Techno-economic and environmental approaches of Cd2+ adsorption by olive leaves (Olea europaea L.) waste.

In this study, the techno-economic approach of olive leaves (Olea europaea L.) wastes for the removal of Cd2+ from aqueous solutions was demonstrated. The adsorption process was illustrated regarding batch experiments and scanning electron microscopy, energy dispersive X-ray, and Fourier-transform infrared characterization. The optimum pH and contact time were 6.6 and 123 min, respectively, giving Cd2+ removal efficiencies of 94.9% at Co = 50 mg/L and 81.5% at Co = 100 mg/L. The monolayer adsorption capacity of the Langmuir isotherm model was 32.6 mg/g (R2 = 0.97). The adsorption mechanisms might be related to (a) ion exchange with cations (e.g., K+, Na+, and Ca2+), (b) formation of cadmium chloride complexes, (c) interaction with oxygen-containing functional groups, (d) physical agglomeration in the pore surface, and (e) precipitation interaction using inorganic minerals (i.e., carbonates, phosphates, and silicates). The total cost of the adsorption process for the treatment of ions-containing wastewater was 0.038 $USD/m3. Assuming a benefit-cost of tertiary treated water as 0.044 $USD/m3, the adsorption system could attain a payback period of 5.7 years. This period was shorter than the lifetime of the capital investment (i.e., 10 years), and hence, the project would be economically feasible for an application.

An intervention significantly improve medical waste handling and management: A consequence of raising knowledge and practical skills of health care workers.

OBJECTIVE: Health-care waste has not attended much attention in developing countries. Staff is involved in cleaning and collect waste may often be at greater risk due to their less education and training. The current intervention study was conducted to improve knowledge and practice skills for medical waste handlers in some selected hospitals of Alexandria, Egypt. METHODS: An intervention study was conducted on medical waste handlers from some selected hospitals who accepted to participate in the study in the period of May 2015 to June 2016. A predesigned questionnaire was developed to measure knowledge, skills, and practice on medical waste management pre- and post-intervention. RESULTS: Analysis of pre-intervention data revealed that 9.6%, 80.8%, and 9.6% of participants had high, moderate, and low knowledge levels, respectively. Whereas post-intervention, data revealed that 97.3%, 2.2%, and 0.5% of workers had high, moderate, and low knowledge levels, respectively. A significant increase in knowledge after the intervention was detected among all knowledge items except in four items which were related to the necessity to segregate medical waste, knowledge of color coding system for segregation, disposal of general waste in black bags and disposal of infectious waste in red bags. Regarding practice of waste handlers, 80% were in poor practice category pre-training and changed to 0.8% post-training; 1.1% were in good practice category and increased to 92.1% post-training. CONCLUSION: The current findings emphasize the role of educative skill-raising training in enhancing knowledge and practice skills of medical waste handlers.

TP53 mutations in circulating free DNA from Egyptian patients with non-Hodgkin's lymphoma.

BACKGROUND: P53 protein plays important role in the maintenance of genome stability in mammalian cells; it acts in many processes including cell-cycle checkpoint, DNA repair, apoptosis, and angiogenesis. Mutations of P53 have been reported as common mutations in solid tumours, including non-Hodgkin lymphoma, NHL, and have been implicated in drug resistance, aggression and poor prognosis. Chronic infection with hepatitis C, HCV, has been associated in some studies with increased risk of NHL. HCV is a widespread infection in the Egyptian population. Circulating free DNA (CFDNA) has been shown to be a good source of liver tissue-derived DNA in African and Asian patients with chronic liver disease or hepatocellular carcinoma, HCC. Our previous results have shown TP53 mutations in 5% of CFDNA and 10% of tumours of HCC, with underlying HCV. OBJECTIVE: Since previous studies have shown p53 mutations in the DNAs extracted from the NH lymphoid tumours, we have assessed the presence of p53 mutations from exons 5 to 9 in CFDNA in patients with NHL, from Alexandria, Egypt, where HCV is highly prevalent, in a first attempt case-control preliminary study. METHODS: CFDNA was extracted from sera of 20 cases with NHL and 20 negative control individuals. The retrieved serum DNAs were screened for TP53 mutations from exons 5 to 9 using direct sequencing and a PCR-restriction digestion analysis (RFLP). Concentrations of CFDNA were measured using Fluorometric assay. RESULTS: Concentrations of CFDNA were significantly higher among NHL patients compared to the negative control individuals indicating a very high release or turn-over of DNA from the tumour into the blood stream among NHL patients. Mutations of p53 determined in NHL cases (30%) were of Arg-176 (1/20: 5%), Phe-238 (1/20: 5%), Ser-249 (2/20; 10%), Lys-249 (1/20: 5%) and Phe-250 (1/20: 5%). No mutations were detected among controls. However, Arg-213 polymorphism was found in 2 cases of NHL (10%) and in 1 case of controls (5%). CONCLUSION: Our findings of higher DNA concentrations with some p53 mutations in CFDNA from patients with NHL that match the previous reported p53 mutations from tumour DNA may hold promises that CFDNA may serve as a convenient source of tumour-derived DNA to serve as a promising tool of a non-invasive, low-cost new strategy for earlier detection, diagnosis and follow up of the disease. A large-scale prospective study utilizing CFDNA and DNA from tumours of NHL patients will be required to validate this first trial of utilizing CFDNA from NHL patients.

Ser-249 TP53 and CTNNB1 mutations in circulating free DNA of Egyptian patients with hepatocellular carcinoma versus chronic liver diseases.

Circulating free DNA (CFDNA) has been shown to be a good source of liver tissue-derived DNA in African and Asian patients with chronic liver disease or HCC. In Egypt, HCC is a frequent carcinoma and mostly occur in the context of chronic infection by HCV, a widespread infection in the Egyptian population. Here we have examined the presence of mutations in TP53 at codon 249 (Ser-249, considered as a hallmark of mutagenesis by aflatoxin) and in CTNNB1 (gene encoding beta-catenin) in CFDNA of patients with HCC or chronic liver disease, from Alexandria, Egypt. The DNA concentrations were significantly higher in HCC patients compared to HBV and HCV carriers without cancer, and to sero-negative individuals. Ser-249 TP53 mutations were determined using PCR-restriction digestion (RFLP) in CFDNA of 255 subjects, and confirmed by sequencing. Ser-249 was found in CFDNA of 12 subjects (4.8%), with the highest prevalence in subjects with chronic liver disease and infection by HBV (6/36; 16.7%) Mutations in CTNNB1 were examined using PCR combined to DHPLC and followed by sequencing. No mutations were found in CTNNB1 neither in CFDNA or in tumour tissue. In parallel, studies on DNA extracted from 20 HCC biopsies showed the presence of ser-249 mutation in two cases (10%). These results indicate that mutagenesis by aflatoxin may play a role in hepatocarcinogenesis in Egypt, and CFDNA may serve as a convenient source of material in monitoring the effects of aflatoxin exposure and viral infections in chronic liver disease and cancer.

Assessment of asbestos in drinking water in alexandria, egypt.

Over the past several years, the presence of fibrous asbestos particulates has been observed in a number of municipal water supplies throughout the USA, Canada, and several other regions all over the world. The possible health hazards which these fibers present have spurred a great deal of interest in the problems of detection and removal of the submicroscopic particulates in water. Asbestos is a group of fibrous metamorphic silicate minerals that is ubiquitous in the environment as a result of its extensive industrial use and the dissemination of fibers from natural sources. The health hazards associated with inhalation of asbestos in the occupational environment have long been recognized including asbestosis, bronchial carcinoma, malignant mesothelioma of the pleura and peritoneum, and possibly cancers of the gastrointestinal tract and larynx. It is introduced into water by the dissolution of asbestos-containing minerals and ores, and from industrial effluents, atmospheric pollution and erosion of asbestos-cement (A/C) pipes in the distribution systems of drinking water. In Alexandria, most of the pipes in the distribution systems of drinking water are asbestos-cement (A/C) pipe system. Drinking water samples (1 liter each) were collected in glass containers from different regions in Alexandria and filtered in cellulose filters (mixed cellulose ester type filters of pore size 0.2 mum) within less than 48 hours. Filters were allowed to dry, gold plated and scanned microscopically. Asbestos fibers were detected in all water samples collected from regions having A/C pipe drainage system. No fibers detected in regions, where the pipe distribution system was poly venyl pipe system or changed from A/C pipe to cast iron pipe system. The determination of asbestos fibers in drinking water of Alexandria should have particular concern because of the health hazards that might be associated with their presence.

A comparative study on the medical waste disposal in some hospitals in Alexandria.

Though healthcare services aim to reduce the health problems and prevent the potential risks to the health of the community. These services create wastes which are considered as hazardous materials due to the higher potential of infection and injury possessed by these wastes than any other type of waste. Healthcare waste management is an integral part of healthcare services, and can create harm through inadequate waste management; thus reducing the overall benefits provided by healthcare centers. In the current study, a survey for medical waste disposal was performed in order to examine the current status of medical waste disposal in some hospitals in Alexandria and to properly assess management of this type of hazardous waste. A questionnaire was designed for hospitals to assess the quantity of medical waste, collection, sorting, storage, transportation and way of final disposal. From the total waste generated by healthcare activities, almost 80% are waste similar to domestic waste. The remaining approximate of 20% is considered as hazardous waste. As Alexandria has about 3911 healthcare facilities providing medical services for people, a huge amount of medical waste are generated daily with about 208 tons generated per month. The results revealed that the most common problems associated with healthcare wastes are the absence of waste management, lack of awareness about their health hazards, insufficient financial and human resources for proper management, and poor control of waste disposal. The current situation of medical waste disposal in Alexandria is depending on incinerators. Some of these incinerators are not working anymore. Incinerations as a system is not accepted at the time being in most developed countries due to the risks associated with it and suitable substitution management system for medical waste disposal is now taking its place.

A prediction model for the incidence patterns of female breast cancers in Alexandria, Egypt.

Breast cancer is the most common cancer among women in the world. Female breast cancer incidence has historically increased all over the world during the past decades. The purpose of this study was to estimate the patterns of female breast cancer incidence in Alexandria, Egypt, from 1972 to 2001. An up-to-date perspective systematically collected data on the incidence of female breast cancers was used. Age categories-estimated incidence over the 30 years period were computed. Time series analysis showed that incidence of breast cancers is increasing significantly during the studied period. Linear trend model was best fitted, with the studied interval and the estimated cancer cases per year taken into account. Age-incidence correlation showed a high correlation between age group of 40-50 years old and incidence. Analysis of the patterns for cancer incidence indicates that the highest occurrence of female breast cancer is shown in the age category of 45-50 years followed respectively by the age categories of 40-45 years, 50-55 years, 35-40 years and then 55-60 years. These findings support the growing body of evidence from female breast cancer incidence suggesting that age is a well-known risk factor for breast cancer susceptibility.

Patterns in the incidence of pediatric cancer in Alexandria, Egypt, from 1972 to 2001.

Cancer among children is a substantial public concern. The epidemiology of childhood cancer is not fully understood. The descriptive epidemiology for cancer incidence has been a subject of considerable debate in order to identify and clarify the role of etiological factors and environmental exposure in cancer. An up-to-date perspective systematically collected data on the incidence of childhood cancer in Alexandria was used. Statistical analysis revealed that the over all cancer incidence rate increased from 1972 to 2001. The incidence of lymphatic and haemopoietic cancers increased dramatically over the specified period. The lymphatic and haemopoietic ancer incidence in 2001 have increased approximately 11 times cancer incidence in 1972. The incidence of leukemia among infant less than 5 years increased exponentially with a higher incidence among boys. Little increase was observed in the cancers of oral cavity, digestive organs and respiratory system over the specified period. Incidence of genito-urinary cancers have increased 3.7 times cancer incidence in 1973. Brain and central nervous system cancers showed an increase of 4.5 fold during the 30 years studied period. The trends in some cancer types suggest the need of a closer examination of the underlying factors and environmental contaminants leading to the disease in children.