ABSTRACT
Anopheline mosquitoes are responsible for transmission of some diseases such as malaria. This study was carried out in five villages of Dashtestan county, Bushehr province, south of Iran with mountainous and plain areas. Anopheles larvae were sampled once a month from May to July 2021 by dipping method using standard dippers. Adults were captured by the total catch technique. In this study, 1062 Anopheles mosquitoes were collected including 850 adults and 212 larvae. Samples were A. superpictus, A. stephensi, A. dthali and A. fluviatilis. The dominant species at all sites (larvae and adults) were A. dthali (31.35%), A. superpictus (28.93%), A. stephensi (27.77%), and A. fluviatilis (11.95%), respectively. Among adults, A. stephensi was the most frequent species, but among larvae, A. dthali was the dominant species. The highest number of A. dthali was captured from Dalaki village with 35%, while A. superpictus and A. fluviatilis were not caught at this station. The minimum collected adults of all species occurred in Bashirabad. This result showed that A. stephensi plays an important role in south Iran, which is under the elimination phase. The fauna and niches of Anopheles has different patterns depending on ecological, climatic, and topographic features. These items affect host preferences, feeding behaviors, and distribution of these species. A monthly or annual entomological survey is necessary in regions with mobile populations since imported malaria is a problematic issue in the elimination programs of Iran.
ABSTRACT
Background: Malaria has remained the most dreadful vector-borne disease; hence, vector control is the most affordable and achievable approach to mitigate the disease burden. Due to the emergence of resistance and environmental pollution, herbal larvicides are considered an alternative to chemical types. Also, nanotechnology has been proposed as a promising solution to improve the efficiency of plant larvicides. This study aimed to develop an effective herbal larvicide. Methods: The chemical composition of Elettaria cardamomum essential oil (EO) was first investigated. Nanoliposomes containing the EO were then prepared using the ethanol injection method. After that, the larvicidal efficacy of the EO and its liposomal state were compared against Anopheles stephensi in laboratory conditions. Results: Alpha-terpinyl acetate (77.59%), eucalyptol (4.38%), nerolidol (2.96%), linalool (1.77%), and limonene (1.69%) were the five major compounds of the EO. Nanoliposomes containing the EO with a particle size of 73±5 nm and a zeta potential of -16.3±0.8 mV were prepared. Additionally, the ATR-FTIR analysis verified the successful loading of the EO into nanoliposomes. The larvicidal activity of nanoliposomes exhibited remarkable potency, with an LC50 value of 14.35 (10-18) µg/mL, significantly more potent than the non-formulated EO, which had an LC50 value of 33.47 (28-39) µg/mL against Anopheles stephensi larvae. Conclusion: The nanoliposomes containing E. cardamomum EO showed promising efficacy against An. stephensi larvae. It could thus be considered for further application against other species of mosquitoes.