ABSTRACT
Atlantic salmon Salmo salar microsatellite markers from a large database were analysed and selected with technical, economic and genetic criteria to provide an optimized set of polymorphic DNA markers for the analysis of the genetic diversity and the structure of anadromous Atlantic salmon populations. A set of 37 microsatellite markers was identified that are easy to use and provide a high level of differentiation power.
Subject(s)
Genetic Variation , Genetics, Population , Microsatellite Repeats , Salmo salar/genetics , Animals , Sequence Analysis, DNAABSTRACT
The origins, design, fabrication and performance of an Atlantic salmon microarray are described. The microarray comprises 16 950 Atlantic salmon-derived cDNA features, printed in duplicate and mostly sourced from pre-existing expressed sequence tag (EST) collections [SALGENE and salmon genome project (SGP)] but also supplemented with cDNAs from suppression subtractive hybridization libraries and candidate genes involved in immune response, protein catabolism, lipid metabolism and the parr-smolt transformation. A preliminary analysis of a dietary lipid experiment identified a number of genes known to be involved in lipid metabolism. Significant fold change differences (as low as 1.2x) were apparent from the microarray analysis and were confirmed by quantitative real-time polymerase chain reaction analysis. The study also highlighted the potential for obtaining artefactual expression patterns as a result of cross-hybridization of similar transcripts. Examination of the robustness and sensitivity of the experimental design employed demonstrated the greater importance of biological replication over technical (dye flip) replication for identification of a limited number of key genes in the studied system. The TRAITS (TRanscriptome Analysis of Important Traits of Salmon)-salmon genome project microarray has been proven, in a number of studies, to be a powerful tool for the study of key traits of Atlantic salmon biology. It is now available for use by researchers in the wider scientific community.
ABSTRACT
Interferons (IFNs) are cytokines that have proinflammatory, antiviral, and immunomodulatory effects and play a central role during a host response to pathogens. The IFN family contains both type I and type II molecules. While there are a number of type I IFNs, there is only one type II IFN. Recently both type I and type II IFN genes have been cloned in salmonid fish and recombinant proteins produced showing IFN activity. We have stimulated an Atlantic salmon cell line (SHK-1) with both type I and type II recombinant salmonid IFNs and analyzed the transcriptional response by microarray analysis. Cells were exposed to recombinant IFNs for 6 or 24 h or left unexposed as controls. RNA was hybridized to an Atlantic salmon cDNA microarray (salmon 17K feature TRAITS/SGP array) in order to assess differential gene expression in response to IFN exposure. For IFN I and II, 47 and 72 genes were stimulated, respectively; most genes were stimulated by a single IFN type, but some were affected by both IFNs, indicating coregulation of the IFN response in fish. Real-time PCR analysis was employed to confirm the microarray results for selected differentially expressed genes in both a cell line and primary leukocyte cultures.
Subject(s)
Interferon Type I/genetics , Interferon-gamma/genetics , Oligonucleotide Array Sequence Analysis , Animals , Cell Culture Techniques/methods , Cell Line , Cloning, Molecular , Cytokines/genetics , DNA, Complementary/genetics , Recombinant Proteins/metabolism , Salmo salar/physiologyABSTRACT
We have integrated data from linkage mapping, physical mapping and karyotyping to gain a better understanding of the sex-determining locus, SEX, in Atlantic salmon (Salmo salar). SEX has been mapped to Atlantic salmon linkage group 1 (ASL1) and is associated with several microsatellite markers. We have used probes designed from the flanking regions of these sex-linked microsatellite markers to screen a bacterial artificial chromosome (BAC) library, representing an 11.7x coverage of the Atlantic salmon genome, which has been HindIII fingerprinted and assembled into contigs. BACs containing sex-linked microsatellites and their related contigs have been identified and representative BACs have been placed on the Atlantic salmon chromosomes by fluorescent in situ hybridization (FISH). This identified chromosome 2, a large metacentric, as the sex chromosome. By positioning several BACs on this chromosome by FISH, it was possible to orient ASL1 with respect to chromosome 2. The region containing SEX appears to lie on the long arm between marker Ssa202DU and a region of heterochromatin identified by DAPI staining. BAC end-sequencing of clones within sex-linked contigs revealed five hitherto unmapped genes along the sex chromosome. We are using an in silico approach coupled with physical probing of the BAC library to extend the BAC contigs to provide a physical map of ASL1, with a view to sequencing chromosome 2 and, in the process, identifying the sex-determining gene.
Subject(s)
Chromosome Mapping , Salmo salar/genetics , Animals , Base Sequence , Chromosomes, Artificial, Bacterial , DNA Primers , Female , In Situ Hybridization, Fluorescence , Male , Microsatellite Repeats , Proteins/genetics , Sex Determination ProcessesABSTRACT
Plectin is one of the largest and most versatile cytolinker proteins known. Cloned and sequenced in 1991, it was later shown to have nonsense mutations in recessive epidermolysis bullosa with muscular dystrophy. A dominant mutation in the gene was found to cause epidermolysis bullosa simplex Ogna without muscular dystrophy. Here we report the DNA sequencing of the plectin gene (PLEC1) in a Dutch family originally described in 1972 as having epidermolysis bullosa with muscular dystrophy. The results revealed homozygosity for a new plectin nonsense mutation at position 13187 and its specific 8q24 marker haplotype profile. Western blotting of cultured fibroblasts and immunofluorescence microscopy of skin biopsy confirm that the plectin protein expression is grossly reduced or absent. A summary of the life-long clinical course of the two affected brothers homozygous for the new E1914X mutation is given.
Subject(s)
Epidermolysis Bullosa Simplex/genetics , Genes, Dominant , Genetic Predisposition to Disease , Intermediate Filament Proteins/genetics , Muscular Dystrophies/genetics , Point Mutation , Base Sequence , Blotting, Western , Chronic Disease , Epidermolysis Bullosa Simplex/complications , Epidermolysis Bullosa Simplex/pathology , Fluorescent Antibody Technique , Genetic Linkage , Humans , Male , Molecular Sequence Data , Muscular Dystrophies/complications , Muscular Dystrophies/pathology , Norway , Pedigree , Phenotype , Plectin , Reverse Transcriptase Polymerase Chain Reaction , Sampling Studies , Severity of Illness Index , SiblingsABSTRACT
We constructed a genetic linkage map for Arctic char (Salvelinus alpinus) using two backcrosses between genetically divergent strains. Forty-six linkage groups (expected = 39-41) and 19 homeologous affinities (expected = 25) were identified using 184 microsatellites, 129 amplified fragment length polymorphisms (AFLPs), 13 type I gene markers, and one phenotypic marker, SEX. Twenty-six markers remain unlinked. Female map distance (9.92 Morgans) was substantially higher than male map distance (3.90 Morgans) based on the most complete parental information (i.e., the F1 hybrids). Female recombination rates were often significantly higher than those of males across all pairwise comparisons within homologous chromosomal segments (average female to male ratios within families was 1.69:1). The female hybrid parent had significantly higher recombination rates than the pure strain female parent. Segregation distortion was detected in four linkage groups (4, 8, 13, 20) for both families. In family 3, only the largest fish were sampled for genotyping, suggesting that segregation distortion may represent regions possessing influences on growth. In family 2, almost all cases showing segregation distortion involved markers in the female hybrid parent.
Subject(s)
Genetic Linkage , Recombination, Genetic , Trout/genetics , Animals , Chimera/genetics , Chromosome Mapping , Crosses, Genetic , Female , Genetic Markers , Inbreeding , Male , Microsatellite Repeats , Polymerase Chain Reaction , Polymorphism, Single NucleotideABSTRACT
A genetic linkage map of the Atlantic salmon (Salmo salar) was constructed, using 54 microsatellites and 473 amplified fragment length polymorphism (AFLP) markers. The mapping population consisted of two full-sib families within one paternal half-sib family from the Norwegian breeding population. A mapping strategy was developed that facilitated the construction of separate male and female maps, while retaining all the information contributed by the dominant AFLP markers. By using this strategy, we were able to map a significant number of the AFLP markers for which all informative offspring had two heterozygous parents; these markers then served as bridges between the male and female maps. The female map spanned 901 cM and had 33 linkage groups, while the male spanned 103 cM and had 31 linkage groups. Twenty-five linkage groups were common between the two maps. The construction of the genetic map revealed a large difference in recombination rate between females and males. The ratio of female recombination rate vs. male recombination rate was 8.26, the highest ratio reported for any vertebrate. This map constitutes the first linkage map of Atlantic salmon, one of the most important aquaculture species worldwide.
Subject(s)
Chromosome Mapping , Recombination, Genetic/genetics , Salmo salar/genetics , Animals , DNA Primers , Female , Male , Microsatellite Repeats/genetics , Norway , Polymorphism, Restriction Fragment Length , Sex FactorsSubject(s)
Aspartic Acid/genetics , Genetic Testing , Melanoma/genetics , Receptors, Pituitary Hormone/genetics , Skin Neoplasms/genetics , Base Sequence , Female , Gene Frequency , Humans , Male , Molecular Sequence Data , Norway , Point Mutation , Polymerase Chain Reaction , Prospective Studies , Sensitivity and Specificity , alpha-MSH/geneticsABSTRACT
We constructed a genetic linkage map for a tetraploid derivative species, the rainbow trout (Oncorhynchus mykiss), using 191 microsatellite, 3 RAPD, 7 ESMP, and 7 allozyme markers in three backcross families. The linkage map consists of 29 linkage groups with potential arm displacements in the female map due to male-specific pseudolinkage arrangements. Synteny of duplicated microsatellite markers was used to identify and confirm some previously reported pseudolinkage arrangements based upon allozyme markers. Fifteen centromeric regions (20 chromosome arms) were identified with a half-tetrad analysis using gynogenetic diploids. Female map length is approximately 10 M, but this is a large underestimate as many genotyped segments remain unassigned at a LOD threshold of 3.0. Extreme differences in female:male map distances were observed (ratio F:M, 3.25:1). Females had much lower recombination rates (0.14:1) in telomeric regions than males, while recombination rates were much higher in females within regions proximal to the centromere (F:M, 10:1). Quadrivalent formations that appear almost exclusively in males are postulated to account for the observed differences.
Subject(s)
Chromosome Mapping , Microsatellite Repeats/genetics , Oncorhynchus mykiss/genetics , Recombination, Genetic , Animals , Chromosomes/genetics , Female , Genetic Markers , Inbreeding , Lod Score , Male , Polymorphism, Restriction Fragment Length , Random Amplified Polymorphic DNA Technique , Sex Determination Processes , Sex FactorsABSTRACT
The porcine hormone-sensitive lipase gene and its cDNA have been isolated and sequenced. Several putative regulatory sequences have been detected in the promotor region. The deduced amino acid sequence is 85% identical to the corresponding human, mouse and rat sequence. A search for polymorphisms revealed one intronic and one exonic polymorphism, the latter resulting in a conservative amino acid substitution. Linkage mapping located the LIPE gene close to the calcium release channel (CRC) locus on chromosome 6.
Subject(s)
Chromosome Mapping/veterinary , Polymorphism, Genetic , Sterol Esterase/genetics , Amino Acid Sequence , Animals , DNA, Complementary/chemistry , DNA, Complementary/isolation & purification , Gene Expression Regulation, Enzymologic , Gene Library , Genetic Variation , Humans , Introns , Mice , Molecular Sequence Data , Rats , Restriction Mapping/veterinary , Sequence Alignment/veterinary , SwineABSTRACT
A quantitative trait locus (QTL) analysis of growth and fatness data from a three generation pig experiment is presented. The population of 199 F2 animals was derived from a cross between wild boar and Large White pigs. Animals were typed for 240 markers spanning 23 Morgans of 18 autosomes and the X chromosome. A series of analyses are presented within a least squares framework. First, these identify chromosomes containing loci controlling trait variation and subsequently attempt to map QTLs to locations within chromosomes. This population gives evidence for a large QTL affecting back fat and another for abdominal fat segregating on chromosome 4. The best locations for these QTLs are within 4 cM of each other and, hence, this is likely to be a single QTL affecting both traits. The allele inherited from the wild boar causes an increase in fat deposition. A QTL for intestinal length was also located in the same region on chromosome 4 and could be the same QTL with pleiotropic effects. Significant effects, owing to multiple QTLs, for intestinal length were identified on chromosomes 3 and 5. A single QTL affecting growth rate to 30 kg was located on chromosome 13 such that the Large White allele increased early growth rate, another QTL on chromosome 10 affected growth rate from 30 to 70 kg and another on chromosome 4 affected growth rate to 70 kg.
Subject(s)
Chromosome Mapping , Crosses, Genetic , Quantitative Trait, Heritable , Swine/genetics , Animals , Chromosome Mapping/statistics & numerical data , Confidence Intervals , Female , Genetic Carrier Screening , Genetic Linkage , Genetic Markers , Genetic Variation , Genomic Imprinting , Halothane , Least-Squares Analysis , Male , Models, GeneticABSTRACT
Comparative mapping data suggested that the dominant white coat color in pigs may be due to a mutation in KIT which encodes the mast/stem cell growth factor receptor. We report here that dominant white pigs lack melanocytes in the skin, as would be anticipated for a KIT mutation. We found a complete association between the dominant white mutation and a duplication of the KIT gene, or part of it, in samples of unrelated pigs representing six different breeds. The duplication was revealed by single strand conformation polymorphism (SSCP) analysis and subsequent sequence analysis showing that white pigs transmitted two nonallelic KIT sequences. Quantitative Southern blot and quantitative PCR analysis, as well as fluorescence in situ hybridization (FISH) analysis, confirmed the presence of a gene duplication in white pigs. FISH analyses showed that KIT and the very closely linked gene encoding the platelet-derived growth factor receptor (PDGFRA) are both located on the short arm of Chromosome (Chr) 8 at band 8p12. The result revealed an extremely low rate of recombination in the centromeric region of this chromosome, since the closely linked (0.5 cM) serum albumin (ALB) locus has previously been in situ mapped to the long arm (8q12). Pig Chr 8 shares extensive conserved synteny with human Chr 4, but the gene order is rearranged.
Subject(s)
Chromosome Mapping , Chromosomes, Human, Pair 4 , Hair Color/genetics , Proto-Oncogene Proteins c-kit/genetics , Swine/genetics , Animals , Base Sequence , DNA Primers , Exons , Genes, Dominant , Genetic Linkage , Genetic Markers , Humans , In Situ Hybridization, Fluorescence , Molecular Sequence Data , Phenotype , Polymerase Chain Reaction , Polymorphism, Single-Stranded Conformational , Recombination, Genetic , Sequence Homology, Nucleic AcidABSTRACT
A comprehensive linkage map, including 236 linked markers with a total sex-average map length of about 2300 cM, covering nearly all parts of the pig genome has been established. Linkage groups were assigned to approximately all 18 autosomes, the X chromosome and the X/Y pseudoautosomal region. Several new gene assignments were made including the assignment of linkage group U1 (EAK-HPX) to chromosome 9. The linkage map includes 77 types I loci informative for comparative mapping and 72 in situ mapped markers physically anchoring the linkage groups on chromosomes. A highly significant heterogeneity in recombination rates between sexes was observed with a general tendency towards an excess of female recombination. The average ratio of female to male recombination was estimated at 1.4:1 but this parameter varied between chromosomes as well as between regions within chromosomes. An intriguing finding was that blood group loci were overrepresented at the distal ends of linkage groups.
Subject(s)
Chromosome Mapping , Swine/genetics , Animals , Crosses, Genetic , Enzymes/genetics , Female , Genetic Markers , Male , Polymorphism, Restriction Fragment Length , Proteins/genetics , Recombination, Genetic , Sex CharacteristicsABSTRACT
A linkage map of pig chromosome 6 was constructed using a wild pig/Large White intercross pedigree. The map comprises 23 polymorphic loci, and the sex-average map length is approximately 170 cM. The study adds three new genes to the chromosome 6 map: the extension (E) coat color locus, and the blood group O (EAO) and tyrosine aminotransferase (TAT) loci. Segregation at the E locus determined two coat color phenotypes among the F2 animals: wild-type color (El-) and black-spotting (Ep/Ep). The E locus showed close genetic linkage to the most distal marker (S0035) on the short arm of chromosome 6. Comparative coat color genetics as well as comparative mapping strongly suggest that E in pigs encodes the melanocyte-stimulating hormone receptor, as previously shown for the corresponding coat color loci in mouse and cattle. TAT was also mapped to the distal part of 6p, whereas EAO was the most distal marker on 6q. A clear tendency for a higher recombination rate in both terminal regions was observed. A model for the evolution of pig chromosome 6, based on comparative mapping data, is presented.
Subject(s)
ABO Blood-Group System/genetics , Chromosome Mapping/veterinary , Hair Color/genetics , Swine/genetics , Tyrosine Transaminase/genetics , Animals , Cattle , Female , Genetic Linkage , Humans , Male , Mice , Phenotype , Polymorphism, Restriction Fragment Length , Swine/bloodABSTRACT
The chromosomes of the babirusa, a species considered to have diverged from an ancestor of the pig during the Miocene epoch, about 12-26 million years ago, were studied to determine the sites of recent rearrangements during evolution of the domestic pig. It is shown that there is a pericentric inversion of the entire short arm on pig chromosome 1, compared to its counterpart in the babirusa (chromosome 15). We also present evidence suggesting that pig chromosome 3 was derived by a telomere-centromere fusion of two ancestral chromosomes homoelogous to babirusa chromosomes 12 and 17. Likewise, we conclude that pig chromosome 6 was most likely derived by a telomere-telomere fusion of ancestral chromosomes homoelogous to babirusa chromosomes 6 and 14. The detection of interstitial hybridization signals from presumptive subteloemeric repeats in the same chromosome region as the evolutionary fusion points on pig chromosomes 3 and 6 indicates that the fusion sites may still contain elements that are otherwise restricted to the telomere regions of pig chromosomes.