ABSTRACT
RNAs are capable of modulating immune responses by binding to specific receptors. Neutrophils represent the major fraction of circulating immune cells, but receptors and mechanisms by which neutrophils sense RNA are poorly defined. Here, we analyzed the mRNA and protein expression patterns and the subcellular localization of the RNA receptors RIG-I, MDA-5, TLR3, TLR7, and TLR8 in primary neutrophils and immortalized neutrophil-like differentiated HL-60 cells. Our results demonstrate that both neutrophils and differentiated HL-60 cells express RIG-I, MDA-5, and TLR8 at the mRNA and protein levels, whereas TLR3 and TLR7 are not expressed at the protein level. Subcellular fractionation, flow cytometry, confocal laser scanning microscopy, and immuno-transmission electron microscopy provided evidence that, besides the cytoplasm, RIG-I and MDA-5 are stored in secretory vesicles of neutrophils and showed that RIG-I and its ligand, 3p-RNA, co-localize at the cell surface without triggering neutrophil activation. In summary, this study demonstrates that neutrophils express a distinct pattern of RNA recognition receptors in a non-canonical way, which could have essential implications for future RNA-based therapeutics.
Subject(s)
DEAD-box RNA Helicases/metabolism , Gene Expression Regulation/physiology , Neutrophil Activation/physiology , RNA , Receptors, Retinoic Acid/metabolism , Toll-Like Receptors/metabolism , DEAD-box RNA Helicases/genetics , Female , HL-60 Cells , Humans , Interferon-Induced Helicase, IFIH1 , Male , Neutrophils , Receptors, Retinoic Acid/genetics , Secretory Vesicles/genetics , Secretory Vesicles/metabolism , Toll-Like Receptors/geneticsABSTRACT
In this paper we study the depolarized backscatter enhancement phenomenon for electromagnetic wave scattering from rough surfaces. Some new experimental data on light scattering from rough metallic surfaces shows a phenomenon of backscattering enhancement existing in the antispecular direction under some conditions, such as the surface parameters, wave polarization and operating frequency. From a roughly random surface the backscattering enhancement is predicted due to the constructive interference of multiple surfaces scattering. The study is based upon the integral equation method modified to be able to predict the phenomenon of multiple scattering and backscattering enhancement. From the study we found the backscattering enhancement takes place on the specialized surface parameters large compared with the incident wavelength. Further we also conclude that the depolarized multiple scattering makes much contribution along the plane of incidence from random rough surfaces, but depolarized single scattering makes little contributions as our expectation. In comparison of model prediction of total multiple scattering strength with measured data along the specular plane, excellent agreement is obtained.
