ABSTRACT
The human promyelocytic cell line HL-60 can be induced to monocytoid terminal differentiation by several conditioned media produced by lectin-stimulated mononuclear cells. We reported previously that a 572-conditioned medium (CM) secreted from viscum alniformosanae-stimulated mononuclear cells also had the capacity of inducing HL-60 leukemic cells into mature monocytes. In the present study, we showed that 572-CM did not contain IFN-r, TNF, IL-1 and IL-2 as determined by using ELISA tests. This CM was unable to induce granulocyte-macrophage colony formation. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was used to detect the components of this CM. After running the acrylamide gels, a wide band protein, in the 65-80 kd range was obtained and it was different from those of other mitogens.
Subject(s)
Cell Differentiation/drug effects , Culture Media, Conditioned/pharmacology , Drugs, Chinese Herbal/pharmacology , Tumor Cells, Cultured/drug effects , Culture Media, Conditioned/chemistry , Drugs, Chinese Herbal/chemistry , Electrophoresis, Polyacrylamide Gel , Enzyme-Linked Immunosorbent Assay , Evaluation Studies as Topic , Granulocyte-Macrophage Colony-Stimulating Factor/chemistry , Humans , Interferons/chemistry , Interleukin-1/chemistry , Interleukin-2/chemistry , Leukemia, Promyelocytic, Acute , Monocytes , Tumor Necrosis Factor-alpha/chemistryABSTRACT
A 28-year-old female patient with acute myelogenous leukemia and post-transfusion chronic viral hepatitis C received an allogeneic bone marrow transplantation (BMT) in first complete remission. The inflammatory activity of chronic hepatitis C decreased dramatically with the start of BMT and during the whole course of cyclosporin A treatment. After the immunosuppressive therapy was stopped, however, the chronic hepatitis C activity increased and the serum alanine amino-transferase levels thereafter remained as high as before BMT. Hepatitis C virus RNA was detected by polymerase chain reaction in serum drawn 60 days and 52 months after BMT. Since fulminant viral hepatitis B may take place after tapering of immunosuppressive therapy following BMT due to an excessive response of recovering immunity to the actively replicating virus during immune suppression, careful monitoring of liver function for patients with chronic viral hepatitis C in BMT is suggested.
Subject(s)
Bone Marrow Transplantation/adverse effects , Cyclosporine/adverse effects , Hepatitis C/etiology , Hepatitis, Chronic/etiology , Adult , Base Sequence , Cyclosporine/administration & dosage , DNA, Viral/genetics , DNA, Viral/isolation & purification , Female , Hepacivirus/genetics , Hepacivirus/isolation & purification , Humans , Leukemia, Myeloid, Acute/surgery , Molecular Sequence DataABSTRACT
A conditioned medium(CM), designated as 572-CMF-, was a Chinese herb viscum alniformosanae (V.A.) stimulated mononuclear cells. This CM has the capacity to induce the promyelocytic cell line HL-60 to differentiate into morphologically and functionally mature monocytoid cells. However, our results on the effect of a combination of 572 conditioned medium and IFN-r, TNF and IL-2 were neither synergistic nor additive. Further investigation of the nature of this conditioned medium remains to be performed.
Subject(s)
Cell Differentiation/drug effects , Drugs, Chinese Herbal/pharmacology , Leukemia, Experimental/physiopathology , Leukocytes, Mononuclear/drug effects , Mistletoe/physiology , Plants, Medicinal , Cell Line, Transformed/drug effects , Culture Media , Humans , Interferons/physiology , Interleukin-2/physiology , Leukocyte Count , Phagocytosis/drug effects , Tumor Necrosis Factor-alpha/physiologyABSTRACT
The promyelocytic cell line HL-60 could be induced to differentiate into morphologically and functionally mature monocytoid cells (up to 20%) following exposure to the Chinese herb Clerodendron Fragrans (1 mg/ml). This effect was time dependent and appeared to work synergistically with interferon-r in this promotion of differentiation. Our study suggests that Clerodendron Fragrans has potential therapeutic value for the treatment of certain acute myelocytic leukemia putatively caused by a block in the myeloid differentiation process.
