Programmed cell death 5 factor enhances triptolide-induced fibroblast-like synoviocyte apoptosis of rheumatoid arthritis.

OBJECTIVE: To study the effect of programmed cell death 5 (PDCD5) on apoptosis of rheumatoid arthritis fibroblast-like synoviocyte (RAFLS) induced by triptolide. METHOD: Cultured synovial cells in vitro from RA patients were transfected with Ad-PDCD5. In protein level, expression of PDCD5 protein in Ad-PDCD5 transfected RAFLS was detected by Western blot. RAFLS transfected with Ad-PDCD5 were cultured in presence or absence of triptolide and RAFLs apoptosis was determined by flow cytometry. RESULT: Transfection of RAFLS with increasing concentration of Ad-PDCD5 (50-300 MOI) resulted in dose-dependent increase of PDCD5 production. Apoptotic cells percentage of no transfection group, Ad-null group and Ad-PDCD5 group were, respectively, (22.41 +/- 3.87)%, (28.77 +/- 12.97)%, and (48.87 +/- 12.69)%. Alternatively, transfection without triptolide stimuli had no effect. The data showed that gene transfection of PDCD5 alone without triptolide was not sufficient to activate RAFLS apoptosis; PDCD5 acted as an enhancer rather than inductor of apoptosis. CONCLUSION: Overexpression of PDCD5 could enhance apoptosis of RA FLS induced by triptolide; PDCD5 may be a potential therapeutic target to RA.

Revision for infected total hip arthroplasty / 中华创伤骨科杂志

Objective To discuss operative treatment of the postoperative infection after total hip arthroplasty. Methods 11 cases of infected hip arthroplasty, 6 males and 5 females, with a mean age of 63 years old (from 54 to 71 years), were treated operatively in our department. Their preoperative mean Harris score was 33 (from 25 to 40), and their mean erythrocyte sedimentation rate (ESR) was 61 mm/h (from 34 to 80 mm/h). Bacterial cultures and drug susceptibility tests were carried out before and during operation. One- stage revision was performed for 6 cases, and two- stage revision was performed for 5 cases. Of the 11 cases, 2 were classified as early postoperative infection and 9 as late chronic infection. Preoperative microorganism cultures of joint aspiration or pus in sinus were positive in 8 cases but negative in 3 cases. 5 had staphylococcus epidermidis infection, 2 had escherichia coli infection and 1 had staphylococcus aureus infection. Results No recurrence of infection was found after a mean follow- up period of 17 months (from 12 to 31 months). ESR and blood tests after operation revealed nothing abnormal. The mean Harris score increased significantly to 84.2 (from 79 to 92) after revision (P

Glove reinforcement: an alternative to double gloving.

Gloves, worn by the surgical team to prevent transmission of infections from and to patients, are prone to tears and perforations. This study was done to determine the frequency and sites of unrecognized glove perforation during surgical procedures. The percentage of glove perforation was 14%. Of the punctures, 73% occurred in one of four contiguous locations on the glove. We recommend glove reinforcement at these locations to provide better protection, as well as to reduce the burden of double gloving.

Psoriasiform dermatosis in HLA-B2704 transgenic mice / 中华风湿病学杂志

Objective To confirm the role of HLA B2704 in the pathogenesis of psoriasiform dermatosis by investigating the phenotype of skin diseases in HLA B2704 transgenic mice.Methods The transgenic positive mice were screened and identified by PCR,dot blot and Southern blot hybridization.HE and Immunohistochemistry staining of the diseased mice skin were performed to detect the pathological changes and expression of HLA B2704.Results Six transgenic positive mice developed obvious psoriasiform dermatosis.Histologically,the skin was massively thickened by psoriasiform hyperplasia and keratinization.HLA B2704 antigen was highly expressed on the cell surface.Conclusion HLA B2704 heavy chain can induce the psoriasiform dermatosis in the transgenic mice.

Experimental study of nerve graft with vascular implantation.

OBJECTIVE: To study the performance of free nerve graft with vascular implantation. MATERIAL AND METHOD: Three groups of rats were used. A 10 mm nerve defect was made in the sciatic nerve. It was repaired respectively with vascularised nerve graft (VNG), free nerve graft with vascular implantation (NGV) or free nerve graft (NG). Before 30 days the revascularisation of the nerve graft was assessed; at 2 to 3 month regeneration of nerve was evaluated. Histochemical staining, vascular morphology and electrophysiological examination were undertaken. RESULT AND CONCLUSION: The number of regenerated blood vessels in VNG and NGV was significantly increased than that in NG before 30 days. The ratio of regenerated nerve fiber area and conduction velocity in the first two groups were much better than those in NG at 2 to 3 months.

Effect of soft tissue balance in total knee replacement of varus knee / 中华骨科杂志

Objective To analyze contribution of varus knee with osteoarthritis and investigate the role of soft tissue balance in total knee replacement ( TKR) of that. Methods One hundred cases (145 varus knees) were operated with TKR. There were 18 males (25 knees) and 82 females (120 knees), with the average age of 64.6 years, from 45 to 80 years old. During the operation, accurate osteotomy and soft tissue balance was made to correct the varus knee joint. The contribution angles of varus knee was measured on weight bearing X ray images pre and postoperatively, in order to evaluate the results of the combination of the osteotomy and soft tissue balance. Results The gross varus angle was average 9.2? ? 3.1? , ranging from 5? to 30? preoperatively. In the total angle, the varus angle caused by soft tissue imbalance was 53.2% , the osseous varus angles caused by the tibia varus was 22.8% , the osseous varus angles caused by tibia plateau destruction was 24.0% . The measurement of the postoperative X ray films suggested that the average angle of the tibia plateau osteotomy was 4.3? , obtaining the correction of 27.9% of the total varus angle, and the angles corrected by soft tissue balance was 10.7? ,getting the correction of 72.1% of the total varus angle. According to HSS score system, the preoperative score was 38 points, ranging from 5 to 57, and postoperative scores was 87 points, ranging from 75 to 90. Conclusion The contribution of varus knee with osteoarthritis were include: 1) Bony structural varus angle composed of tibial varus and tibial plateau destruction; 2) Collateral ligaments and soft tissue imbalance around the knee. Articular surface destruction and soft tissue imbalance are the main contribution of varus knee. For TKR of varus knees like this, besides accurate osteotomy, correct soft tissue balance is the key of the operation. [