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Sci Rep ; 12(1): 9668, 2022 06 11.
Article in English | MEDLINE | ID: mdl-35690597

ABSTRACT

Microscopy by Achromatic X-rays With Emission of Laminar Light (MAXWELL) is a new X-ray/visible technique with attractive characteristics including isotropic resolution in all directions, large-volume imaging and high throughput. An ultrathin, laminar X-ray beam produced by a Wolter type I mirror irradiates the sample stimulating the emission of visible light by scintillating nanoparticles, captured by an optical system. Three-dimensional (3D) images are obtained by scanning the specimen with respect to the laminar beam. We implemented and tested the technique with a high-brightness undulator at SPring-8, demonstrating its validity for a variety of specimens. This work was performed under the Synchrotrons for Neuroscience-an Asia-Pacific Strategic Enterprise (SYNAPSE) collaboration.


Subject(s)
Microscopy , Synchrotrons , Imaging, Three-Dimensional , Light , Microscopy/methods , Tomography, X-Ray Computed/methods , X-Rays
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