Synthesis, characterization, and cytotoxicity studies of nanocellulose extracted from okra (Abelmoschus Esculentus) fiber.

Nanocellulose, especially originating from a natural source, has already shown immense potential to be considered in various fields, namely packaging, papermaking, composites, biomedical engineering, flame retardant, and thermal insulating materials, etc. due to its environmental friendliness and novel functionalities. Thus, a thorough characterization of nanocellulose is a hot research topic of research communities in a view to judge its suitability to be used in a specific area. In this work, a kind of green and environment-friendly nanocellulose was successfully prepared from okra fiber through a series of multi-step chemical treatments, specifically, scouring, alkali treatment, sodium chlorite bleaching, and sulfuric acid hydrolysis. Several characterization techniques were adopted to understand the morphology, structure, thermal behavior, crystallinity, and toxicological effects of prepared nanocellulose. Obtained data revealed the formation of rod-shaped nanocellulose and compared to raw okra fiber, their size distributions were significantly smaller. X-ray diffraction (XRD) patterns displayed that compared to the crystalline region, the amorphous region in raw fiber is notably larger, and in obtained nanocellulose, the crystallinity index increased significantly. Moreover, variations in the Fourier transform infrared spectroscopy (FTIR) peaks depicted the successful removal of amorphous regions, namely, lignin and hemicelluloses from the surface of fiber. Thermostability of synthesized nanocellulose was confirmed by both Differential Scanning Calorimetry (DSC) analysis, and thermogravimetric analysis (TGA). Cytotoxicity assessment showed that the okra fiber-derived nanocellulose exhibited lower to moderate cellular toxicity in a dose-dependent manner where the LD50 value was 60.60 µg/ml.

Acceptability, cost-effectiveness, and capacity of a facility-based seasonal influenza vaccination among high-risk groups: a study protocol in selected tertiary care hospitals of Bangladesh.

BACKGROUND: In Bangladesh, seasonal influenza imposes considerable disease and economic burden, especially for those at high-risk of severe disease. The most successful approach for influenza prevention is the administration of a vaccine. Many poor and middle-income nations, including Bangladesh, do not have a national strategy or program in place for seasonal influenza vaccines, despite the World Health Organization's (WHO) advice to prioritize high-risk populations. Additionally, there is a scarcity of substantial data on the cost-effectiveness of seasonal influenza vaccination in these countries. The aim of our study is to determine acceptability, health beliefs, barriers, and intention of receiving influenza vaccine among high-risk populations, assess the cost-effectiveness of implementing a facility-based seasonal influenza vaccination programme, and investigate the required capacity for a potential seasonal influenza vaccination programme. METHODS: We will undertake this study following STROBE guidelines. We will conduct the study in inpatient and outpatient departments of three selected tertiary-level hospitals leveraging the ongoing hospital-based influenza surveillance (HBIS) platform. The study population will include the WHO-defined four high-risk groups excluding healthcare workers: children six months to eight years, pregnant women, elderly ≥ 60 years, and adults with chronic diseases. We will collect quantitative data on participants' acceptability, health beliefs, barriers, and vaccination intentions using the health belief model (HBM) from patients meeting the criteria for high-risk populations attending two public tertiary-level hospitals. In one of the two public tertiary-level hospitals, we will arrange an influenza vaccination campaign before the influenza season, where the vaccine will be offered free of cost to high-risk patients, and in the second hospital, vaccination will not be offered. Both the vaccinated and unvaccinated participants will then be followed-up once a month for one year to record any influenza-like illness, hospitalization, and death. Additional data for objective two will be collected from patients with symptoms of influenza-like illness (ILI) and severe acute respiratory infection (SARI) at one public and one private hospital to determine both direct and indirect costs associated with influenza illness. We will estimate the required number of influenza vaccines, safe injections, and total storage volume utilizing secondary data. We will use a deterministic Markov decision-analytic model to estimate the cost-effectiveness of facility-based influenza vaccination in Bangladesh. DISCUSSION: The results of this study will enable the National Immunization Technical Advisory Group and the Ministry of Health & Family Welfare of Bangladesh to decide what steps to take to develop and implement an influenza vaccination strategy targeting high-risk populations. TRIAL REGISTRATION: The Clinicaltrials.gov registration number is NCT05996549. The registration for the protocol version 2.0 took place in August 2023, with the initial participant being enrolled in March 2022.

Cost of Acute and Sequelae Care for Japanese Encephalitis Patients, Bangladesh, 2011-2021.

Japanese encephalitis (JE) is associated with an immense social and economic burden. Published cost-of-illness data come primarily from decades-old studies. To determine the cost of care for patients with acute JE and initial and long-term sequelae from the societal perspective, we recruited patients with laboratory-confirmed JE from the past 10 years of JE surveillance in Bangladesh and categorized them as acute care, initial sequalae, and long-term sequelae patients. Among 157 patients, we categorized 55 as acute, 65 as initial sequelae (53 as both categories), and 90 as long-term sequelae. The average (median) societal cost of an acute JE episode was US $929 ($909), of initial sequelae US $75 ($33), and of long-term sequelae US $47 ($14). Most families perceived the effect of JE on their well-being to be extreme and had sustained debt for JE expenses. Our data about the high cost of JE can be used by decision makers in Bangladesh.

Digitalized to reach and track: a retrospective comparison between traditional and conditional estimate of vaccination coverage and dropout rates using e-Tracker data below one-year children in Bangladesh during-COVID and pre-COVID period.

Background: With an impressive track record in expanding childhood immunization and an inclination to adopt digitalization in healthcare service delivery, Expanded Program on Immunization (EPI) Bangladesh piloted the e-Tracker intervention in Moulvibazar district and Dhaka South City Corporation (Zone-5) from 2019 till the end of 2021. Methods: We retrieved and analyzed the digitalized e-Tracker data of 114,194 infants born between January 1, 2019 and December 31, 2020, with help from Health Management Information System (HMIS) and UNICEF Bangladesh. Childhood vaccination coverage and dropout rates were determined using a 'Traditional approach' traditionally used by WHO and a 'Conditional technique' with a modified denominator. Using a multiple logistic regression model, we examined the effects of COVID-19, birth-cohorts, mother education, and location on vaccination rates (coverages & dropouts) to aid with informed decision-making by the policymakers. Findings: The conditional estimation method yielded a lower full vaccination coverage during pre-COVID period than the national and global reported coverage derived using the 'traditional method' (73.4% vs. 89.0% & 81.0%). As expected, while the coverage has decreased, the dropout rate increased "during-COVID" compared to the "pre-COVID" period. However, dropouts were estimated lower in the 'conditional method.' The average age (in months) for getting BCG was higher in Moulvibazar (∼2.5 months) than that in Dhaka (∼1.4 months). All birth-cohorts from 'the during-COVID period had about 30% lower odds of getting fully vaccinated than those from the 'pre-COVID' period. Interpretations: Age-cohort-specific analysis showed a decline in coverage rates before and during COVID, but e-Tracker didn't have enough data to draw additional conclusions. The server only stored the child's gender, the caregiver's monthly salary, and the mother's education. It didn't track any other factors related to dropout rates. The e-Tracker is an excellent tool for measuring real coverage and should be scaled nationwide. Funding: UNICEF, Bangladesh.

Flame Retardant Coatings from Bio-Derived Chitosan, Sodium Alginate, and Metal Salts for Polyamide 66 Textiles.

Bio-derived polysaccharides, namely, chitosan (CS) and sodium alginate (SA) were considered in a layer-by-layer (LbL) deposition to construct flame retardant coatings onto the polyamide 66 (PA66) fabric surfaces. The as-prepared coatings were further modified in the impregnation process with a number of inorganic salts containing boron, nickel, and iron elements. Obtained results revealed that the simultaneously LbL-assembled and metal salt-treated fabric samples exhibited superior flame retardant performance compared to the only LbL-deposited fabric samples. The limiting oxygen index (LOI) value reached up to 25.5% of the CS-SA-iron salt treated fabric sample and the dripping tendency was completely diminished only for the LbL-metal salt modified fabric samples. Among the treated fabric samples, the CS-SA-iron-salt-modified fabric sample exhibited a maximum reduction in the peak heat release rate by 34% and handed improved and higher quality char residues, indicating a possible condensed phase flame retardant mechanism of this applied finishing. Moreover, metal salt-induced cross-linking could enhance the coating stability and durable finishes against regular home laundering where an iron-salt-treated fabric sample could retain anti-dripping properties even up to 10 laundering cycles. Thus, this pairing of bio-macromolecules (i.e., charring agent) with the metal salts in a hybrid system showed efficacy in improving the fire performance of polyamide textiles via the synergistic involvement between them.