoma. RESULTS: Our findings reveal a novel mechanism of microglia-induced pyroptosis-mediated RGCs death associated with glaucomatous vision loss. Genetic deletion of gasdermin D (GSDMD), the effector of pyroptosis, markedly ameliorated the RGCs death and retinal tissue damage in acute glaucoma. Moreover, GSDMD cleavage of microglial cells was dependent on caspase-8 (CASP8)-hypoxia-inducible factor-1α (HIF-1α) signaling. Mechanistically, the newly identified nucleotide-binding leucine-rich repeat-containing receptor (NLR) family pyrin domain-containing 12 (NLRP12) collaborated with NLR family pyrin domain-containing 3 (NLRP3) and NLR family CARD domain-containing protein 4 (NLRC4) downstream of the CASP8-HIF-1α axis, to elicit pyroptotic processes and interleukin-1ß (IL-1ß) maturation through caspase-1 activation, facilitating pyroptosis and neuroinflammation in acute glaucoma. Interestingly, processing of IL-1ß in turn magnified the CASP8-HIF-1α-NLRP12/NLRP3/NLRC4-pyroptosis circuit to accelerate inflammatory cascades. CONCLUSIONS: These data not only indicate that the collaborative effects of NLRP12, NLRP3 and NLRC4 on pyroptosis are responsible for RGCs death, but also shed novel mechanistic insights into microglial pyroptosis, paving novel therapeutic avenues for the treatment of glaucoma-induced irreversible vision loss through simultaneously targeting of pyroptosis.

Numerical investigation of photonic microwave generation in an optically injected semiconductor laser subject to filtered optical feedback.

Enhanced photonic microwave generation by using a filtered optical feedback in an optically injected semiconductor laser operating at period-one (P1) dynamics is numerically demonstrated. In the simulation, the frequency tunability of the generated narrow-linewidth photonic microwave with the filtered optical feedback has been investigated. The results show that the frequency of the narrow-linewidth photonic microwave can be widely tuned by adjusting the injection parameters only or adjusting both the injection parameters and the center frequency of the filter. Moreover, the influence of the delay time, feedback strength, filter bandwidth and detuning on the linewidth, side-peak suppression and phase noise of the generated microwave have also been investigated in detail. The results show that with increasing feedback strength or delay time, evident reduction of the linewidth is observed. The side-peak suppression also increases with increasing feedback strength; however, side-peak suppression decreases with increasing feedback delay time. In addition, the linewidth reduction and side-peak suppression are relatively robust to the filter detuning, especially for higher feedback strengths and microwave frequencies. This is mainly attributed to the self-adaptive shifting of the red-shifted cavity resonance frequency to the center frequency of the filter in the FOF configuration.

Narrow-linewidth single-frequency photonic microwave generation in optically injected semiconductor lasers with filtered optical feedback.

A narrow-linewidth single-frequency photonic-microwave-generation scheme using an optically injected semiconductor laser with a filtered optical feedback has been proposed. The filtered feedback comes from a single feedback loop, which includes a narrow bandpass filter. With the filtered feedback, the linewidth of the generated microwave can be significantly reduced from 22.4 MHz to 9.0 kHz, with the side peaks suppression of 28 dB. The proposed scheme shows superior performance compared to the conventional single-feedback configuration in terms of linewidth reduction and side peaks suppression. The proposed scheme also achieves better results compared to the complex dual-feedback setting. The mechanism for a better performance of filtered optical feedback is that the filtered feedback can effectively limit the external cavity modes and stabilize the period-one dynamics. In addition, the microwave linewidth decreases with the increase of the filter width until the optimized filter width is reached. Furthermore, the linewidth reduction and the side peaks suppression of a photonic microwave using filtered optical feedback is relatively insensitive to the frequency detuning between the filter center frequency and the free-running frequency of the semiconductor laser.

TLR4-MyD88 pathway promotes the imbalanced activation of NLRP3/NLRP6 via caspase-8 stimulation after alkali burn injury.

Alkali burn (AB) is one of the most serious ocular traumas in the world, characterized by extreme ocular surface disorders, critical secondary dry eye and irreversible vision loss. The exact mechanisms involved are unknown. Innate immunity, including the involvement of Toll-like receptors (TLRs) and NOD-like receptors (NLRs), is believed to participate in the pathogenesis of the epithelia, but the exact mechanisms by which TLRs transduce signals to NLRs and downstream molecules to initiate innate immunity remain poorly defined. In this present study, we used murine models of AB and AB concomitant desiccating stress (DS) to investigate the potential functions and mechanisms of TLR4 in regulating NLRP3 and NLRP6 during AB injury and secondary dry eye. We demonstrated that AB injury induced activation of the TLR4-MyD88 pathway, leading to imbalanced NLRP3 and NLRP6 via the activation of caspase-8 signaling. DS worsened ocular surface disorders post-AB injury by magnifying this phenomenon. Caspase-8 signaling promoted NLRP3 upregulation via the nuclear factor (NF)-κB pathway, while NLRP6 suppressed NF-κB activation. Our findings also revealed that TLR4-MyD88 knockout can alleviate AB-induced or DS-worsened ocular surface disorders, shedding light on the potential therapeutic strategies in the future for AB injury. Taken together, our findings demonstrate that AB promotes the TLR4-MyD88-caspase-8 axis to cause imbalanced NLRP3/NLRP6, and DS exacerbates ocular surface damage via magnifying this imbalance.

Broad tunable photonic microwave generation based on period-one dynamics of optical injection vertical-cavity surface-emitting lasers.

Photonic microwave generation based on period-one dynamics of an optically injected VCSEL has been study experimentally. The results have shown that the frequency of the generated microwave signal can be broadly tunable through the adjustment of the injection power and the frequency detuning. Strong optical injection power and higher frequency detuning are favorable for obtaining a high frequency microwave signal. These results are similar to those found in systems based on distributed feedback lasers and quantum dot lasers. The variation of the microwave power at the fundamental frequency and the second-harmonic distortion have also been characterized.

Effect of digital acquisition on the complexity of chaos.

The effect of data acquisition on the complexity of chaos using permutation entropy has been studied experimentally and numerically. The complexity of chaos is quantified using the normalized permutation entropy at the feedback round-trip time. Two "abnormal" variations of the complexity of chaos with bias current have been observed experimentally. The different vertical scales of the oscilloscope in data acquisition are attributed to this abnormal change. The method to remove the effect of data acquisition has also been proposed.