Formulation of thermoresponsive and bioadhesive gel for treatment of oesophageal pain and inflammation.

The aim of this study was the formulation and examination of a novel thermoresponsive and bioadhesive, in situ gelling drug delivery system, which can be used in the treatment of oesophageal pain and inflammation. A bioadhesive cellulose derivative (Metolose) 60SH) was used as a thermoresponsive material, because Metolose has thermal gelation properties at certain temperature. The thermal gelation temperature (T(2)) of Metolose 60SH 2 w/w% solution is above body temperature (65-66 degrees C), but by using different methods (Metolose 60SH concentration, auxiliary materials), it can be shifted near to body temperature. The pH alteration between pH=2-10 and the application of different alcohols did not influence the gelation temperature, but using water-soluble salts and changing the concentration of Metolose 60SH solution between 2 and 3 w/w% the thermal gelation point could be decreased. Different NSAIDs were used as model drugs and which had not influence on thermal gelation temperature, but difference in in vitro liberation and penetration can be observed. In vitro adhesion test pointed out that the condition of investigated membrane can change the adhesion. Morphological test of oesophageal tissue showed that investigated materials had no irritative or tissue-damaging effect on the oesophageal mucosa even after 12h.

Which molecularly imprinted polymer is better?

Molecularly imprinted polymers (MIP) have been successfully synthesized toward many different compounds in the last decades. The mechanistic details of selective binding at binding sites are not yet well understood. For this reason the characterization of MIP binding has been mostly phenomenological and this makes the transfer of results between different laboratories or between different types of applications difficult. In this paper we analyze the relationship between different types of characterization like isotherms, binding site models, chromatographic k and alpha values, etc. as they relate to different applications like HPLC, solid phase extraction (SPE), binding assays, batch extraction and sensors. It is shown that alpha values determined by elution chromatography depend on seemingly irrelevant factors as the length and diameter of the column, respectively. The determination of distribution ratios or partition coefficients is proposed as an easily understandable and useful quantity in the characterization of novel MIPs. Data used for the characterization of a MIP should be transferable between different applications but the qualification of MIPs as better or worse will depend on the application in case.

Nonlinear adsorption isotherm as a tool for understanding and characterizing molecularly imprinted polymers.

Molecularly imprinted polymers (MIPs) have frequently been characterized by quantities which are easily determined from experiments but have no theoretical foundation. This makes it difficult to compare different MIP preparations or to transfer MIP based methods to different experimental conditions. Since the adsorption isotherms of MIPs are markedly nonlinear, one can build a better characterization strategy on isotherms as shown by examples in this paper.

Chromatographic behavior of silica-polymer composite molecularly imprinted materials.

Molecularly imprinted polymers (MIP) have recently been prepared inside the pores of silica based HPLC packing materials. Detailed physical and chromatographic characterization of such a silica-MIP composite material is presented. The chromatographic peak shape obtained with the uniformly sized spherical silica-MIP composite is mainly determined by the nonlinear adsorption isotherm. Comparison of the composite with the conventional sieved and grinded bulk MIP is therefore based on the nonlinear isotherm and not on retention factors and plate numbers.

The publication speed of information in bibliographic chemical databases.

The quality of bibliographic databases depends very much on the reliably fast follow-up of the pertinent literature. We have studied this quality feature of two important chemical bibliographic databases: Chemical Abstracts (CA) and Analytical Abstracts (AA). The follow-up rate (speed of information) of these databases has been determined with respect to 10 core journals in analytical chemistry. On the average the performance of Chemical Abstracts has been good over the past decade, while the formerly poor performance of Analytical Abstracts has improved considerably. Some quite unexpected nonuniformities can, however, still be detected in the publication time distributions of both databases.

Publication speed in analytical chemistry journals.

Variations of the time elapsing between submission or acceptance and publication ("lapse time") of papers have been studied for 10 major analytical chemistry journals, for the 1985-1999 period. The time from receipt of all manuscripts up to their publication and the period between their acceptance and their publication were analyzed in four selected years: 1985, 1990, 1995, and 1999. The majority of the investigated journals showed good average performance with sometimes disturbing fluctuations. Talanta has not been able to catch up with the rest, while the Analyst has recently become clear leader in publication speed.

Determination of deramciclane and N-desmethylderamciclane in human plasma by liquid chromatography-tandem mass spectrometry using off-line robotic sample pretreatment.

A rapid and highly sensitive LC-MS-MS method using deuterium-labelled internal standards was developed and evaluated for the simultaneous determination of deramciclane and its pharmacologically active metabolite (N-desmethylderamciclane). The sample preparation based on liquid-liquid extraction was carried out with an off-line robotic system. Evaluation of this analytical method shows that samples can be assayed with acceptable accuracy and precision in the 0.1 to 50 ng/ml concentration range for both compounds. The method was applied for the quantitative determination of deramciclane and its metabolite in human plasma samples during a food interaction pharmacokinetic study.

A revisited auditing of the analytical abstracts database

This paper is a follow-up of a previous one dealing with the "Image of Analytical Chemistry as Reflected in the Analytical Abstracts Database: Journal Coverage, Concentration and Dispersion of the Analytical Literature" (J. Chem. Inf. Comput. Sci. 1993, 33, 164-173). It deals with revisiting these topics. The results have shown that the database has substantially improved its coverage by editorial reorganizations in 1994. The only open problem which has been revealed is a somewhat excessive emphasis given to the coverage of the journals on the lower tail of the journal distribution. The suggestion is made to reduce this emphasis in favor of an even more complete coverage of some "titled" analytical journals.

Selective trace enrichment of chlorotriazine pesticides from natural waters and sediment samples using terbuthylazine molecularly imprinted polymers

Two molecularly imprinted polymers were synthesized using either dichloromethane or toluene as the porogen and terbuthylazine as the template and were used as solid-phase extraction cartridges for the enrichment of six chlorotriazines (deisopropylatrazine, deethylatrazine, simazine, atrazine, propazine, and terbuthylazine) in natural water and sediment samples. The extracted samples were analyzed by liquid chromatography/diode array detection (LC/DAD). Several washing solvents, as well as different volumes, were tested for their ability to remove the matrix components nonspecifically adsorbed on the sorbents. This cleanup step was shown to be of prime importance to the successful extraction of the pesticides from the aqueous samples. The optimal analytical conditions were obtained when the MIP imprinted using dichloromethane was the sorbent, 2 mL of dichloromethane was used in the washing step, and the preconcentrated analytes were eluted with 8 mL of methanol. The recoveries were higher than 80% for all the chlorotriazines except for propazine (53%) when 50- or 100-mL groundwater samples, spiked at 1 microg/L level, were analyzed. The limits of detection varied from 0.05 to 0.2 microg/L when preconcentrating a 100-mL groundwater sample. Natural sediment samples from the Ebre Delta area (Tarragona, Spain) containing atrazine and deethylatrazine were Soxhlet extracted and analyzed by the methodology developed in this work. No significant interferences from the sample matrix were noticed, thus indicating good selectivity of the MIP sorbents used.

Immunoassay-based determination of phenobarbital using size-exclusion chromatography.

The measurement of the anti-epileptic drug phenobarbital from serum samples combining immunoassay and size-exclusion chromatography is presented. The immunoreaction is based on the competitive binding of the analyte (unlabelled phenobarbital) and the fluorescent-labelled phenobarbital to anti-phenobarbital antibodies. Mixing of the reagents and the immunoreaction takes place in a flow system. The products are separated on-line on a short gel chromatographic column and the fluorescence intensity of the marker is measured. The calibration curve shows good linearity in the range 5-80 microg/ml, corresponding to therapeutically relevant serum levels. Intra-day precision values are between 7.32 and 9.48%; the accuracy is between 0.97 and 9.43%. Inter-day precision and accuracy measured on 6 different days fall between 5.38 and 10.05% and -8.27 and -4.97%, respectively. The results obtained with the proposed method show a good correlation with those of other methods (radioimmunoassay and fluorescence polarisation immunoassay) already established in clinical laboratories.

Microstructure of Ion-Selective Plasticized PVC Membranes Studied by Small-Angle Neutron Scattering.

The microstructure of plasticized PVC membranes in the dry state and during the process of soaking in heavy water has been studied by small-angle neutron scattering. In the dry membrane, inhomogeneities were found. The membrane structure is well described by a polydisperse hard-sphere model. The mean diameter of the dispersed spherical inhomogeneities is ∼6 nm, which is smaller than the estimated dimension of a single statistically curled PVC polymer chain in the membrane. The values of the best-fit parameters and their change with membrane composition suggest that the particles consist of unplasticized PVC, probably in the crystalline state. The type of plasticizer, the plasticizer content, and the addition of a lipophilic salt were found to influence the water uptake significantly. Water uptake did not change the microstructure due to the original (i.e., dry state) inhomogeneities in the membranes.

Food interaction pharmacokinetic study of cordaflex 20 mg retard filmtablet in healthy volunteers.

The aim of the present study was to investigate the effect of food consumption on the pharmacokinetics of Cordaflex 20 mg retard filmtablet in healthy volunteers through measuring nifedipine plasma levels by an HPLC-ED method both after fasting and food ingestion. The food interaction pharmacokinetic study of Cordaflex 20 mg retard filmtablet was carried out in 12 healthy male volunteers treated with a single dose of the preparation both after fasting and after food ingestion, in a crossover design allowing 1 week of wash-out period between the 2 treatments. Nifedipine concentration of plasma samples were determined by an isocratic HPLC-ED method [Horvai et al. 1994] with robotic sample processing [Horváth et al. 1995, 1996]. The pharmacokinetic parameters (AUC0-infinity, AUC0-t, Cmax, MRT) were analyzed by calculating 90% confidence interval for logarithmic transformed test/reference ratio values, and Schuirmann's statistical tests, the tmax and HVD values were analyzed by Wilcoxon's nonparametric statistical test. The above statistical tests of the present food interaction study indicated significant differences for each one of the respective pharmacokinetic parameter pairs calculated for treatments after fasting and after food ingestion. On the basis of the above findings and also by comparing the mean pharmacokinetic curves, it was evident, that, in agreement with the data of literature [Kleinbloesem et al. 1993, Schall et al. 1994], food ingestion increased the relative bioavailability and maximum plasma concentration (Cmax). Considering the average of the parameter values and also the respective statistical tests, it was also apparent that the time to maximum plasma concentration (tmax), the mean residence time (MRT), and the half-value duration (HVD) all decreased significantly upon the effect of food ingestion.

Automated determination of levodopa and carbidopa in plasma by high-performance liquid chromatography-electrochemical detection using an on-line flow injection analysis sample pretreatment unit.

An automated analytical procedure is described for the parallel determination of L-3,4-dihydroxyphenylalanine (levodopa, L-dopa, LD) and the analogous hydrazine compound carbidopa (CD) in dog plasma by ion-pair high-performance liquid chromatography with electrochemical detection (HPLC-ED). After deproteinization of the plasma samples with perchloric acid the catecholamines were extracted from the supernatant by adsorption on a small column filled with alumina. The extraction and redissolution were automatically performed in a flow injection analysis unit (FIA) coupled to the HPLC system. The performance of the whole system was tested on dog plasma samples including specimens taken after oral administration of the anti-Parkinsonism drug Duellin, which is a combination tablet of levodopa and carbidopa.

Human bioequivalence study of a new nifedipine containing retard filmtablet after single and repeated administration.

A clinical pharmacokinetic bioequivalence study with two retard filmtablet preparations, both containing 20 mg of nifedipine (CAS 219829-25-4) was carried out. The investigated test preparation was Cordaflex 20 mg retard filmtablet. The pharmacokinetic parameters were determined after single and repeated administration in 15 and 16 healthy male volunteers, respectively, in open, randomised studies of cross-over design. Plasma levels of nifedipine were determined by HPLC with electrochemical detection using a robotic sample preparation technique. Statistical comparison of the pharmacokinetic parameters (AUC0-infinity, AUCss, tau tmax, Cmax, Css,min, Css,av, MRT, etc.) calculated from plasma concentration-time curves by ANOVAlog, confidence interval, Schuirman's, Westlake's, Anderson's and Wilcoxon's tests, furthermore the comparison of the clinical results did not show any significant difference between the two preparations. It is concluded that the two preparations are bioequivalent after repeated administration.

Sensitive high-performance liquid chromatographic determination of nifedipine in dog plasma using an automated sample preparation system with laboratory robot.

Nifedipine, a calcium-channel blocking drug was analysed in dog plasma after oral dosing with two different formulations. Sample preparation was automated with a laboratory robot. Quantitative determination of the drug was performed on a reversed-phase HPLC system with electrochemical detection (ED) using an internal standard. Validation of the analytical method showed that the system is well suited for pharmacokinetic studies on dogs. The assay was linear in the range 1-50 ng/ml. Inter-day and intra-day variability were between 6.43-18.15% C.V. and 1.57-5.53% C.V., respectively.

[Comparative human pharmacokinetic studies of 20 mg nifedipine-containing Cordaflex and Adalat film coated retared tablets]. / Cordaflex és Adalat 20 mg filmtabletták összehasonlító humán farmakokinetikája egyszeri és ismételt dozírozás után.

Comparative pharmacokinetic studies have been carried out with two 20 mg nifedipine active substance-containing retard film coated tablets, Cordaflex produced by EGIS Pharmaceuticals Co., Ltd. and Adalat of Bayer AG. The pharmacokinetic parameters and the relative bioavailability were determined in 15 and 16 healthy male volunteers, respectively after single and repeated administration in open, randomized cross over study. The plasma concentration of nifedipine was determined by HPLC-ED method, using laboratory robot for automated sample preparation. On the basis of graphical and statistical comparison of the pharmacokinetic parameters (AUC0-infinity, AUCss,0-tau, tmax, Cmax, Css,min, Css,av, MRT, etc.) calculated from the time-plasma concentration curve, moreover on the basis of clinical results, there was no significant difference between the two preparations. In conclusion, the relative bioavailability of Cordaflex and Adalat 20 mg retard tablets did not show significant difference after single and repeated administration.

Flow immunoassay using solid-phase entrapment.

A flow injection immunoassay was performed using a column packed with reversed-phase sorbents to effect separation of the immunoreacted species by entrapping free analyte and allowing antibody-conjugated analyte to pass unretained. Fluorescein-labeled analyte was measured in a competitive assay for the anticonvulsant drug phenytoin. The simplicity of the assay was the greatest advantage of the technique, which allowed for measurement of phenytoin in a 2-min assay time. The reliable detection limit for the assay was 5 nmol L(-)(1) of phenytoin in serum. The columns were regenerated with periodic injections of ethanol solutions to remove the entrapped analyte and prepare the column for subsequent analyses.

[Lymphoepithelial carcinoma of the nasolacrimal duct--a case report]. / Das lymphoepitheliale Karzinom des Ductus nasolacrimalis-Bericht über einen Fall.

PATIENT: A 59-year-old patient with an extremely rare lymphoepithelioma of the nasolacrimal duct was treated with radiation therapy and chemotherapy. RESULTS: A complete remission was achieved. Serious side effects and late effects have, until now, not been observed. The patient has been free of tumor for two and a half years. CONCLUSIONS: With this case report, the authors wish to draw the attention of otorhinolaryngologists to this unusual tumorsite. They emphasize that good results can be achieved with a combination of radiation therapy and chemotherapy, if surgery is not possible.

Selective ion-exchanger behaviour of neutral carrier ion-selective electrode membranes.

Radiotracer experiments confirm that neutral carrier ion-selective electrodes made from plasticized PVC are low-capacity selective ion-exchangers. The ion-exchange selectivity is closely correlated to potentiometric selectivity.