Ethnoveterinary survey of trypanocidal medicinal plants of the beninese pharmacopoeia in the management of bovine trypanosomosis in North Benin (West Africa).

Cattle breeding is of great socio-economic importance for Benin's cattle farmers in general and those of North Benin in particular. The objective of this study is to inventory the natural products of medicinal plants of the Beninese pharmacopoeia for the management of trypanosomes in cattle in North Benin. The methodology consisted of individual and semi-structured interviews with cattle farmers on the use of medicinal plants. A total of 360 cattle farmers were selected and interviewed in twelve villages in four municipalities (Tchaourou, N'dali, Bembèrèkè and Gogounou) in northern Benin. Different quantitative ethnobotanical indices were calculated to determine the level of use of plant species. The Relative Frequency of Citation (RFC), the Informant Consensus Factor (ICF = 0.918) and the Generic Coefficient (Rg = 1.04) were evaluated. The knowledge of medicinal plants was influenced by the level of education and the main activity of those who practiced animal husbandry. The results yielded 48 medicinal plants for veterinary use belonging to 46 genera and 28 families. The Leguminosae family (12.50%) was the most represented. The most cited plants with a RFC above 10% were K. senegalensis, P. africana, K. africana, M. inermis, S. latifolius, M. polyandra. The parts used were leaves (46.15%); barks (24.62%) and roots (15.38%). Decoction (53.23%), plundering (32.26%) and maceration (11.26%) were the main methods of preparation. The administration was mainly by oral route. The calculated indices show a high diversity of medicinal plants with trypanocidal properties in the control of cattle trypanosomosis in the Sudanese and Sudano-Guinean zones of northern Benin. Plant species with high citation and RFC values should be selected for comprehensive pharmacological and phytochemical research to validate this ethnomedical knowledge in the management of cattle trypanosomosis.

Typology of rabbit rearing systems in tropical areas: A case of Benin (West Africa).

The breeding of rabbits in tropical areas is confronted with enormous difficulties related to feeding and health, which limit the expansion and viability of these farms. The objective of this study is to carry out a typology of rabbit farms in tropical conditions to characterize the structure and function of this rearing in order to better understand their production results. A sample of 600 rabbit farms located throughout Benin was selected. The typology was carried out using multiple correspondence analysis (MCA) followed by Hierarchical Cluster Analysis (HCA) according to the Ward's aggregation algorithm and Euclidean distance, which identified five typological groups. Group 1 covered 45.7% of the farms and included small-scale production (fewer than 20 does) by professional breeders using traditional parasite control methods. Group 2 covered 3.3% of the rearing and includes a larger number of semi-extensive farms using self-produced feed. Group 3 (14.7%) was characterized by farms with fewer than 20 does that were semi-extensive and used more phytotherapy. In Group 4 (9.7% of the farms), the extensive mode was the most used, with veterinary medicine being the most used. Group 5 concentrated 26.7% of the farms and was marked by semi-extensive breeding. These farms did not record any cases of parasitosis. The typology carried out allowed a better understanding of the operating modes of these farms, their problems and the main limiting factors.

Anthelmintic Activity, Cytotoxicity, and Phytochemical Screening of Plants Used to Treat Digestive Parasitosis of Small Ruminants in Benin (West Africa).

Medicinal plants continue to be used alone or in combination with veterinary drugs to treat animal ailments, especially in developing countries where livestock farmers often lack access to modern veterinary services and drugs. In addition, digestive parasitosis remain a major constraint for small ruminant livestock. The objective of this study was to screen the anthelmintic activity of the main plants used in the treatment of the digestive parasitosis of small ruminants in Benin. A total of 40 extracts were prepared using the successive maceration of 10 plants in four solvents of increasing polarity. The phytochemical screening of the plants was performed, and the anthelmintic activity of the extracts was evaluated on L3 larvae of Haemonchus contortus. The cytotoxicity of the 40 extracts was determined on WI38 noncancerous fibroblast cells using the MTT assay, and the total phenol content (TPC), total flavonoid content (TFC), and condensed tannin content (CTC) were quantified in the most effective extracts using colorimetric methods. The results show that the plants contained tannins, flavonoids, and triterpenoids which may, in part, justify their anthelmintic activities. All plants gave active extracts at the highest concentration tested (1200 µg/mL). Methanol (MeOH) extracts were, in general, more effective than the hexane (HEX), dichloromethane (DCM), and aqueous (H2O) ones in inhibiting larval migration, with the MeOH extracts of Terminalia leiocarpa, Adansonia digitata, and Momordica charantia being the most effective. Nevertheless, the MeOH extract of M. charantia was highly cytotoxic at the concentration of 100 µg/mL. The anthelmintic activity of M. charantia, Vitex doniana, and Caesalpinia bonduc was studied on H. contortus for the first time. These results provide scientific information that can be used for better valorization of the anthelmintic potential of the studied plants and to initiate the process of the identification of new anthelmintic molecules.

Fractionation Coupled to Molecular Networking: Towards Identification of Anthelmintic Molecules in Terminalia leiocarpa (DC.) Baill.

Terminalia leiocarpa is a medicinal plant widely used in ethnoveterinary medicine to treat digestive parasitosis whose extracts were shown to be active against gastrointestinal nematodes of domestic ruminants. The objective of our study was to identify compounds responsible for this activity. Column fractionation was performed, and the activity of the fractions was assessed in vitro on Haemonchus contortus and Caenorhabditis elegans as well as their cytotoxicity on WI38 fibroblasts. Two fractions were the most active on both nematode models and less cytotoxic. LC-MS/MS analysis and manual dereplication coupled to molecular networking allowed identification of the main compounds: ellagic acid and derivatives, gallic acid, astragalin, rutin, quinic acid, and fructose. Other potentially identified compounds such as shikimic acid, 2,3-(S)-hexahydroxydiphenoyl-D-glucose or an isomer, quercetin-3-O-(6-O-galloyl)-ß-D-galactopyranoside or an isomer, and a trihydroxylated triterpenoid bearing a sugar as rosamultin are reported in this plant for the first time. Evaluation of the anthelmintic activity of the available major compounds showed that ellagic and gallic acids were the most effective in inhibiting the viability of C. elegans. Their quantification in fractions 8 and 9 indicated the presence of about 8.6 and 7.1 µg/mg ellagic acid and about 9.6 and 2.0 µg/mg gallic acid respectively. These concentrations are not sufficient to justify the activity observed. Ellagic acid derivatives and other compounds that were found to be positively correlated with the anthelmintic activity of the fractions may have additive or synergistic effects when combined, but other unidentified compounds could also be implicated in the observed activity.

Ethnoveterinary knowledge of sheep and goat farmers in Benin (West Africa): effect of socioeconomic and environmental factors.

Sheep and goats are two of the main animal species raised in Benin and one of the main sources of income for people living in rural areas. Faced with the inaccessibility of synthetic veterinary products and their low purchasing power, the majority of breeders use ethnoveterinary practices to treat small ruminants diseases. The specific objectives of the current study were (1) to document the traditional knowledge regarding the disorder groups treated and the medicinal plants used in the health and zootechnical management of small ruminants in Benin and (2) to assess the effect of gender, ethnicity, agro-ecological zone and herd size associated with them. To achieve these objectives, an ethnoveterinary survey was conducted in different agro-ecological zones from September 2018 to February 2019. A questionnaire was administered to 506 breeders. The data were analyzed through calculation of the Fidelity Level (FL), Cultural Importance Index (CI) and Informant Consensus Factor (ICF). Ten disorder groups were treated by the people surveyed. These were mainly digestive disorders (D) and reproductive disorders (W), both presenting a ICF value of 0.8. A total of 101 medicinal plants belonging to 42 families and 90 genera were recorded. Spondias mombin, Zanthoxylum zanthoxyloides and Khaya senegalensis were the most important plants with CI values of 0.208, 0.125 and 0.121, respectively. Gender, ethnicity, agro-ecological zone and flocks size were the socioeconomic and environmental factors that significantly influenced the level of ethnoveterinary knowledge. Chemical and biological analysis are needed on less studied plants such as, Striga hermonthica, Crossopteryx febrifuga, Elaeis guineensis and Momordica charantia.