ABSTRACT
Pickering emulsions provide a promising platform for the efficient delivery of bioactive. However, co-delivery of fragile bioactives with different physicochemical properties for comprehensive effects still faces practical challenges due to the limited protection for bioactives and the lack of stimuli-responsive property for on-demand release. Herein, a stimuli-responsive co-delivery system is developed based on biomineralized particles stabilized Pickering emulsions. In this tailor co-delivery system, hydrophilic bioactive (pepsin) with the fragile structure is encapsulated and immobilized by biomineralization, the obtained biomineralized particles (PPS@CaCO3) are further utilized as emulsifiers to form O/W Pickering emulsions, in which the hydrophobic oxidizable bioactive (curcumin) is stably trapped into the dispersed phase. The results show that two bioactives are successfully co-encapsulated in Pickering emulsions, and benefiting from the protection capacities of biomineralization and Pickering emulsions, the activity of pepsin and curcumin shows a 7.33-fold and 144.83-fold enhancement compared to the free state, respectively. Moreover, In vitro study demonstrates that Pickering emulsions enable to co-release of two bioactives with high activity retention by the acid-induced hydrolyzation of biomineralized particles. This work provides a powerful stimuli-responsive platform for the co-delivery of multiple bioactive compounds, enabling high activity of bioactives for the comprehensive health effects.
ABSTRACT
Pickering interfacial biocatalysis (PIB) paves the way for efficient enzymatic catalysis in the biphasic system. However, the Pickering interfacial biocatalysts located on the oil-water interface still face the inevitable deactivation when one of the phases contains the reactant that inactivates the enzyme. Herein, the positioning of lipase-entrapped colloidosomes (LECs) at the emulsion interface is rationally designed by physically tuning the wettability, which allows LECs to protrude into the selected phase to protect the lipase away from the damage of the reactant. As a proof of concept, LECs with different positioning at the interface are used as Pickering interfacial biocatalysts to produce biodiesel by esterification of lauric acid and methanol. Impressively, the LECs that protrude into the oil phase possess an optimal catalytic performance to protect more lipases away from the damage of the reactant of short-chain alcohol, which shows an 8.18-fold enhancement in specific activity relative to the free lipase, reach a biodiesel yield of 80.37% after 8 h, and retain the 96.44% of relative activity after 10 cycles. This study provides a novel and robust platform for Pickering emulsion-enhanced biocatalysis.
