Hemispheric asymmetry in ocean change and the productivity of ecosystem sentinels.

Climate change and other human activities are causing profound effects on marine ecosystem productivity. We show that the breeding success of seabirds is tracking hemispheric differences in ocean warming and human impacts, with the strongest effects on fish-eating, surface-foraging species in the north. Hemispheric asymmetry suggests the need for ocean management at hemispheric scales. For the north, tactical, climate-based recovery plans for forage fish resources are needed to recover seabird breeding productivity. In the south, lower-magnitude change in seabird productivity presents opportunities for strategic management approaches such as large marine protected areas to sustain food webs and maintain predator productivity. Global monitoring of seabird productivity enables the detection of ecosystem change in remote regions and contributes to our understanding of marine climate impacts on ecosystems.

Rapid regulation of dopamine transporters by tyrosine kinases in rat neuronal preparations.

Termination of dopamine neurotransmission is primarily controlled by the plasma membrane-localized dopamine transporter. In this study, we investigated how this transporter is regulated by tyrosine kinases in neuronal preparations. In rat dorsal striatal synaptosomes, inhibition of tyrosine kinases by genistein or tyrphostin 23 resulted in a rapid (5-15 min), concentration-dependent decrease in [(3)H]dopamine uptake because of a reduction in maximal [(3)H]dopamine uptake velocity and dopamine transporter cell surface expression. The reduced transporter activity was associated with a decrease in phosphorylated p44/p42 mitogen-activated protein kinases. In primary rat mesencephalic neuronal cultures, the tyrosine kinase inhibitors similarly reduced [(3)H]dopamine uptake. When cultures were serum-deprived, acute activation of tyrosine kinase-coupled TrkB receptors by 100 ng/mL brain-derived neurotrophic factor significantly increased [(3)H]dopamine uptake; the effects were complex with increased maximal velocity but reduced affinity. The facilitatory effect of brain-derived neurotrophic factor on dopamine transporter activity depended on both the mitogen-activated protein kinase and phosphatidylinositol 3-kinase pathways. Taken together, our results suggest that striatal dopamine transporter function and cell surface expression is constitutively up-regulated by tyrosine kinase activation and that brain-derived neurotrophic factor can mediate this type of rapid regulation.

Rapid word-learning in normal-hearing and hearing-impaired children: effects of age, receptive vocabulary, and high-frequency amplification.

OBJECTIVE: This study examined rapid word-learning in 5- to 14-year-old children with normal and impaired hearing. The effects of age and receptive vocabulary were examined as well as those of high-frequency amplification. Novel words were low-pass filtered at 4 kHz (typical of current amplification devices) and at 9 kHz. It was hypothesized that (1) the children with normal hearing would learn more words than the children with hearing loss, (2) word-learning would increase with age and receptive vocabulary for both groups, and (3) both groups would benefit from a broader frequency bandwidth. DESIGN: Sixty children with normal hearing and 37 children with moderate sensorineural hearing losses participated in this study. Each child viewed a 4-minute animated slideshow containing 8 nonsense words created using the 24 English consonant phonemes (3 consonants per word). Each word was repeated 3 times. Half of the 8 words were low-pass filtered at 4 kHz and half were filtered at 9 kHz. After viewing the story twice, each child was asked to identify the words from among pictures in the slide show. Before testing, a measure of current receptive vocabulary was obtained using the Peabody Picture Vocabulary Test (PPVT-III). RESULTS: The PPVT-III scores of the hearing-impaired children were consistently poorer than those of the normal-hearing children across the age range tested. A similar pattern of results was observed for word-learning in that the performance of the hearing-impaired children was significantly poorer than that of the normal-hearing children. Further analysis of the PPVT and word-learning scores suggested that although word-learning was reduced in the hearing-impaired children, their performance was consistent with their receptive vocabularies. Additionally, no correlation was found between overall performance and the age of identification, age of amplification, or years of amplification in the children with hearing loss. Results also revealed a small increase in performance for both groups in the extended bandwidth condition but the difference was not significant at the traditional p = 0.05 level. CONCLUSIONS: The ability to learn words rapidly appears to be poorer in children with hearing loss over a wide range of ages. These results coincide with the consistently poorer receptive vocabularies for these children. Neither the word-learning or receptive-vocabulary measures were related to the amplification histories of these children. Finally, providing an extended high-frequency bandwidth did not significantly improve rapid word-learning for either group with these stimuli.

Overview of the reanalysis of the Harvard Six Cities Study and American Cancer Society Study of Particulate Air Pollution and Mortality.

This article provides an overview of the Reanalysis Study of the Harvard Six Cities and the American Cancer Society (ACS) studies of particulate air pollution and mortality. The previous findings of the studies have been subject to debate. In response, a reanalysis team, comprised of Canadian and American researchers, was invited to participate in an independent reanalysis project to address the concerns. Phase I of the reanalysis involved the design of data audits to determine whether each study conformed to the consistency and accuracy of their data. Phase II of the reanalysis involved conducting a series of comprehensive analyses using alternative statistical methods. Alternative models were also used to identify covariates that may confound or modify the association of particulate air pollution as well as identify sensitive population subgroups. The audit demonstrated that the data in the original analyses were of high quality, as were the risk estimates reported by the original investigators. The sensitivity analysis illustrated that the mortality risk estimates reported in both studies were found to be robust against alternative Cox models. Detailed investigation of the covariate effects found a significant modifying effect of education and a relative risk of mortality associated with fine particles and declining education levels. The study team applied spatial analytic methods to the ACS data, resulting in various levels of spatial autocorrelations supporting the reported association for fine particles mortality of the original investigators as well as demonstrating a significant association between sulfur dioxide and mortality. Collectively, our reanalysis suggest that mortality may be attributable to more than one component of the complex mixture of ambient air pollutants for U.S. urban areas.

Retrospective data quality audits of the Harvard Six Cities and American Cancer Society studies.

The Harvard Six Cities (6-Cities) and American Cancer Society (ACS) studies are longitudinal cohort mortality studies of large populations that provided important information about the human health effects associated with long-term exposure to fine particulate air pollution. Possible changes to federal regulation of particulates prompted a review of data collection methods, analysis, and reported results from these two studies. This article describes the methodology used to conduct quality assurance audits of both studies and summarizes the audit findings. Statistically based, randomly selected samples of 250 health questionnaires and 250 death certificates from each study were audited against data from analysis files. In cases where study-specific data could not be located, validation was performed using information and data from other sources. Some errors were found in programming and data transformation in both studies, but none affected the results of the original investigations. Both audits confirmed that the published studies are an accurate representation of the collected data. The audits also underscored the importance of adequate attention to documentation and record-keeping practices during the conduct of all studies and proper archiving at their conclusion.

Further characterization of preproenkephalin mRNA-containing cells in the rodent globus pallidus.

The globus pallidus (external pallidum of primates) is an essential nucleus within basal ganglia circuitry, in part because it receives at least one-half of striatal efferent projections. Neurons of the globus pallidus can be divided into subpopulations based on anatomical, physiological, and chemical features. Globus pallidus neurons project to several structures (the striatum, subthalamic nucleus, entopeduncular nucleus, and substantia nigra pars reticulata), have one of two alternative waveforms (positive/negative versus negative/positive), contain either the calcium binding protein parvalbumin or the neuropeptide precursor preproenkephalin mRNA and show differential immediate early gene responses to dopamine receptor agonists and antagonists. The objective of the present study was to characterize in greater detail the preproenkephalin mRNA-containing pallidal neurons using Sprague-Dawley rats. In situ hybridization for preproenkephalin mRNA was combined with immunocytochemical detection of: (i) the neuron-specific nuclear protein, NeuN, (ii) FluoroGold-labeled pallidostriatal and pallidosubthalamic cells, or (iii) Fos induced by either systemic combined D1-class/D2-class dopamine receptor agonists or a D2-class receptor antagonist. These experiments demonstrated that a substantial population (42%) of globus pallidus neurons contains preproenkephalin mRNA, and that globus pallidus neurons retrogradely labeled after FluoroGold injections into the striatum are more frequently preproenkephalinergic, compared to the population of pallidosubthalamic neurons. Furthermore, systemic administration of a D2 receptor antagonist, eticlopride, induced Fos immunoreactivity predominantly in globus pallidus neurons expressing preproenkephalin mRNA, while combined administration of D1 and D2 receptor agonists induced Fos predominantly in pallidal neurons lacking preproenkephalin mRNA.These results support the conclusion that preproenkephalin mRNA identifies one of the two major subpopulations of pallidal neurons. This preproenkephalin mRNA-expressing pallidal subpopulation preferentially targets the striatum and is more readily activated in its immediate early gene expression by D2 receptor antagonists than by dopamine receptor agonists. This projection provides a pallidal substrate for the dopaminergic regulation of striatal information processing.

Effect of stimulus bandwidth on the perception of /s/ in normal- and hearing-impaired children and adults.

Recent studies with adults have suggested that amplification at 4 kHz and above fails to improve speech recognition and may even degrade performance when high-frequency thresholds exceed 50-60 dB HL. This study examined the extent to which high frequencies can provide useful information for fricative perception for normal-hearing and hearing-impaired children and adults. Eighty subjects (20 per group) participated. Nonsense syllables containing the phonemes /s/, /f/, and /O/, produced by a male, female, and child talker, were low-pass filtered at 2, 3, 4, 5, 6, and 9 kHz. Frequency shaping was provided for the hearing-impaired subjects only. Results revealed significant differences in recognition between the four groups of subjects. Specifically, both groups of children performed more poorly than their adult counterparts at similar bandwidths. Likewise, both hearing-impaired groups performed more poorly than their normal-hearing counterparts. In addition, significant talker effects for /s/ were observed. For the male talker, optimum performance was reached at a bandwidth of approximately 4-5 kHz, whereas optimum performance for the female and child talkers did not occur until a bandwidth of 9 kHz.

The role of the globus pallidus D2 subfamily of dopamine receptors in pallidal immediate early gene expression.

The globus pallidus plays an important role in basal ganglia circuitry, representing the first relay nucleus of the 'indirect pathway' of striatal efferents. In contrast to the well-characterized actions of dopamine on striatal neurons, the functional role of the dopamine innervation of globus pallidus is less well understood. Previous research showed that systemic administration of either a dopamine D2 receptor antagonist or combined dopamine D1 and D2 receptor agonists induces Fos, the protein product of the immediate early gene c-fos, in neurons of globus pallidus [Ruskin and Marshall (1997) Neuroscience 81, 79-92]. To determine whether the ability of the D2 receptor antagonist, sulpiride, to induce Fos in rat pallidal neurons is mediated by D2-like receptors in striatum or globus pallidus, intrastriatal or intrapallidal sulpiride infusions were conducted. The diffusion of intrastriatal sulpiride was estimated by measuring this antagonist's competition for N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ)-induced D2 receptor inactivation. The phenotype of the striatal neurons expressing Fos after intrastriatal infusion was assessed by combining Fos immunocytochemistry with D2 receptor mRNA in situ hybridization. Intrastriatal infusions of (-)-sulpiride (10-200 ng) dose-dependently increased the number of striatal cells expressing Fos; and the Fos-immunoreactive striatal cells were D2 receptor mRNA-expressing, the same population in which systemic D2 receptor antagonists induce Fos. Intrastriatal infusions of high (5 microg), but not low (10-200 ng), (-)-sulpiride doses also induced Fos in globus pallidus cells but the sulpiride appeared to spread to the globus pallidus. Direct intrapallidal infusions of (-)-sulpiride (50-100 ng) dose-dependently induced Fos in globus pallidus with minimal influence on striatum or other basal ganglia structures. Using sensitive in situ hybridization conditions, prominent labeling of D2 receptor mRNA was evident in globus pallidus. D2 receptor mRNA was densest in a lateral 200 microm wide band that follows the curvature of the pallidal/striatal boundary. Cellular analysis revealed silver clusters associated with D2 receptor mRNA labeling over globus pallidus neurons that were immunoreactive for neuron-specific nuclear protein. These results strongly suggest that the dopaminergic innervation of globus pallidus, acting through D2-like receptors internal to this structure, can control gene expression in pallidal neurons.

Some issues relevant to establishing a universal newborn hearing screening program.

This article describes some of the factors relevant to the establishment of a universal newborn hearing screening (UNHS) program. First, the difficulty in providing precise estimates of test sensitivity and specificity are reviewed. This section is followed by hypothetical estimates of overall programmatic costs, first for a fixed number of babies to be screened and then as a function of the number of babies to be screened in a year. Included in these estimates are the costs for equipment, disposables, personnel, and follow-up testing. These estimates are provided for three different screening protocols: auditory brainstem response (ABR) alone, otoacoustic emission (OAE) alone, and OAE followed by ABR only for those babies who failed the OAE screening. If follow-up costs are not included, it is less expensive to screen newborns with OAEs compared with the other two protocols. However, once follow-up testing is included as part of the program costs and there are at least 400 births per year, procedures in which OAEs are performed first, followed by an ABR on those infants who do not pass the OAE test, result in the lowest costs. Hospitals with as few as 400 births per year should expect per-baby costs not exceeding $30, regardless of which protocol is used. For all three protocols, the unit costs decrease as the number of babies screened increases. The final section describes data from a local UNHS program in which all infants are screened first with an OAE test, followed by an ABR test on infants not passing the OAE screening. Idiosyncratic features to this program are described, including the fact that all screening tests are performed by audiologists, who are paid on a part-time basis, adding cost to the program. Even under these circumstances, the unit cost is under $30. These data lead us to conclude that all infants can be screened in a cost-effective manner.

Ultrafast, cross-correlated harmonic imaging through scattering media.

A simple upconversion scheme utilizing 40-fs pulses is shown to permit high-contrast imaging of objects obscured by a highly scattering medium when no ballistic component is evident in the scattered light and imaging is performed with any portion of the scattered light pulse. We present a time-gated, inherently low-pass spatially filtered imaging method that minimizes signal-averaging requirements and greatly facilitates imaging under severe scattering (turbid) conditions.

Investigation of ultrafast time gating by spatial filtering.

With a spatial-filtering method of gating, we explore image formation through scattering media using first-arriving light. Gating times of a few femtoseconds and less are produced, and the resolution at these extremely short gating times is investigated.

Effect of earmold fit on predicted real ear SPL using a real ear to coupler difference procedure.

OBJECTIVES: The goal of Experiment I was to quantify the SPL entering the ear canal via a secondary pathway created by a vent in the earmold and/or a slit leak around the earmold. The goal of Experiment II was to determine the validity of a real ear to coupler difference (RECD) procedure under conditions that are likely to produce errors (e.g., when hearing aid gain in the low frequencies is minimal and large negative RECD values occur as a result of venting or a loosely fitting earmold). DESIGN: In Experiment I, the SPL entering the ear via the secondary pathway was measured in 61 hearing-impaired children and 13 normal-hearing adults. In Experiment II, traditional probe microphone measures of real ear SPL were compared to the SPL predicted using the RECD procedure in five normal-hearing adults with loosely fitting earmolds. RESULTS: Results of Experiment I indicated that sound entered the ear canal unattenuated at 250 and 500 Hz, regardless of earmold fit, vent size, or subject age. In Experiment II, the largest differences between traditional probe microphone measures of SPL and predicted measures were noted when hearing aid gain was 0 dB and large negative RECD values were present. When hearing aid gain was minimal and the RECD was in the -10 to -22 dB range, predicted values underestimated the real ear SPL by an average of 14 dB. CONCLUSIONS: Although the results of this study apply only to a limited range of conditions found in clinical practice, in those cases, the errors may influence clinical decisions about the type of hearing aid fitted and the amount of gain provided. Potential solutions to this problem are discussed.

The relation between stimulus context, speech audibility, and perception for normal-hearing and hearing-impaired children.

In this study, the influence of stimulus context and audibility on sentence recognition was assessed in 60 normal-hearing children, 23 hearing-impaired children, and 20 normal-hearing adults. Performance-intensity (PI) functions were obtained for 60 semantically correct and 60 semantically anomalous sentences. For each participant, an audibility index (AI) was calculated at each presentation level, and a logistic function was fitted to rau-transformed percent-correct values to estimate the SPL and AI required to achieve 70% performance. For both types of sentences, there was a systematic age-related shift in the PI functions, suggesting that young children require a higher AI to achieve performance equivalent to that of adults. Improvement in performance with the addition of semantic context was statistically significant only for the normal-hearing 5-year-olds and adults. Data from the hearing-impaired children showed age-related trends that were similar to those of the normal-hearing children, with the majority of individual data falling within the 5th and 95th percentile of normal. The implications of these findings in terms of hearing-aid fitting strategies for young children are discussed.

Comparison of field correlations in multiply scattered quasi-monochromatic light.

An experiment is described that directly compares the degradations, with the number of scattering mean free paths, of two field correlations that may be used to form gates for imaging techniques in scattered light: the correlation of the scattered wave with an unscattered reference wave and the correlation of two wave-vector components of the scattered wave itself. Results for 20-microm polymer spheres show that the latter correlation is consistently larger well into the multiple-scattering regime (up to 10 mean free paths) for wave-vector separations less than at least 50 mm(-1) and that the two correlations tend to merge in this scattering regime for larger wave-vector separations.

Recognition performance for four combinations of FM system and hearing aid microphone signals in adverse listening conditions.

OBJECTIVE: Children with moderate to severe hearing loss routinely use personal frequency modulated (FM) systems in the classroom to improve the signal to noise ratio of teacher-directed speech with notable success. Attention is now being given to the ability of these children to hear other students via the hearing aid (HA) microphone while using an FM system. As a result, a variety of FM system and HA microphone combinations have been recommended for classroom use. To date, there are no studies regarding the efficacy of these FM/HA combinations. The purpose of this study was to evaluate recognition performance using four FM/HA combinations and to characterize that performance for stimuli received primarily through FM system and HA microphone transmission. DESIGN: Recognition performance for FM system and HA microphone signals was evaluated for two symmetrical and two asymmetrical FM/HA combinations using two commercially available FM systems (one conventional and one FM-precedence circuit). Eleven children (ages 9 to 12) with moderate to severe sensorineural hearing loss and eight children (ages 10 to 11) with normal hearing served as subjects. The two symmetrical FM/HA combinations included: 1) binaural FM system and HA microphone input using the conventional FM system, and 2) binaural FM and HA input using the FM-precedence circuit. The conventional FM system was used for the two asymmetrical combinations and included: 1) binaural FM input and monaural HA input, and 2) FM input to one ear and HA input to the other. Stimuli were 33 consonants presented in the form of nonsense syllables. The stimuli were presented through three loudspeakers representing a teacher and two fellow students in a classroom environment. Speech shaped noise was presented through two additional loudspeakers. RESULTS: In general, no statistically significant differences in recognition performance were found between any of the FM/HA combinations. Mean recognition scores for HA microphone transmission (55%) were significantly poorer than those for FM system transmission (75%). As expected, initial consonants were more easily recognized than final consonants via FM system and HA microphone transmission. However, voiceless consonants were more easily recognized than voiced consonants via HA microphone transmission, which was not predicted on the basis of previous research. CONCLUSIONS: These results suggest that a certain amount of flexibility is present when choosing an FM/HA combination. However, recognition performance via the HA microphones was consistently poorer than performance via FM transmission. Because relevant material also originates from fellow students (e.g., answering teacher-directed questions), input via the HAs is often as important as information originating from the teacher. The results suggest that attempts to improve performance for signals transmitted through the HA microphones in a classroom setting would benefit children with hearing loss.

Population characteristics of preproenkephalin mRNA-containing neurons in the globus pallidus of the rat.

Anatomical, neurochemical and electrophysiological evidence indicates the presence of multiple neuronal subpopulations within the rodent globus pallidus (GP). One subpopulation that has not been well characterized is GP neurons that express preproenkephalin mRNA (PPE+ cells). The present study seeks to further characterize GP subpopulations by determining whether the PPE+ GP neurons express parvalbumin immunoreactivity (PV-IR) and where their axons project by retrogradely labeling pallidal neurons with the tracer FluoroGold (FG). Using combined PV immunocytochemistry (ICC) and PPE mRNA in situ hybridization, we observed that PV-IR and PPE mRNA identify predominantly separate pallidal cell populations. Combined FG ICC and PPE mRNA in situ hybridization also revealed that this neuropeptide mRNA is more often found in FG-labeled pallidostriatal than pallidosubthalamic neurons. Our data support a growing body of evidence that suggests the GP is more heterogeneous than accounted for by current functional models of the basal ganglia.

Subjective effects of peak clipping and compression limiting in normal and hearing-impaired children and adults.

Despite many advances in hearing-aid signal processing, compression limiting and peak clipping are still used. To date, perceptual studies have been conducted only with adults. The current study was designed to investigate the clarity of peak-clipped and compressed speech for both adults and children. Subjects were 30 normal-hearing and 30 hearing-impaired individuals in three age ranges (7-9, 10-12, and 16-50 years). Stimuli were processed at 60, 70, 75, and 80 dB SPL using peak clipping and at 80 dB SPL using compression limiting. Paired-comparison measures were used to assess the clarity of sentences, and a signal-to-distortion ratio (SDR) based on a measure of coherence between input and output was computed for each condition. For the peak-clipping conditions, there was a decrease in perceived clarity as the input increased from 60 to 80 dB SPL. This perceptual continuum was most apparent for the normal-hearing adults. The normal-hearing 10-12 year olds and the hearing-impaired adults showed a similar, but less pronounced, pattern. In contrast, the remaining three subject groups showed minimal differences in perceived clarity across conditions. Surprisingly, only the two oldest normal-hearing groups showed a clear preference for compression limiting over peak clipping at the highest input level, and only their results were consistent with the pattern of coherence across stimuli. Judgments of clarity by the normal-hearing subjects correlated best with the SDR in the 500-2000-Hz range, while clarity judgments of the hearing-impaired subjects correlated best with the SDR below 1000 Hz.

Realization of time gating by use of spatial filtering.

A method for simulating conventional time gating in low-coherence optical imaging processes in highly scattering media is given. The method uses monochromatic instead of broadband light, and spatial filtering is substituted for time gating. The process enables the study of imaging techniques in scattering media to be carried out in an easy and highly controllable way. Experimental results are given.

A comparison of threshold-based fitting strategies for nonlinear hearing aids.

OBJECTIVE: In recent years, wide dynamic range compression (WDRC) has been used with increasing success. To optimize the fit with this type of hearing aid circuitry, subjective measures of loudness growth often are used. Unfortunately, these type of measures cannot be performed with infants, young children, and some elderly individuals. The primary purpose of this study was to compare the fitting recommendations of two recently described threshold-based procedures for fitting nonlinear hearing aids (DSL 4.0 and FIG6) to the use gain settings of satisfied adult hearing aid users for whom the fitting was based on subjective measures of loudness growth. Because it cannot be assumed that the use settings for adults will be appropriate for young children, a secondary goal was to quantify the audibility of speech at the use settings derived from loudness growth measures. DESIGN: Forty-nine adult hearing aid users with mild to severe sensorineural hearing loss participated in this study. For all subjects, loudness growth measures were used to optimize the fit of a 2-channel WDRC hearing aid. The use gain at 50 and 80 dB SPL was compared with the gain recommended by DSL, FIG6, and the manufacturer's threshold-based fitting algorithm. RESULTS: In general, both DSL and FIG6 prescribed more gain than actually was used by these hearing aid wearers. These discrepancies increased as a function of frequency, and differences in excess of 20 dB were observed in some cases. The manufacturer's algorithm provided a closer approximation to the use gain than either DSL or FIG6. Utilizing these use gain values, an Aided Audibility Index (AAI) was calculated for soft, average, and loud speech across four degrees of hearing loss, ranging from mild to severe (12 conditions). Transfer functions for continuous discourse and nonsense syllables were applied to yield estimated intelligibility scores. For the higher context speech materials, estimated intelligibility was > or = 85% for nine of the 12 conditions. For low-context speech materials, estimated intelligibility was > or = 85% for only three of the 12 conditions. CONCLUSIONS: Results suggest that the gain recommendations provided by both DSL and FIG6 exceeded the gain actually used by the adult hearing-impaired subjects in this study. Gain recommendations from the manufacturer's algorithm provided a closer approximation to the use gain values of these subjects. These findings suggest that, for adult hearing aid users who cannot perform loudness judgments reliably, the manufacturer's algorithm would be expected to provide a closer approximation to loudness-based use gain values than either DSL or FIG6. However, AAI calculations revealed that the gain recommendations from this algorithm produce adequate audibility of speech only if one assumes linguistic competence. When AAI values are transformed to predict the intelligibility of low-context speech materials, it appears that the degree of audibility may not be appropriate for prelingually hearing-impaired children with more than a moderate hearing loss.