Pesticides in human milk collected from Jinhua, China: Levels, influencing factors and health risk assessment.

In China, many studies have been carried out on pesticide residues in human milk, yet all of them are on organochlorine pesticides (OCPs) and mostly focused on large, economically developed cities. In this study, 27 pesticides including OCPs, pyrethroid pesticides (PYRs) and organophosphate pesticides (OPPs) in human milk were investigated in Jinhua, an inland and medium sized city in China. Method based on QuEChERS extraction and gas chromatography-mass spectrometer (GC-MS) determination was adopted to analyze the above pesticide residues. The influencing factors as well as the health risks were also evaluated. Results show that PYRs and OPPs in human milk samples were both undetectable. Regarding OCPs, the detection rate of hexachlorobenzene (HCB), ß-hexachlorocyclohexane (ß-HCH) and p,p'-dichlorodiphenyl-dichloroethylene (p,p'-DDE) were 83.6%, 36.4% and 58.2%, respectively, and their mean value were 29.4, 32.0 and 85.2 ng/g lipid, respectively. p,p'-DDE levels in human milk was significantly (p < 0.05) related to maternal age, but no association was detected between OCPs residues and other factors (living environment, dietary habit, living style, etc.), suggesting that OCPs in human milk in Jinhua were originated from nonspecific source. All estimated daily intake of pesticides (EDIpesticides) by infants were under the guideline suggested by Food and Agriculture Organization (FAO) and China Ministry of Health (CMH). Yet 9% of EDIsHCB and 16% of EDIsHCHs exceeded the guideline recommended by Health Canada. The associations between DDE residues and the delivery way as well as HCBs residues and the birth weight were seemly significant, yet the significance disappeared when consider age or gestational age as a cofounder, indicating that OCPs residue in mother's body in Jinhua has no obvious influence on fetus development and the delivery way.

Pesticide residues in breast milk and the associated risk assessment: A review focused on China.

This review aims to provide an overview of studies on pesticide residues in breast milk in China and the related health risk to mother and infants. Results showed that the investigations of breast milk covered 22 provincial administrative regions of China. Beijing and some densely populated and economically developed areas have most publications. The study frequency was followed the order of DDTs>HCHs>HCB > ∑Drins,∑Chlordane. While the residue levels were ranked as DDTs, HCHs > ∑Drins>HCB > ∑Chlordane. The highest residue levels of DDTs and HCHs in breast milk were found in 1980s (~10,000 ng/g lipid), then experienced a sharp decrease in 1990s (~1000-2000 ng/g lipid). In 2000s and 2010s, DDTs, and HCHs residue still showed a decreasing trend. Spatially, people located in urban area, coastal areas and southern China tend to have higher pesticide residues as compared to rural area, inland area and northern China, respectively. Other factors such as dietary habit, living environment, the maternal age, the parity, body mass index, lactation period, menstruation characteristics as well as hormonal drug intake and infertility treatment will also affect the pesticide residues in breast milk of Chinese people. According to the estimated daily ingestion (EDI) of breast milk, the average health risk for infants were generally exceeded the acceptable level before 2006, while after that, most EDI values were within the standard. Body burden of pesticides in mother can also be evaluated by using the residue data in breast milk, but no relevant guidelines were available. Other knowledge gap included 1) for some provinces with large consumption of pesticides or located in remote and plateau areas, there are few/no studies available; 2) current study on pesticide residues in breast milk in China were only focused on organochlorine pesticides, research on current used pesticides (such as pyrethroids, organophosphorus, carbamate) were necessary in the future.

Is single-incision laparoscopic appendectomy suitable for complicated appendicitis? A comparative analysis with standard multiport laparoscopic appendectomy.

BACKGROUND: Despite emerging evidences on the feasibility and efficacy of single-incision laparoscopic appendectomy (SILA) for managing simple appendicitis, advancing its use for treating complicated appendicitis remains unwarranted. This study aimed to evaluate the surgical outcome of SILA compared with that of standard multiport laparoscopic appendectomy (MPLA) in the management of complicated appendicitis. METHODS: Between July 2013 and September 2017, 40 patients who underwent SILA and 150 patients who underwent MPLA for the treatment of complicated appendicitis, defined as AAST grades II-V, were recruited for this study. The demographic characteristics, intraoperative and recovery parameters were retrospectively recorded. No routine drainage was placed in the SILA group. RESULTS: SILA was smoothly performed without requiring conversion to MPLA. No significant difference was observed between the 2 groups in demography and preoperative severity. The operation duration was approximately 8 min shorter in the SILA group than in the MPLA group, without a statistical difference (60.03 ± 21.01 vs. 68.04 ± 32.03 min, p = 0.222). The recovery parameters, including soft diet intake and postoperative hospitalization, were more favorable in the SILA group (p < 0.001). Despite the absence of routine drainage in the SILA group, only 1 patient developed an intra-abdominal hematoma necessitating further computed tomography-guided drainage. CONCLUSION: SILA is safe and feasible for the management of complicated appendicitis. SILA had non-inferior results to MPLA in terms of time to resume oral intake and postoperative hospital stay. Furthermore, this study posted a question on the concept of routine postoperative drainage in complicated appendicitis.

Arsenic species analysis by ion chromatography-bianode electrochemical hydride generator-atomic fluorescence spectrometry.

In this work, a novel hyphenated technique ion chromatography-bianode electrochemical hydride generator-atomic fluorescence spectrometry (IC-BAECHG-AFS) system was developed for on-line simultaneous analysis of arsenite (As(III)), dimethylarsinic acid (DMA), monomethylarsonic acid (MMA), and arsenate (As(V)). Parameters that might affect hydride generation efficiency were investigated and the interference of various ions was also examined. Under the optimal conditions, the hydride generation efficiency of bianode electrochemical hydride generator (BAECHG) was close to the hydride generation efficiency of chemical hydride generator (CHG). The linear ranges were 5-200 microg L(-1) for As(III), DMA and MMA and 10-200 microg L(-1) for As(V). The limits of detection (LOD, S/N=3) were 3.04, 4.27, 3.97 and 9.30 microg L(-1) for As(III), DMA, MMA and As(V), respectively. The relative standard deviations (RSD, n=7) of As(III), DMA, MMA and As(V) were 4, 3, 5 and 4%, respectively. The proposed method was also applied to the determination of the four arsenic species in dental pulp inactivative agent (type I). The recoveries of the four arsenic compounds were in the range of 91.6-103.2%.