1.
J Am Mosq Control Assoc
; 29(4): 376-9, 2013 Dec.
Article
in English
| MEDLINE
| ID: mdl-24551971
ABSTRACT
Using novel DNA sequence data, we designed a restriction enzyme assay that distinguishes Aedes atlanticus and Ae. tormentor, based on size polymorphisms. The restriction endonuclease Hpy188I digests polymerase chain reaction-amplified 2nd internal transcribed spacer products once for Ae. atlanticus and twice for Ae. tormentor, thus providing a useful method for identifying adult female collections that are generally considered morphologically indistinguishable.