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1.
J Appl Microbiol ; 125(1): 142-147, 2018 Jul.
Article in English | MEDLINE | ID: mdl-29495109

ABSTRACT

AIMS: To investigate the transmission route of Carnobacterium from the farm environment to the meat-manufacturing plant and potential risk for meat spoilage. METHODS AND RESULTS: A sheep farm-level survey of Carnobacterium, consisting of 150 environmental and animal (no 100) associated samples, was carried out on two farms. A further 20 lamb carcass samples were taken from an abattoir servicing one of the farms. The majority of Carnobacterium maltaromaticum isolates were associated with fleece followed by hard sheep contact surfaces, rectal-anal mucosal swabs and carcasses. Enterobacterial repetitive intergenic consenus PCR (ERIC-PCR) profiling revealed four distinct ERIC types. Each ERIC type was found on both farms, three of which were also found on lamb carcasses. CONCLUSIONS: Enterobacterial repetitive intergenic consenus PCR was effective at demonstrating within-species variability in C. maltaromaticum. This study provides initial information showing that farm sources maybe an important transmission route of Carnobacterium for contamination of lamb carcasses and subsequently the meat processing environment. SIGNIFICANCE AND IMPACT OF THE STUDY: Data on distribution, diversity, sources and transmission routes for meat product contamination is limited for spoilage bacteria. This study highlights the importance of good hygienic slaughter practices and cleaning routines to remove accumulated detritus from the handling of animals that may lead to cross-contamination.


Subject(s)
Abattoirs , Carnobacterium/isolation & purification , Farms , Meat/microbiology , Sheep , Animals , Food Microbiology
2.
J Neurol Sci ; 293(1-2): 65-7, 2010 Jun 15.
Article in English | MEDLINE | ID: mdl-20381072

ABSTRACT

BACKGROUND: Selective serotonin re-uptake inhibitors (SSRIs) may have therapeutic potential in the treatment of ischemic stroke by effects on neuronal cell survival and the plasticity of brain processes. In the present study, we investigated whether prior treatment with a SSRI is associated with more favorable functional outcome in a cohort of patients with acute ischemic stroke treated with tissue plasminogen activator (tPA). METHODS: In a prospective observational cohort study of 476 acute ischemic stroke patients treated with tPA we investigated the relationship between prior SSRI treatment and functional outcome at 3 months. Ischemic stroke subtypes were defined according to the Oxfordshire Community Stroke Project Classification. Favorable outcome was defined as a modified Rankin Scale score

Subject(s)
Selective Serotonin Reuptake Inhibitors/therapeutic use , Stroke/drug therapy , Stroke/physiopathology , Aged , Aged, 80 and over , Female , Fibrinolytic Agents/therapeutic use , Humans , Male , Middle Aged , Multivariate Analysis , Prospective Studies , Retrospective Studies , Severity of Illness Index , Statistics, Nonparametric , Stroke/classification , Tissue Plasminogen Activator/therapeutic use
3.
Neuroscience ; 110(3): 489-504, 2002.
Article in English | MEDLINE | ID: mdl-11906788

ABSTRACT

Estradiol exerts beneficial effects on neurodegenerative disorders associated with the decline of cognitive performance. The present study was designed to further investigate the effect of 17beta-estradiol on learning and memory, and to evaluate its neuroprotective action on cholinergic cells of the nucleus basalis magnocellularis, a neural substrate of cognitive performance. Female rats were ovariectomized at an age of 6 months. Three weeks later they received injections of either a mid-physiological dose of 17beta-estradiol or vehicle (oil), every other day for 2 weeks. The effect of estradiol on cognitive performance was tested in two associative learning paradigms. In the two-way active shock avoidance task estradiol-replaced animals learned significantly faster, while in the passive shock avoidance test no differences were observed between the experimental groups. Subsequent unilateral infusion of N-methyl-D-aspartate in the nucleus basalis magnocellularis resulted in a significant loss of cholinergic neurons concomitant with the loss of their fibers invading the somatosensory cortex. Estradiol treatment did not affect the total number of choline-acetyltransferase-immunoreactive neurons and their coexpression of the p75 low-affinity neurotrophin receptor either contralateral or ipsilateral to the lesion. In contrast, cholinergic fiber densities in estradiol-treated animals were greater both in the contralateral and ipsilateral somatosensory cortices as was detected by quantitative choline-acetyltransferase and vesicular acetylcholine transporter immunocytochemistry. However, estradiol treatment did not affect the lesion-induced relative percentage loss of cholinergic fibers. A significant decline of synaptophysin immunoreactivity paralleled the cholinergic damage in the somatosensory cortex of oil-treated animals, whereas an almost complete preservation of synaptic density was determined in estradiol-treated rats. Our results indicate that estradiol treatment enhances the cortical cholinergic innervation but has no rescuing effect on cholinergic nerve cells in the basal forebrain against excitotoxic damage. Nevertheless, estradiol may restore or maintain synaptic density in the cerebral cortex following cholinergic fiber loss. This estradiol effect may outweigh the lack of cellular protection on cholinergic cells at the functional level.


Subject(s)
Basal Nucleus of Meynert/drug effects , Cerebral Cortex/drug effects , Cholinergic Fibers/drug effects , Estradiol/pharmacology , Membrane Transport Proteins , Memory/drug effects , Neuroprotective Agents/pharmacology , Presynaptic Terminals/drug effects , Vesicular Transport Proteins , Acetylcholinesterase/metabolism , Animals , Avoidance Learning/drug effects , Avoidance Learning/physiology , Basal Nucleus of Meynert/metabolism , Basal Nucleus of Meynert/pathology , Carrier Proteins/metabolism , Cerebral Cortex/metabolism , Cerebral Cortex/pathology , Choline O-Acetyltransferase/metabolism , Cholinergic Fibers/metabolism , Cholinergic Fibers/pathology , Cognition Disorders/drug therapy , Cognition Disorders/metabolism , Cognition Disorders/physiopathology , Estradiol/metabolism , Female , Immunohistochemistry , Memory/physiology , Neurodegenerative Diseases/drug therapy , Neurodegenerative Diseases/metabolism , Neurodegenerative Diseases/physiopathology , Neurotoxins/pharmacology , Ovariectomy , Presynaptic Terminals/metabolism , Presynaptic Terminals/pathology , Rats , Rats, Wistar , Receptor, Nerve Growth Factor/metabolism , Synaptophysin/metabolism , Vesicular Acetylcholine Transport Proteins
4.
Neuroscience ; 108(4): 611-27, 2001.
Article in English | MEDLINE | ID: mdl-11738498

ABSTRACT

Cholinergic neurons of the basal forebrain form one of the neuron populations that are susceptible to excitotoxic injury. Whereas neuropharmacological studies have aimed at rescuing cholinergic neurons from acute excitotoxic attacks, the short-term temporal profile of excitotoxic damage to cholinergic nerve cells remains largely elusive. The effects of N-methyl-D-aspartate (NMDA) infusion on cytochemical markers of cholinergic neurons in rat magnocellular nucleus basalis were therefore determined 4, 24 and 48 h post-lesion. Additionally, the influence of excitotoxic damage on the efficacy of in vivo labelling of cholinergic neurons with carbocyanine 3-192IgG was investigated. Carbocyanine 3-192IgG was unilaterally injected in the lateral ventricle. Twenty-four hours later, NMDA (60 nM/microl) was infused in the right magnocellular nucleus basalis, while control lesions were performed contralaterally. Triple immunofluorescence labelling for carbocyanine 3-192IgG, NMDA receptor 2A and B subunits and choline-acetyltransferase (ChAT) was employed to determine temporal changes in NMDA receptor immunoreactivity on cholinergic neurons. The extent of neuronal degeneration was studied by staining with Fluoro-Jade. Moreover, changes in the numbers of ChAT or p75 low-affinity neurotrophin receptor immunoreactive neurons, and the degree of their co-labelling with carbocyanine 3-192IgG were determined in basal forebrain nuclei. The effects of NMDA-induced lesions on cortical projections of cholinergic nucleus basalis neurons were studied by acetylcholinesterase (AChE) histochemistry. Characteristic signs of cellular damage, as indicated by decreased immunoreactivity for NMDA receptors, ChAT and p75 low-affinity neurotrophin receptors, were already detected at the shortest post-lesion interval investigated. Fluoro-Jade at 4 h post-lesion only labelled the core of the excitotoxic lesion. Longer survival led to enhanced Fluoro-Jade staining, and to the decline of ChAT immunoreactivity reaching a maximum 24 h post-surgery. Significant loss of p75 low-affinity neurotrophin receptor immunoreactivity and of cortical AChE-positive projections only became apparent 48 h post-lesion. Carbocyanine 3-192IgG labelling in the ipsilateral basal forebrain exceeded that of the contralateral hemisphere at all time points investigated and progressively declined in the damaged magnocellular nucleus basalis up to 48 h after NMDA infusion. The present study indicates that excitotoxic lesion-induced alteration of cholinergic neuronal markers is a rapid and gradual process reaching its maximum 24 h post-surgery. Furthermore, in vivo labelling of cholinergic neurons may be applied to indicate neuronal survival under pathological conditions, and enable to follow their degeneration process under a variety of experimental conditions.


Subject(s)
Basal Nucleus of Meynert/drug effects , Basal Nucleus of Meynert/pathology , Excitatory Amino Acid Agonists/toxicity , N-Methylaspartate/toxicity , Neurons/chemistry , Acetylcholine/physiology , Animals , Carbocyanines/pharmacokinetics , Choline O-Acetyltransferase/analysis , Choline O-Acetyltransferase/metabolism , Fluoresceins , Fluorescent Dyes/pharmacokinetics , Immunoglobulin G , Immunohistochemistry/methods , Injections, Intraventricular , Male , Microscopy, Confocal , Nerve Degeneration/chemically induced , Nerve Degeneration/pathology , Neural Pathways , Neuroglia/metabolism , Neurons/enzymology , Neurons/pathology , Neurotoxins/toxicity , Organic Chemicals , Rats , Rats, Wistar , Receptor, Nerve Growth Factor/analysis , Receptor, Nerve Growth Factor/metabolism , Receptors, N-Methyl-D-Aspartate/analysis , Sensitivity and Specificity
5.
Neuroscience ; 108(4): 629-42, 2001.
Article in English | MEDLINE | ID: mdl-11738499

ABSTRACT

Recent evidence indicates that stimulation of postsynaptic 5-HT(1A) receptors abates excitotoxic neuronal death. Here we investigated whether oral post-lesion administration of the 5-HT(1A) receptor agonist (-)-(R)-2-[4-[[(3,4-dihydro-2H-1-benzopyran-2-yl)methyl]amino]butyl]-1,2-benzisothiazol-3(2H)-one 1,1-dioxide monohydrochloride (Repinotan HCl) attenuates N-methyl-D-aspartate (NMDA) excitotoxicity (60 nmol/microl) in the rat magnocellular nucleus basalis. Repinotan HCl (1 mg/kg) was administered from day 1, 2, 3, or 6 post-surgery twice daily for five consecutive days. This delayed drug administration protocol was employed to investigate the initiation period during which 5-HT(1A) receptor agonists may significantly influence ongoing neurodegeneration processes. 8-Hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT, 1 mg/kg) served as reference compound. Twenty-four hours after drug delivery a small open-field test, while on day 14 post-surgery a passive avoidance test was performed. Effects of Repinotan HCl treatment on the survival of cholinergic magnocellular nucleus basalis neurons and their cortical projections were determined by quantitative acetylcholinesterase (AChE) and choline-acetyltransferase (ChAT) histochemistry. Moreover, AChE and ChAT activities were biochemically measured both in the cerebral cortex and in the magnocellular nucleus basalis. Repinotan HCl treatment markedly increased spontaneous activities in the small open-field at any time-point investigated. Improved memory performance was only demonstrated when Repinotan HCl was administered from day 1 post-lesion on wards. Repinotan HCl treatment from day 2 and 3 post-lesion on markedly attenuated both histochemical and neurochemical characteristics of NMDA excitotoxicity on cholinergic magnocellular nucleus basalis neurons and on their cortical projections. Whereas the neuroprotective profile of Repinotan HCl was superior to that of 8-OH-DPAT, oral administration of both 5-HT(1A) receptor agonists yielded largely equivalent behavioral recovery after NMDA infusion in the magnocellular nucleus basalis. In conclusion, the present data indicate the potent neuroprotective action of the 5-HT(1A) receptor agonist Repinotan HCl with a peak efficacy of delayed (2-3 day) post-lesion drug treatment in vivo. Post-lesion treatment with 5-HT(1A) receptor agonists may therefore be of significance in the intervention of neuronal damage associated with acute excitotoxic conditions.


Subject(s)
Apoptosis/drug effects , Basal Nucleus of Meynert/pathology , Benzopyrans/pharmacology , Receptors, Serotonin/metabolism , Serotonin Receptor Agonists/pharmacology , Thiazoles/pharmacology , 8-Hydroxy-2-(di-n-propylamino)tetralin/pharmacology , Acetylcholine/physiology , Administration, Oral , Animals , Basal Nucleus of Meynert/drug effects , Behavior, Animal/drug effects , Choline O-Acetyltransferase/analysis , Excitatory Amino Acid Agonists/toxicity , Male , Memory/drug effects , N-Methylaspartate/toxicity , Neurons/enzymology , Neurons/pathology , Neuroprotective Agents/pharmacology , Parietal Lobe/pathology , Rats , Rats, Wistar , Receptors, Serotonin, 5-HT1
6.
J Neuroendocrinol ; 13(9): 749-60, 2001 Sep.
Article in English | MEDLINE | ID: mdl-11578524

ABSTRACT

Extensive studies during the past decades provided compelling evidence that glucocorticoids (GCs) have the potential to affect the development, survival and death of neurones. These observations, however, reflect paradoxical features of GCs, as they may be critically involved in both neurodegenerative and neuroprotective processes. Hence, we first address different aspects of the complex role of GCs in neurodegeneration and neuroprotection, such as concentration dependent actions of GCs on neuronal viability, anatomical diversity of GC-mediated mechanisms in the brain and species and strain differences in GC-induced neurodegeneration. Second, the modulatory action of GCs during development and ageing of the central nervous system, as well as the contribution of altered GC balance to the pathogenesis of neurodegenerative disorders is considered. In addition, we survey recent data as to the possible mechanisms underlying the neurodegenerative and neuroprotective actions of GCs. As such, two major aspects will be discerned: (i) GC-dependent offensive events, such as GC-induced inhibition of glucose uptake, increased extracellular glutamate concentration and concomitant elevation of intracellular Ca(2+), decrease in GABAergic signalling and regulation of local GC concentrations by 11 beta-hydroxysteroid dehydrogenases; and (ii) GC-related cellular defence mechanisms, such as decrease in after-hyperpolarization, increased synthesis and release of neurotrophic factors and lipocortin-1, feedback regulation of Ca(2+) currents and induction of antioxidant enzymes. The particular relevance of these mechanisms to the neurodegenerative and neuroprotective effects of GCs in the brain is discussed.


Subject(s)
Glucocorticoids/physiology , Neurons/physiology , Cell Survival/physiology , Glucocorticoids/pharmacology , Nerve Degeneration/physiopathology , Neuroprotective Agents/pharmacology
7.
Behav Neurosci ; 115(5): 1111-7, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11584924

ABSTRACT

This study examined the consequences of elevated corticosterone levels in lactating rats on their offspring's serotonergic 5-hydroxytryptamine (5-HT)1A receptor system and behavioral coping with stress. The mothers received normal drinking water or water with corticosterone, which, via the milk, enters the circulation and brains of the pups. In adulthood, the corticosterone-nursed offspring showed a consistently more passive way of coping with environmental challenges. However, they did not seem to be more anxious. Autoradiographic analysis of the 5-HT1A receptor system revealed a decrease in the adult 5-HT1A receptor binding in the hippocampal CA1 region. The results support the hypothesis that differences in behavioral coping with stress by adult rats are associated with differences in the serotonergic system. At the same time, it suggests that adult coping and its neuronal substrates are not solely determined by genes but depend on subtle developmental factors as well.


Subject(s)
Adaptation, Psychological/physiology , Arousal/physiology , Corticosterone/blood , Hippocampus/physiology , Prenatal Exposure Delayed Effects , Receptors, Serotonin/physiology , Aggression/physiology , Animals , Autoradiography , Brain Mapping , Exploratory Behavior/physiology , Fear/physiology , Female , Lactation/physiology , Maze Learning/physiology , Pregnancy , Rats , Rats, Wistar , Receptors, Serotonin, 5-HT1 , Social Environment
8.
Physiol Behav ; 72(3): 349-54, 2001 Feb.
Article in English | MEDLINE | ID: mdl-11274676

ABSTRACT

The experiments explored the nature and time course of changes in glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) binding in homogenates of various brain regions and pituitary of male Wistar rats following social defeat stress. One week after defeat, the binding capacity of GRs was decreased in the hippocampus and the hypothalamus while no changes were observed in the parietal cortex and the pituitary. The number of MRs remained at the same level as in undefeated rats. Three weeks postdefeat, the initially down-regulated GR returned to baseline level in the hippocampus and the hypothalamus. However, GR binding was now decreased in the parietal cortex. Severe down-regulation of MRs was detected in the hippocampal and septal tissue. The results show that brief but intense stress like social defeat induces a long-lasting down-regulation of corticosteroid receptors and that the temporal dynamics of these changes are not only differential for GRs and MRs but also for brain sites.


Subject(s)
Aggression/physiology , Brain Chemistry/physiology , Down-Regulation/physiology , Receptors, Steroid/biosynthesis , Social Dominance , Animals , Blotting, Western , Brain/anatomy & histology , Male , Organ Size/physiology , Rats , Rats, Wistar , Thymus Gland/physiology , Time Factors
9.
Endocrine ; 15(3): 287-90, 2001 Aug.
Article in English | MEDLINE | ID: mdl-11762701

ABSTRACT

The exact contribution of corticosteroids to the control of prolactin secretion in lactating rats is poorly understood. Therefore, the present studies were focused on the effect of adrenalectomy and dexamethasone treatment on the suckling-induced prolactin release. Animals were adrenalectomized on the 3rd day of lactation and tested on the 7th day of lactation. In adrenalectomized animals, the suckling stimulus failed to induce the characteristic increase in plasma prolactin levels. Dexamethasone pretreatment (400 microg/kg b.w. s.c. 24, 48, 72 h before testing) of adrenalectomized rats restored this prolactin response. The same treatment with dexamethasone given to control animals attenuated the suckling stimulus induced prolactin response. The present findings indicate that corticosteroids are essential for a basic prolactin response of lactating rats.


Subject(s)
Animals, Suckling/physiology , Feeding Behavior/physiology , Glucocorticoids/physiology , Prolactin/metabolism , Adrenalectomy , Animals , Dexamethasone/pharmacology , Radioimmunoassay , Rats , Rats, Sprague-Dawley
10.
Brain Res Bull ; 56(6): 589-92, 2001 Dec.
Article in English | MEDLINE | ID: mdl-11786246

ABSTRACT

The contribution of corticosteroids to the control of prolactin secretion in lactating rats was investigated. The prolactin response to domperidone (20 microg/kg b.w., i.v.), a dopamine receptor antagonist and to domperidone plus formalin stress was tested in adrenalectomized and/or dexamethasone-treated continuously nursing rats. Animals were adrenalectomized on the 3rd day of lactation and tested on the 7th day of lactation. Dexamethasone was injected s.c. 24 h before testing (400 microg/kg b.w.) and on the day of testing (200 microg/kg b.w.). Domperidone caused a significant rise in plasma prolactin levels. The prolactin response to domperidone was twice as high in solely adrenalectomized dams and in solely dexamethasone-treated rats compared to controls. In adrenalectomized animals treated with dexamethasone, the prolactin response to domperidone was like in controls. Formalin injection to either adrenalectomized plus domperidone-treated animals or to animals injected with dexamethasone plus domperidone, resulted in a statistically significant depletion of plasma prolactin. In controls and in adrenalectomized animals receiving dexamethasone and domperidone, the prolactin response to formalin was very similar, i.e., plasma prolactin levels did not change after the administration of formalin. The present findings suggest that in lactating rats, corticosteroids are involved in the prolactin response to domperidone and to formalin stress.


Subject(s)
Dopamine/metabolism , Hypothalamo-Hypophyseal System/metabolism , Lactation/physiology , Pituitary-Adrenal System/metabolism , Prolactin/metabolism , Receptors, Dopamine/metabolism , Stress, Physiological/metabolism , Adrenalectomy/adverse effects , Animals , Dexamethasone/pharmacology , Domperidone/pharmacology , Dopamine Antagonists/pharmacology , Female , Formaldehyde/pharmacology , Glucocorticoids/pharmacology , Hypothalamo-Hypophyseal System/drug effects , Lactation/drug effects , Pain Measurement , Pituitary-Adrenal System/drug effects , Prolactin/blood , Rats , Rats, Sprague-Dawley , Receptors, Dopamine/drug effects , Stress, Physiological/physiopathology
11.
Neuroscience ; 101(1): 101-14, 2000.
Article in English | MEDLINE | ID: mdl-11068140

ABSTRACT

In the present study plastic neural responses to N-methyl-D-aspartate-induced excitotoxic lesions and the neuroprotective effects of the L-type voltage-dependent Ca(2+) channel antagonist nimodipine were investigated in the rat magnocellular nucleus basalis. Assessment of spontaneous behaviour in the elevated plus maze and small open-field paradigms on day 5 and day 14 post-surgery indicated anxiety and persistent hypoactivity of N-methyl-D-aspartate-lesioned rats, as compared with sham-operated controls. Nimodipine administration significantly alleviated the behavioural deficits. Quantitative histochemical analysis of acetylcholinesterase-positive fibre innervation of the somatosensory cortex and determination of the numbers of choline-acetyltransferase-positive proximal fibre branches of cholinergic projection neurons in the magnocellular nucleus basalis demonstrated a severe cholinergic deficit as a consequence of the excitotoxic lesion 14 days post-surgery. Nimodipine pre-treatment significantly attenuated the loss of cortical cholinergic innervation and preserved the functional integrity of cholinergic projection neurons in the magnocellular nucleus basalis. Double-labelling immunocytochemistry demonstrated increased amyloid precursor protein expression in shrinking and presumably apoptotic choline-acetyltransferase-positive neurons, whereas surviving cholinergic nerve cells were devoid of excessive amyloid precursor protein immunoreactivity. Moreover, as a consequence of N-methyl-D-aspartate infusion, rim-like accumulation of amyloid precursor protein-positive astrocytes was visualized in a penumbra-like zone of the excitotoxic injury. Furthermore, abundant sprouting of serotonergic projection fibres invading the damaged magnocellular nucleus basalis subdivision was demonstrated. Pharmacological blockade by the Ca(2+) antagonist nimodipine significantly attenuated both neuronal and glial amyloid precursor protein immunoreactivity and serotonergic fibre sprouting following N-methyl-D-aspartate infusion. The present data characterize plastic endogenous glial and neuronal responses in the magnocellular nucleus basalis model of acute excitotoxic brain damage. The increased amyloid precursor protein expression may indicate effective means of intrinsic neuroprotection, as secreted amyloid precursor protein isoforms are suggested to play a role in neuronal rescue following excitotoxic injury. From a pharmacological point of view, extensive sprouting of serotonergic projections in the damaged magnocellular nucleus basalis may also counteract N-methyl-D-aspartate excitotoxicity via serotonin-induced inhibition of Ca(2+) currents and membrane hyperpolarization. Hence, lesion-induced changes in spontaneous animal behaviour, such as anxiety and novelty-induced hypoactivity, may well be attributed to the considerable re-distribution of serotonergic projections in the basal forebrain. In conclusion, our present data emphasize a role of neuron-glia and neurotransmitter-system interactions in functional recovery after acute excitotoxic brain injury, and the efficacy of L-type Ca(2+) channel blockade by the selective 1,4-dihydropyridine antagonist nimodipine.


Subject(s)
Amyloid beta-Protein Precursor/metabolism , Axons/drug effects , Basal Nucleus of Meynert/drug effects , Neuronal Plasticity/drug effects , Neuroprotective Agents/pharmacology , Nimodipine/pharmacology , Serotonin/metabolism , Animals , Axons/metabolism , Axons/ultrastructure , Basal Nucleus of Meynert/metabolism , Calcium Channels, L-Type/drug effects , Calcium Channels, L-Type/metabolism , Choline O-Acetyltransferase/metabolism , Denervation/adverse effects , Male , Maze Learning/drug effects , Maze Learning/physiology , N-Methylaspartate/adverse effects , Nerve Degeneration/chemically induced , Nerve Degeneration/drug therapy , Nerve Degeneration/metabolism , Nerve Regeneration/drug effects , Nerve Regeneration/physiology , Neural Pathways/drug effects , Neural Pathways/metabolism , Neuronal Plasticity/physiology , Neurotoxins/adverse effects , Rats , Rats, Wistar
12.
Eur J Pharmacol ; 405(1-3): 33-42, 2000 Sep 29.
Article in English | MEDLINE | ID: mdl-11033312

ABSTRACT

It has been reported that the ACTH-(4-9) analog H-Met(O(2))-Glu-His-Phe-D-Lys-Phe-OH (ORG 2766) administered in adulthood has trophic effects on neuronal tissue and when given postnatally, it can induce long-lasting changes in brain development. In the present study, we investigated whether early postnatal treatment with ORG 2766 affects adult neuronal vulnerability, i.e. the sensitivity of cholinergic neurons against excitotoxic damage. Wistar rat pups received injections of ORG 2766 or saline on postnatal days 1, 3 and 5 and were then left undisturbed until adulthood. At the age of 6 months, the animals were subjected to unilateral lesion of magnocellular basal nucleus by infusion of high dose of N-methyl-D-aspartate (NMDA). The effects of the excitotoxic insult were studied 28 hours and 12 days after the lesion by measuring both the acute cholinergic and glial responses, and the final outcome of the degeneration process. Twenty eight hours after NMDA infusion, postnatally ACTH-(4-9)-treated animals showed stronger suppression of choline-acetyltransferase immunoreactivity and increased reaction of glial fibrillary acidic protein -immunopositive astrocytes in the lesioned nucleus compared to control animals. However, 12 days post-surgery, the NMDA-induced loss of cholinergic neurons, as well as the decrease of their acetylcholinesterase -positive fibre projections in the cortex, were less in ACTH-(4-9) animals. Our data indicate that the early developmental effects of ACTH-(4-9) influence intrinsic neuroprotective mechanisms and reactivity of neuronal and glial cells, thereby resulting in a facilitated rescuing mechanism following excitotoxic injury.


Subject(s)
Adrenocorticotropic Hormone/analogs & derivatives , Adrenocorticotropic Hormone/pharmacology , Animals, Newborn/physiology , Basal Nucleus of Meynert/drug effects , Excitatory Amino Acid Antagonists/pharmacology , N-Methylaspartate/antagonists & inhibitors , Peptide Fragments/pharmacology , Acetylcholinesterase/metabolism , Animals , Astrocytes/drug effects , Basal Nucleus of Meynert/enzymology , Basal Nucleus of Meynert/pathology , Cell Count , Choline O-Acetyltransferase/metabolism , Excitatory Amino Acid Agonists/toxicity , Glial Fibrillary Acidic Protein/metabolism , Immunohistochemistry , N-Methylaspartate/toxicity , Rats , Rats, Wistar
13.
Brain Res ; 873(1): 140-4, 2000 Aug 04.
Article in English | MEDLINE | ID: mdl-10915821

ABSTRACT

Investigating the cellular events in the pituitary gland, the intracellular cyclic AMP (cAMP) of the neural lobe (NL), intermediate lobe (IL), the inner (IZ-AL) and outer zone (OZ-AL) of the anterior lobe (AL) have been measured during the suckling stimulus. Ten-minutes suckling, parallel to the elevation of plasma PRL, induced a significant increase of cAMP concentration in the IZ-AL. In contrast, 10- and 30-min suckling resulted in a decrease of cAMP level in the NL. Changes in cAMP of the OZ-AL and the IL as well as in the plasma level of alpha-MSH could not be detected. These region-specific changes of cAMP in the pituitary gland during suckling stimulus seems to be related to interacting neuroendocrine signals delivered concomitantly from the hypothalamus and from the NIL to the IZ-AL.


Subject(s)
Cyclic AMP/metabolism , Lactation/physiology , Pituitary Gland, Anterior/metabolism , Animals , Female , Pituitary Gland, Posterior/metabolism , Prolactin/blood , Rats , Time Factors , Tissue Distribution
14.
J Neuroendocrinol ; 12(6): 486-94, 2000 Jun.
Article in English | MEDLINE | ID: mdl-10844576

ABSTRACT

The impact of glucocorticoids on beta-amyloid(1-42) (Abeta(1-42)) and NMDA-induced neurodegeneration was investigated in vivo. Abeta(1-42) or NMDA was injected into the cholinergic magnocellular nucleus basalis in adrenalectomized (ADX) rats, ADX rats supplemented with 25%, 100%, 2x100% corticosterone pellets, or sham-ADX controls. Abeta(1-42)- or NMDA-induced damage of cholinergic nucleus basalis neurones was assessed by quantitative acetylcholinesterase histochemistry. Plasma concentrations of corticosterone and cholinergic fibre loss after Abeta(1-42) or NMDA injection showed a clear U-shaped dose-response relationship. ADX and subsequent loss of serum corticosterone potentiated both the Abeta(1-42) and NMDA-induced neurodegeneration. ADX+25% corticosterone resulted in a 10-90 nM plasma corticosterone concentration, which significantly attenuated the Abeta(1-42) and NMDA neurotoxicity. ADX+100% corticosterone (corticosterone concentrations of 110-270 nM) potently decreased both Abeta(1-42)- and NMDA-induced neurotoxic brain damage. In contrast, high corticosterone concentrations of 310-650 nM potentiated Abeta(1-42)- and NMDA-triggered neurodegeneration. In conclusion, chronic low or high corticosterone concentrations increase the vulnerability of cholinergic cells to neurotoxic insult, while slightly elevated corticosterone levels protect against neurotoxic injury. Enhanced neurotoxicity of NMDA in the presence of high concentrations of specific glucocorticoid receptor agonists suggests that the corticosterone effects are mediated by glucocorticoid receptors.


Subject(s)
Basal Nucleus of Meynert/drug effects , Basal Nucleus of Meynert/pathology , Corticosterone/administration & dosage , Nerve Degeneration/chemically induced , Nerve Degeneration/pathology , Neuroprotective Agents/administration & dosage , Amyloid beta-Peptides/pharmacology , Animals , Corticosterone/pharmacology , Dose-Response Relationship, Drug , Excitatory Amino Acid Agonists/pharmacology , Male , N-Methylaspartate/pharmacology , Neuroprotective Agents/pharmacology , Peptide Fragments/pharmacology , Rats , Rats, Wistar , Time Factors
15.
J Neuroendocrinol ; 11(12): 925-33, 1999 Dec.
Article in English | MEDLINE | ID: mdl-10583727

ABSTRACT

Environmental stimuli during early stages of life can influence the development of an organism and may result in permanent changes in adult behaviour and physiology. In the present study we investigated the influence of early postnatal handling on adult neuroendocrine and behavioural stress reactivity in Wistar rats. Pups were subjected to handling from postnatal day 1-21. The young were taken from the nest every day for 15 min and each of the pups was handled separately. Control nests were left undisturbed. When the animals had reached an adult age of 3-4 months they were individually housed and subjected to a series of tests to measure their stress reactivity. In the first experiment we established adult behavioural coping with stressors and anxiety in the following series of tests: open field test, shock prod defensive burying test, elevated plus maze and conditioned fear test. Collectively, the data clearly indicate that handled animals are characterized by a lower stress-induced anxiety. Yet, handled and control animals do not differ in their general way of coping with stressors. Although the lower anxiety in handled animals is often reflected in a higher activity, they are not more active per se. In a second experiment, animals were provided with a permanent jugular vein canula for repeated blood sampling to determine stress hormones: noradrenaline, adrenaline, prolactin and corticosterone. Animals were subjected to a novelty test and a conditioned fear test. The neuroendocrine response profile is consistent with the conclusion that handled animals are less anxious than controls but are not different in their general strategy of coping with stressors. The handled animals showed an attenuated adrenaline, prolactin and corticosterone response. Yet, in neither of the two tests there was a difference in noradrenaline response, a typical marker for an active coping strategy. Interestingly, the differences in neuroendocrine reactivity already appeared in response to a mild novelty challenge when there were no clear behavioural differences yet. The neuroendocrine measures are in line with the behavioural data but more sensitively reflect the differences between handled and control animals.


Subject(s)
Behavior, Animal/physiology , Neurosecretory Systems/physiology , Stress, Physiological/physiopathology , Adaptation, Psychological/physiology , Age Factors , Animals , Anxiety/physiopathology , Conditioning, Psychological/physiology , Corticosterone/blood , Electroshock , Epinephrine/blood , Fear , Female , Male , Maze Learning/physiology , Norepinephrine/blood , Prolactin/blood , Rats , Rats, Wistar , Restraint, Physical
16.
Behav Brain Res ; 106(1-2): 181-8, 1999 Dec.
Article in English | MEDLINE | ID: mdl-10595434

ABSTRACT

Studies on adult animals and humans have shown that the ACTH4-9 analog ORG 2766 influences cognitive performance and possibly has neurotrophic effects. For this reason we studied the effect of ORG 2766 applied in early postnatal life when brain structures and neuronal pathways are still developing. Our aim was to see whether such treatment during development would result in permanent changes in adult behavioural performance. Pups received subcutaneous injections of 1 microg/g bodyweight ACTH4-9 analog ORG 2766 on day 1, 3 and 5 after birth. Control animals in the same nest received saline injections. When the animals had reached an adult age of 3 months they were subjected to a series of tests to measure their behavioural performance. In the first experiment, behavioural stress responses and anxiety were measured by subjecting the rats to the following tests: open field, defensive burying, elevated plus maze, and conditioned fear test. In a second experiment, adult cognitive function was measured in the Morris water-maze, a hippocampus-related spatial learning test, and in the active avoidance test, a more amygdala-related nonspatial test. The results showed that animals treated with ORG 2766 during early postnatal life learned faster in the spatial Morris water-maze. The treatment had a positive effect on performance during the acquisition phase of the learning task, while memory retrieval was not affected. Learning in the nonspatial active avoidance task did not change due to the postnatal ACTH4-9 treatment. In addition, there were no differences in the open field test, the defensive burying test, elevated plus maze and the conditioned fear test. The latter supports the conclusion that the differences in water-maze performance was due to a difference in learning speed, rather than a difference in anxiety or behavioural stress reactivity. Analysis of [3H]CORT binding capacity measured after the learning tests revealed no differences in the hippocampal MR and GR concentration between non-treated and treated animals.


Subject(s)
Adrenocorticotropic Hormone/analogs & derivatives , Animals, Newborn/physiology , Peptide Fragments/pharmacology , Space Perception/drug effects , Stress, Psychological/psychology , Adrenocorticotropic Hormone/administration & dosage , Adrenocorticotropic Hormone/pharmacology , Animals , Anxiety/psychology , Electroshock , Learning/drug effects , Learning/physiology , Maze Learning/drug effects , Memory/drug effects , Memory/physiology , Motor Activity/drug effects , Motor Activity/physiology , Peptide Fragments/administration & dosage , Rats , Rats, Wistar , Receptors, Steroid/drug effects
17.
Mol Cell Endocrinol ; 149(1-2): 1-7, 1999 Mar 25.
Article in English | MEDLINE | ID: mdl-10375012

ABSTRACT

In lactating rats, suckling renders mammotropes more responsive to prolactin (PRL)-releasing stimuli and less responsive to PRL-inhibiting secretagogues. We have previously shown that a decrease in the activity of protein phosphatase 2A (PP2A) may be responsible for the decrease in responsiveness to the inhibitory secretagogue dopamine (DA). In our present experiments, we have studied the involvement of the adenylate cyclase (AC), stimulatory and inhibitory GTP-binding proteins and also the role of PP2A in the sensitization phenomenon. Pituitary cells obtained from mother rats separated from their pups for 4 h prior to dispersion (non-suckled), suckled for 10 or 30 min after the separation period (suckled) and without separation (continual suckling) were incubated in the presence of different doses of forskolin to activate AC and DA. In a further study, pituitary cells of non-suckled rats were pretreated with cholera toxin (CTX) or pertussis toxin (PTX) and tested for the stimulatory action of forskolin or TRH on PRL release. Ocadaic acid (OA) pretreatment has been used to investigate the involvement of PP2A. Hormone secretion was measured by the reverse hemolytic plaque assay (RHPA). Our results have shown that cells from non-suckled rats were unresponsive to forskolin. A 10-min suckling stimulus sensitizes pituitary mammotropes to respond with a PRL release to a dose-dependent activation of AC by forskolin. This sensitization of AC becomes a permanent feature of the cells when suckling continues for an additional 20 min. We have also found that pituitary mammotropes from non-suckled dams respond to forskolin or TRH with PRL release when they were preincubated with either PTX or the PP2A inhibitor OA. It clearly indicates that the non-responsive pituitary can be shifted to the responsive stage by uncoupling of inhibitory G-protein from its receptor as well as by inhibition of PP2A. This latter finding, consonant with our previous results, suggests that suckling may cause selective changes in the function of G(i) of mammotropes due to a rapid phosphorylation which can remove tonic, GTP-dependent inhibitory function.


Subject(s)
Adenylyl Cyclases/physiology , GTP-Binding Proteins/physiology , Lactation/physiology , Phosphoprotein Phosphatases/antagonists & inhibitors , Pituitary Gland, Anterior/physiology , Adenylate Cyclase Toxin , Animals , Cholera Toxin/pharmacology , Colforsin/pharmacology , Dopamine/pharmacology , Female , Okadaic Acid/pharmacology , Pertussis Toxin , Pituitary Gland, Anterior/drug effects , Pregnancy , Prolactin/metabolism , Protein Phosphatase 2 , Rats , Rats, Sprague-Dawley , Sucking Behavior/physiology , Thyrotropin-Releasing Hormone/pharmacology , Virulence Factors, Bordetella/pharmacology
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