In situ monitoring of selective laser melting using plume and spatter signatures by deep belief networks.

Critical quality issues such as high porosity, cracks, and delamination are common in current selective laser melting (SLM) manufactured components. This study provides a flexible and integrated method for in situ process monitoring and melted state recognition during the SLM process, and it is useful for process optimization to decrease part quality issues. The part qualities are captured by images obtained from an off-axis setup with a near-infrared (NIR) camera. Plume and spatter signatures are closely related to the melted states and laser energy density, and they are employed for the SLM process monitoring in an adapted deep belief network (DBN) framework. The melted state recognition with the improved DBN and original NIR images requires little signal preprocessing, less parameter selection and feature extraction, obtaining the classification rate 83.40% for five melted states. Compared to the other methods of neural network (NN) and convolutional neural networks (CNN), the proposed DBN approach is identified to be accurate, convenient, and suitable for the SLM process monitoring and part quality recognition.

Triply Periodic Minimal Surfaces Sheet Scaffolds for Tissue Engineering Applications: An Optimization Approach toward Biomimetic Scaffold Design.

Biomimetic scaffold design is gaining attention in the field of tissue engineering lately. Recently, triply periodic minimal surfaces (TPMSs) have attracted the attention of tissue engineering scientists for fabrication of biomimetic porous scaffolds. TPMS scaffolds offer several advantages, which include a high surface area to volume ratio, less stress concentration, and increased permeability compared to the traditional lattice structures, thereby aiding in better cell adhesion, migration, and proliferation. In literature, several design methods for TPMS scaffolds have been developed, which considered some of the important tissue-specific requirements, such as porosity, Young's modulus, and pore size. However, only one of the requirements of a tissue engineering scaffold was investigated in these studies, and not all of the requirements were satisfied simultaneously. In this work, we develop a design method for TPMS sheet scaffolds, which is able to satisfy multiple requirements including the porosity, Young's modulus, and pore size, based on a parametric optimization approach. Three TPMSs, namely, the primitive (P), gyroid (G), and diamond (D) surfaces, with cubic symmetry are considered. The versatility of the proposed design method is demonstrated by three different applications, namely, tissue-specific scaffolds, scaffolds for stem cell differentiation, and functionally graded scaffolds with biomimetic functional gradients.

Collagen grafted 3D polycaprolactone scaffolds for enhanced cartilage regeneration.

Current surgical and repair treatments for articular cartilage defects still do not give satisfactory long-term results. Scaffold-based tissue engineering is the subject of much intensive interest. However, one major hurdle is that it is unable to accurately replicate the internal three dimensional (3D) microstructure of cartilage. In this work, a novel electrohydrodynamic printing (E-Jetting) technique was employed to fabricate 3D polycaprolactone (PCL) scaffolds, followed by collagen grafting mediated by polydopamine. Surface topography, chemical composition, and wettability of the scaffolds before and after surface functionalization were characterized. Porcine chondrocytes were seeded within the scaffolds for chondrogenesis evaluation. The results showed that a 3D PCL scaffold with controlled fibre diameter, orientation, and pore size was fabricated by the E-Jetting system. The surface functionalization made the PCL scaffold hydrophilic and favourable for cell attachment. The chondrocytes maintained their healthy phenotypes within the collagen grafted PCL scaffold. The increased production of sulfated glycosaminoglycan and highly expressed collagen type II demonstrated that collagen had a positive role in stimulating chondrogenesis and the collagen grafted PCL scaffold was effective in cartilage regeneration.