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1.
J Pharmacol Sci ; 147(1): 81-85, 2021 Sep.
Article in English | MEDLINE | ID: mdl-34294376

ABSTRACT

Pulmonary arterial hypertension (PAH) is a rare, progressive, and fatal cardiovascular/lung disease. The incidence rate is affected by age. Monocrotaline (MCT, 60 mg/kg)-treated rats are widely used as an experimental PAH model. Here, we found that young rats died at a mean of 23.4 days after MCT injection, whereas adult rats survived for over 42 days. However, young (7-week-old) and adult (20-week-old) MCT-treated rats developed PAH, and had upregulated Ca2+-sensing receptor and transient receptor potential canonical subfamily 6 channel expression in pulmonary arteries. The present study provides novel information for elucidating the mechanism underlying the age difference in PAH patients.


Subject(s)
Hypertension, Pulmonary/metabolism , Monocrotaline/adverse effects , Adult , Age Factors , Animals , Calcium Channels/metabolism , Disease Models, Animal , Female , Humans , Hypertension, Pulmonary/chemically induced , Male , Middle Aged , Pulmonary Artery/metabolism , Rats, Sprague-Dawley , Receptors, Calcium-Sensing/metabolism
2.
Head Neck ; 36(3): 411-8, 2014 Mar.
Article in English | MEDLINE | ID: mdl-23728900

ABSTRACT

BACKGROUND: Radioresistance remains a critical issue in the use of radiotherapy for the treatment of head and neck squamous cell carcinoma (HNSCC). This study evaluated the efficacy of combination treatment with OBP-301, a telomerase-specific replication-selective adenovirus, and radiotherapy in overcoming radioresistance by examining its effect on radiation-resistant HNSCC cells. METHODS: Radiation-resistant HNSCC cells were treated with OBP-301 and radiation in vitro and in an orthotopic nude mouse model in vivo and synergism was assessed. Apoptosis and expression of MRN complex, which plays a key role in DNA repair machinery, were also analyzed. RESULTS: Infection with OBP-301 was found to enhance the antitumor efficacy of radiation both in vitro and in vivo by inhibiting MRN complex expression and increasing apoptosis induction. CONCLUSION: Combined OBP-301 and radiation therapy seems to overcome radioresistance in HNSCC cells by inhibiting DNA repair machinery, and may thus be a novel therapeutic strategy for treating HNSCC.


Subject(s)
Adenoviridae/metabolism , Carcinoma, Squamous Cell/therapy , Head and Neck Neoplasms/therapy , Oncolytic Virotherapy/methods , Telomerase/metabolism , Adenoviridae/genetics , Animals , Blotting, Western , Carcinoma, Squamous Cell/radiotherapy , DNA Repair/physiology , DNA Repair/radiation effects , Disease Models, Animal , Female , Head and Neck Neoplasms/radiotherapy , Humans , In Vitro Techniques , Mice , Mice, Inbred BALB C , Mice, Nude , Neoplastic Cells, Circulating , Radiation, Ionizing , Radiotherapy Dosage , Squamous Cell Carcinoma of Head and Neck , Telomerase/genetics , Xenograft Model Antitumor Assays
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