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1.
PLoS Pathog ; 19(1): e1011003, 2023 01.
Article in English | MEDLINE | ID: mdl-36603017

ABSTRACT

Toxoplasma gondii (T. gondii) is a zoonotic apicomplexan parasite that is an important cause of clinical disability in humans. On a global scale, one third of the human population is infected with T. gondii. Mice and other small rodents are believed to be responsible for transmission of T. gondii to the domestic cat, its definitive host. Interferon-inducible Immunity-Related GTPases (IRG proteins) are important for control of murine T. gondii infections. Virulence differences between T. gondii strains are linked to polymorphic rhoptry proteins (ROPs) that cooperate to inactivate individual IRG family members. In particular, the pseudokinase ROP5 isoform B is critically important in laboratory strains of mice. We identified T. gondii ROP39 in complex with ROP5B and demonstrate its contribution to acute T. gondii virulence. ROP39 directly targets Irgb10 and inhibits homodimer formation of the GTPase leading to an overall reduction of IRG protein loading onto the parasitophorous vacuolar membrane (PVM). Maintenance of PVM integrity rescues the parasite from IRG protein-mediated clearance in vitro and in vivo. This study identifies a novel T. gondii effector that is important for specific inactivation of the IRG resistance system. Our data reveal that yet unknown T. gondii effectors can emerge from identification of direct interaction partners of ROP5B.


Subject(s)
Parasites , Toxoplasma , Toxoplasmosis , Animals , Mice , Humans , Cats , Toxoplasma/metabolism , Parasites/metabolism , Virulence , Protozoan Proteins/genetics , Protozoan Proteins/metabolism , GTP Phosphohydrolases/metabolism
2.
Mem Inst Oswaldo Cruz ; 104(2): 234-40, 2009 Mar.
Article in English | MEDLINE | ID: mdl-19430648

ABSTRACT

The Immunity Related GTPases (IRG proteins) constitute a large family of interferon-inducible proteins that mediate early resistance to Toxoplasma gondii infection in mice. At least six members of this family are required for resistance of mice to virulent T. gondii strains. Recent results have shown that the complexity of the resistance arises from complex regulatory interactions between different family members. The mode of action against T. gondii depends on the ability of IRG proteins to accumulate on the parasitophorous vacuole of invading tachyzoites and to induce local damage to the vacuole resulting in disruption of the vacuolar membrane. Virulent strains of T. gondii overcome the IRG resistance system, probably by interfering with the loading of IRG proteins onto the parasitophorous vacuole membrane. It may be assumed that T. gondii strains highly virulent for mice will be disadvantaged in the wild due to the rapid extinction of the infected host, while it is self-evident that susceptibility to virulent strains is disadvantageous to the mouse host. We consider the possibility that this double disadvantage is compensated in wild populations by segregating alleles with different resistance and susceptibility properties in the IRG system.


Subject(s)
GTP Phosphohydrolases/immunology , Immunity, Innate/immunology , Toxoplasma/pathogenicity , Toxoplasmosis, Animal/immunology , Animals , GTP Phosphohydrolases/metabolism , Host-Parasite Interactions/immunology , Mice , Toxoplasma/immunology , Toxoplasmosis, Animal/enzymology
3.
Mem. Inst. Oswaldo Cruz ; 104(2): 234-240, Mar. 2009. ilus
Article in English | LILACS | ID: lil-533512

ABSTRACT

The Immunity Related GTPases (IRG proteins) constitute a large family of interferon-inducible proteins that mediate early resistance to Toxoplasma gondii infection in mice. At least six members of this family are required for resistance of mice to virulent T. gondii strains. Recent results have shown that the complexity of the resistance arises from complex regulatory interactions between different family members. The mode of action against T. gondii depends on the ability of IRG proteins to accumulate on the parasitophorous vacuole of invading tachyzoites and to induce local damage to the vacuole resulting in disruption of the vacuolar membrane. Virulent strains of T. gondiiovercome the IRG resistance system, probably by interfering with the loading of IRG proteins onto the parasitophorous vacuole membrane. It may be assumed that T. gondii strains highly virulent for mice will be disadvantaged in the wild due to the rapid extinction of the infected host, while it is self-evident that susceptibility to virulent strains is disadvantageous to the mouse host. We consider the possibility that this double disadvantage is compensated in wild populations by segregating alleles with different resistance and susceptibility properties in the IRG system.


Subject(s)
Animals , Mice , GTP Phosphohydrolases/immunology , Immunity, Innate/immunology , Toxoplasma/pathogenicity , Toxoplasmosis, Animal/immunology , GTP Phosphohydrolases/metabolism , Host-Parasite Interactions/immunology , Toxoplasma/immunology , Toxoplasmosis, Animal/enzymology
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