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1.
Neuroscience ; 347: 85-102, 2017 04 07.
Article in English | MEDLINE | ID: mdl-28215990

ABSTRACT

Single steps in different directions are often used for postural corrections. However, our knowledge about the neural mechanisms underlying their generation is scarce. This study was aimed to characterize the corrective steps generated in response to disturbances of the basic body configuration caused by forward, backward or outward displacement of the hindlimb, as well as to reveal location in the CNS of the corrective step generating mechanisms. Video recording of the motor response to translation of the supporting surface under the hindlimb along with contact forces and activity of back and limb muscles was performed in freely standing intact and in fixed postmammillary rabbits. In intact rabbits, displacement of the hindlimb in any direction caused a lateral trunk movement toward the contralateral hindlimb, and then a corrective step in the direction opposite to the initial displacement. The time difference between onsets of these two events varied considerably. The EMG pattern in the supporting hindlimb was similar for all directions of corrective steps. It caused the increase in the limb stiffness. EMG pattern in the stepping limb differed in steps with different directions. In postmammillary rabbits the corrective stepping movements, as well as EMG patterns in both stepping and standing hindlimbs were similar to those observed in intact rabbits. This study demonstrates that the corrective trunk and limb movements are generated by separate mechanisms activated by sensory signals from the deviated limb. The neuronal networks generating postural corrective steps reside in the brainstem, cerebellum, and spinal cord.


Subject(s)
Brain/physiology , Postural Balance , Animals , Biomechanical Phenomena , Brain Stem/physiology , Electromyography , Feedback, Sensory , Hindlimb/physiology , Muscle, Skeletal/physiology , Neural Pathways/physiology , Rabbits
4.
Cell Death Dis ; 4: e792, 2013 Sep 05.
Article in English | MEDLINE | ID: mdl-24008736

ABSTRACT

Squamous cell carcinoma (SCC) cells refractory to initial chemotherapy frequently develop disease relapse and distant metastasis. We show here that tumor suppressor WW domain-containing oxidoreductase (WWOX) (also named FOR or WOX1) regulates the susceptibility of SCC to methotrexate (MTX) in vitro and cure of SCC in MTX therapy. MTX increased WWOX expression, accompanied by caspase activation and apoptosis, in MTX-sensitive SCC cell lines and tumor biopsies. Suppression by a dominant-negative or small interfering RNA targeting WWOX blocked MTX-mediated cell death in sensitive SCC-15 cells that highly expressed WWOX. In stark contrast, SCC-9 cells expressed minimum amount of WWOX protein and resisted MTX-induced apoptosis. Transiently overexpressed WWOX sensitized SCC-9 cells to apoptosis by MTX. MTX significantly downregulated autophagy-related Beclin-1, Atg12-Atg5 and LC3-II protein expression and autophagosome formation in the sensitive SCC-15, whereas autophagy remained robust in the resistant SCC-9. Mechanistically, WWOX physically interacted with mammalian target of rapamycin (mTOR), which potentiated MTX-increased phosphorylation of mTOR and its downstream substrate p70 S6 kinase, along with dramatic downregulation of the aforementioned proteins in autophagy, in SCC-15. When WWOX was knocked down in SCC-15, MTX-induced mTOR signaling and autophagy inhibition were blocked. Thus, WWOX renders SCC cells susceptible to MTX-induced apoptosis by dampening autophagy, and the failure in inducing WWOX expression leads to chemotherapeutic drug resistance.


Subject(s)
Apoptosis/drug effects , Autophagy/drug effects , Carcinoma, Squamous Cell/drug therapy , Methotrexate/pharmacology , Methotrexate/therapeutic use , Oxidoreductases/metabolism , Tongue Neoplasms/drug therapy , Tumor Suppressor Proteins/metabolism , Carcinoma, Squamous Cell/metabolism , Carcinoma, Squamous Cell/pathology , Carcinoma, Squamous Cell/ultrastructure , Cell Line, Tumor , Down-Regulation/drug effects , Humans , Models, Biological , Neoplasm Proteins/metabolism , Phagosomes/drug effects , Phagosomes/metabolism , Phagosomes/ultrastructure , Signal Transduction/drug effects , TOR Serine-Threonine Kinases/metabolism , Tongue Neoplasms/metabolism , Tongue Neoplasms/pathology , Tongue Neoplasms/ultrastructure , Up-Regulation/drug effects , WW Domain-Containing Oxidoreductase
5.
Genet Couns ; 23(1): 1-7, 2012.
Article in English | MEDLINE | ID: mdl-22611635

ABSTRACT

We report an 82-year-old girl with premature aging, a karyotype of 46,XX and a de novo c.1824C>T mutation encoding p.G608G in the lamin A gene. The clinical features of accelerated aging and the molecular finding were consistent with the diagnosis of Hutchinson-Gilford progeria syndrome (HGPS). In this presentation, we demonstrate the radiological imaging findings of skeletal, oral and craniofacial phenotypes of abnormalities associated with HGPS. The oral and craniofacial abnormalities caused dental caries, severe malocclusion, and swallowing, feeding and speech problems. Dural calcification, and granulation in the ear drum and external ear canal were additionally observed.


Subject(s)
Craniofacial Abnormalities/diagnostic imaging , Dental Caries/diagnostic imaging , Femur/diagnostic imaging , Hand/diagnostic imaging , Progeria/diagnostic imaging , Child , Craniofacial Abnormalities/genetics , Dental Caries/genetics , Female , Humans , Lamin Type A/genetics , Mutation , Progeria/genetics , Radiography
6.
J Neurophysiol ; 108(1): 300-13, 2012 Jul.
Article in English | MEDLINE | ID: mdl-22514291

ABSTRACT

Quadrupeds maintain the dorsal side up body orientation due to the activity of the postural control system driven by limb mechanoreceptors. Binaural galvanic vestibular stimulation (GVS) causes a lateral body sway toward the anode. Previously, we have shown that this new position is actively stabilized, suggesting that GVS changes a set point in the reflex mechanisms controlling body posture. The aim of the present study was to reveal the underlying neuronal mechanisms. Experiments were performed on decerebrate rabbits. The vertebral column was rigidly fixed, whereas hindlimbs were positioned on a platform. Periodic lateral tilts of the platform caused postural limb reflexes (PLRs): activation of extensors in the loaded and flexing limb and a decrease in extensor activity in the opposite (unloaded and extending) limb. Putative spinal interneurons were recorded in segments L4-L5 during PLRs, with and without GVS. We have found that GVS enhanced PLRs on the cathode side and reduced them on the anode side. This asymmetry in PLRs can account for changes in the stabilized body orientation observed in normal rabbits subjected to continuous GVS. Responses to platform tilts (frequency modulation) were observed in 106 spinal neurons, suggesting that they can contribute to PLR generation. Two neuron groups were active in opposite phases of the tilt cycle of the ipsi-limb: F-neurons in the flexion phase, and E-neurons in the extension phase. Neurons were driven mainly by afferent input from the ipsi-limb. If one supposes that F- and E-neurons contribute, respectively, to excitation and inhibition of extensor motoneurons, one can expect that the pattern of response to GVS in F-neurons will be similar to that in extensor muscles, whereas E-neurons will have an opposite pattern. We have found that ~40% of all modulated neurons meet this condition, suggesting that they contribute to the generation of PLRs and to the GVS-caused changes in PLRs.


Subject(s)
Extremities/physiology , Neurons/physiology , Posture/physiology , Reflex/physiology , Spinal Cord/cytology , Vestibular Nerve/physiology , Action Potentials/physiology , Afferent Pathways/physiology , Animals , Decerebrate State , Electric Stimulation/methods , Functional Laterality/physiology , Neural Inhibition/physiology , Neurons/classification , Rabbits , Spinal Cord/physiology
7.
J Neurophysiol ; 107(11): 3020-6, 2012 Jun.
Article in English | MEDLINE | ID: mdl-22402660

ABSTRACT

In quadrupeds, the dorsal-side-up body orientation during standing is maintained due to a postural system that is driven by feedback signals coming mainly from limb mechanoreceptors. In caudally decerebrated (postmammillary) rabbits, the efficacy of this system is considerably reduced. In this paper, we report that the efficacy of postural control in these animals can be restored with galvanic vestibular stimulation (GVS) applied transcutaneously to the labyrinths. In standing intact rabbits, GVS causes a lateral body sway towards the positive electrode. We used this GVS-caused sway to counteract the lateral body sway resulting from a mechanical perturbation of posture. Experiments were performed on postmammillary rabbits that stood on the tilting platform with their hindlimbs. To make the GVS value dependent on the postural perturbation (i.e., on the lateral body sway caused by tilt of the platform), an artificial feedback loop was formed in the following ways: 1) Information about the body sway was provided by a mechanical sensor; 2) The GVS current was applied when the sway exceeded a threshold value; the polarity of the current was determined by the sway direction. This simple algorithm allowed the "hybrid" postural system to maintain the dorsal-side-up orientation of the hindquarters when the platform was tilted by ± 20°. Thus, an important postural function, i.e., securing lateral stability during standing, can be restored in decerebrate rabbits with the GVS-based artificial feedback. We suggest that such a control system can compensate for the loss of lateral stability of various etiologies, and can be used for restoration of balance control in patients with impaired postural functions.


Subject(s)
Decerebrate State/physiopathology , Feedback, Physiological/physiology , Galvanic Skin Response/physiology , Orientation/physiology , Postural Balance/physiology , Vestibule, Labyrinth/physiology , Animals , Electric Stimulation/methods , Electromyography/methods , Rabbits
8.
J Neurophysiol ; 106(3): 1341-54, 2011 Sep.
Article in English | MEDLINE | ID: mdl-21653706

ABSTRACT

In quadrupeds, spinalization in the thoracic region severely impairs postural control in the hindquarters. The goal of this study was to improve postural functions in chronic spinal rabbits by regular application of different factors: intrathecal injection of the 5-HT(2) agonist (±)-1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane hydrochloride (DOI), epidural electrical spinal cord stimulation (EES), and specific postural training (SPT). The factors were used either alone (SPT group) or in combination (DOI+SPT, EES+SPT, and DOI+EES+SPT groups) or not used (control group). It was found that in none of these groups did normal postural corrective movements in response to lateral tilts of the supporting platform reappear within the month of treatment. In control group, reduced irregular electromyographic (EMG) responses, either correctly or incorrectly phased in relation to tilts, were observed. By contrast, in DOI+SPT and EES+SPT groups, a gradual threefold increase in the proportion of correctly phased EMG responses (compared with control) was observed. The increase was smaller in DOI+EES+SPT and SPT groups. Dissimilarly to these long-term effects, short-term effects of DOI and EES were weak or absent. In addition, gradual development of oscillatory EMG activity in the responses to tilts, characteristic for the control group, was retarded in DOI+SPT, EES+SPT, DOI+EES+SPT, and SPT groups. Thus regular application of the three tested factors and their combinations caused progressive, long-lasting plastic changes in the isolated spinal networks, resulting in the facilitation of spinal postural reflexes and in the retardation of the development of oscillatory EMG activity. The facilitated reflexes, however, were insufficient for normal postural functions.


Subject(s)
Electric Stimulation Therapy , Extremities/physiology , Postural Balance/physiology , Serotonin Receptor Agonists/administration & dosage , Spinal Cord Injuries/physiopathology , Spinal Cord Injuries/therapy , Animals , Electric Stimulation Therapy/methods , Electromyography/methods , Injections, Spinal , Male , Postural Balance/drug effects , Rabbits , Thoracic Vertebrae
9.
Cell Death Dis ; 1: e110, 2010 Dec 23.
Article in English | MEDLINE | ID: mdl-21368882

ABSTRACT

The role of a small transforming growth factor beta (TGF-ß)-induced TIAF1 (TGF-ß1-induced antiapoptotic factor) in the pathogenesis of Alzheimer's disease (AD) was investigated. TIAF1 physically interacts with mothers against DPP homolog 4 (Smad4), and blocks SMAD-dependent promoter activation when overexpressed. Accordingly, knockdown of TIAF1 by small interfering RNA resulted in spontaneous accumulation of Smad proteins in the nucleus and activation of the promoter governed by the SMAD complex. TGF-ß1 and environmental stress (e.g., alterations in pericellular environment) may induce TIAF1 self-aggregation in a type II TGF-ß receptor-independent manner in cells, and Smad4 interrupts the aggregation. Aggregated TIAF1 induces apoptosis in a caspase-dependent manner. By filter retardation assay, TIAF1 aggregates were found in the hippocampi of nondemented humans and AD patients. Total TIAF1-positive samples containing amyloid ß (Aß) aggregates are 17 and 48%, respectively, in the nondemented and AD groups, suggesting that TIAF1 aggregation occurs preceding formation of Aß. To test this hypothesis, in vitro analysis showed that TGF-ß-regulated TIAF1 aggregation leads to dephosphorylation of amyloid precursor protein (APP) at Thr668, followed by degradation and generation of APP intracellular domain (AICD), Aß and amyloid fibrils. Polymerized TIAF1 physically interacts with amyloid fibrils, which would favorably support plaque formation in vivo.


Subject(s)
Alzheimer Disease/metabolism , Amyloid beta-Peptides/metabolism , Apoptosis Regulatory Proteins/metabolism , Nuclear Proteins/metabolism , Plaque, Amyloid/metabolism , Transforming Growth Factor beta/pharmacology , Alzheimer Disease/genetics , Alzheimer Disease/pathology , Amyloid beta-Protein Precursor/genetics , Amyloid beta-Protein Precursor/metabolism , Animals , Apoptosis , Apoptosis Regulatory Proteins/genetics , COS Cells , Chlorocebus aethiops , Hippocampus/metabolism , Humans , Mice , Mice, Transgenic , Nuclear Proteins/genetics , Phosphorylation , Plaque, Amyloid/etiology , Polymerization , Signal Transduction , Smad4 Protein/analysis , Smad4 Protein/metabolism , Smad4 Protein/physiology , Transforming Growth Factor beta/metabolism , Two-Hybrid System Techniques
10.
Neuroscience ; 130(2): 397-407, 2005.
Article in English | MEDLINE | ID: mdl-15664696

ABSTRACT

WW domain-containing oxidoreductase WOX1, also named WWOX or FOR, is a known proapoptotic protein and a candidate tumor suppressor. Stress stimuli activate WOX1 via tyrosine 33 (Tyr33) phosphorylation and translocation to the mitochondria and nuclei in vitro. Here, the potential role of WOX1 in light-induced retinal degeneration in vivo was investigated. WOX1 is expressed primarily in the inner retina at perinatal stages, whereas an enhanced expression of WOX1, along with its Tyr33 phosphorylation (p-WOX1), is shown specifically in the retinal ganglion cells in adults. Prolonged exposure of mature rats to constant, low-intensity light (500 lux) for 1-2 months resulted in substantial death of photoreceptors and the presence of activated microglia, astrocytes and Muller glial in the outer retina. However, the inner retina was not or barely affected. In the damaged inner and outer nuclear layers of rat retina, WOX1 and p-WOX1 were overly expressed. Also, WOX1 colocalized with fragments of opsin-positive cones. In rd mice with an inherited retinal deficiency, upregulation of WOX1 and p-WOX1 in degenerated retina was observed with age. By electron microscopy, a large number of immunogold particles of WOX1 and p-WOX1 were found in the damaged mitochondria and condensed nuclei of degenerating photoreceptors, indicating that WOX1 undergoes activation and translocation to these organelles. In contrast, little or no WOX1-positive particles were found in the Golgi apparatus. In conclusion, activated WOX1 is likely to exert apoptosis of neuronal cells in the outer retina during the light-induced injury and in mice with an inherited retinal defect.


Subject(s)
Apoptosis/radiation effects , Light/adverse effects , Neurons/radiation effects , Oxidoreductases/metabolism , Retina/radiation effects , Retinal Degeneration/enzymology , Active Transport, Cell Nucleus/physiology , Active Transport, Cell Nucleus/radiation effects , Animals , Apoptosis/physiology , Cell Nucleus/enzymology , Cell Nucleus/ultrastructure , Disease Models, Animal , Gliosis/enzymology , Gliosis/pathology , Male , Mice , Mice, Inbred C3H , Mice, Inbred C57BL , Microscopy, Electron, Transmission , Mitochondria/enzymology , Mitochondria/radiation effects , Mitochondria/ultrastructure , Neurons/enzymology , Neurons/ultrastructure , Phosphorylation/radiation effects , Photic Stimulation/adverse effects , Photoreceptor Cells/enzymology , Photoreceptor Cells/pathology , Photoreceptor Cells/radiation effects , Rats , Rats, Sprague-Dawley , Retina/enzymology , Retina/pathology , Retinal Degeneration/etiology , Retinal Degeneration/pathology , Tyrosine/metabolism , WW Domain-Containing Oxidoreductase
11.
Cell Immunol ; 212(1): 35-43, 2001 Aug 25.
Article in English | MEDLINE | ID: mdl-11716527

ABSTRACT

Exposure to bacterial superantigens leads to the induction of a subsequent state of immune hyporesponsiveness. Using a transwell coculture system, a previous report demonstrated that splenocytes from staphylococcal enterotoxin B (SEB)-injected BALB/c mice secreted soluble mediators to suppress the proliferative response of naive syngeneic splenocytes to SEB stimulation. We show in the present study that, in contrast to the suppressive effect induced by SEB in BALB/c (H-2(d) haplotype), MRL(+/+), and MRL-lpr/lpr (H-2(k)) mice, SEB-primed splenocytes from I-E(-) strains such as B6, B10, A. BY (H-2(b)), and A.SW (H-2(s)) mice failed to inhibit the CD25 expression and the proliferative activity of their syngeneic naive responder splenocytes. Further results revealed that the SEB-primed cells from BALB/c, but not B6, mice inhibited the CD25 expression and proliferation of naive responder cells from either BALB/c or B6 mice, indicating the critical regulatory role of the effector cells. Unlike SEB, staphylococcal enterotoxin A induced profound suppression in both BALB/c and B6 mice. Moreover, the suppressive competence of SEB-primed splenocytes was diminished in CD28-deficient BALB/c mice. Taken together, our results indicate that when SEB is used as a stimulator in vivo, both the I-E molecule and CD28 costimulation are required for the induction of regulatory cells bearing suppressive activity.


Subject(s)
CD28 Antigens/immunology , Enterotoxins/immunology , Histocompatibility Antigens Class II/immunology , Staphylococcus aureus/immunology , Superantigens/immunology , Animals , Clonal Anergy/immunology , Coculture Techniques , Immunity , Mice , Mice, Inbred BALB C
12.
Mol Cell Neurosci ; 17(1): 141-50, 2001 Jan.
Article in English | MEDLINE | ID: mdl-11161475

ABSTRACT

Since recent reports have suggested that alpha-synuclein might play a role in neuronal plasticity, the main objective of this study was to determine the effects of alpha-synuclein on neuritic outgrowth. We stably transfected either human (h) alpha- or beta-synuclein cDNA in B103 rat neuronal cells. Expression of h(alpha)-synuclein resulted in reduced neurite extension and weak adhesion compared to vector-transfected and h(beta)-synuclein expressing cells. To investigate the potential pathways involved, we studied the effects of reagents known to modulate B103 proliferation and differentiation. Neither phorbol 12-myristate 13-acetate nor forskolin or antioxidants (catalase, superoxide dismutase, or vitamin E) were able to restore the reduced length of neurites in h(alpha)-synuclein-expressing cells. These results suggest that reduced neuritic activity in the h(alpha)-synuclein-expressing cells might be due, in part, to alterations in cell adhesion capacity. This might be attributed to alpha-synuclein affecting a signal transduction pathway distinct from protein kinase C and protein kinase A.


Subject(s)
Nerve Tissue Proteins/metabolism , Neurites/metabolism , Neurons/metabolism , Animals , Cell Adhesion/drug effects , Cell Differentiation/drug effects , Cell Line , Colforsin/pharmacology , Humans , Immunohistochemistry , Nerve Tissue Proteins/genetics , Nerve Tissue Proteins/pharmacology , Neurites/drug effects , Neurons/cytology , Neurons/drug effects , Rats , Synucleins , Tetradecanoylphorbol Acetate/pharmacology , Transfection , alpha-Synuclein , beta-Synuclein
13.
Am J Pathol ; 157(2): 401-10, 2000 Aug.
Article in English | MEDLINE | ID: mdl-10934145

ABSTRACT

Abnormal accumulation of the presynaptic protein alpha-synuclein has recently been implicated in the pathogenesis of Alzheimer's and Parkinson's diseases. Because neurodegeneration in these conditions might be associated with mitochondrial dysfunction and oxidative stress, the effects of alpha-synuclein were investigated in a hypothalamic neuronal cell line (GT1-7). alpha-Synuclein overexpression in these cells resulted in formation of alpha-synuclein-immunopositive inclusion-like structures and mitochondrial alterations accompanied by increased levels of free radicals and decreased secretion of gonadotropin-releasing hormone. These alterations were ameliorated by pretreatment with anti-oxidants such as vitamin E. Taken together these results suggest that abnormal accumulation of alpha-synuclein could lead to mitochondrial alterations that may result in oxidative stress and, eventually, cell death.


Subject(s)
Mitochondria/metabolism , Nerve Tissue Proteins/metabolism , Oxidative Stress , Animals , Gene Expression Regulation, Neoplastic , Glutathione/metabolism , Gonadotropin-Releasing Hormone/metabolism , Mice , Microscopy, Electron , Mitochondria/pathology , Nerve Tissue Proteins/genetics , RNA, Messenger/genetics , RNA, Messenger/metabolism , Synucleins , Tumor Cells, Cultured/cytology , Tumor Cells, Cultured/metabolism , Tumor Cells, Cultured/ultrastructure , alpha-Synuclein
14.
Cell Immunol ; 201(1): 50-7, 2000 Apr 10.
Article in English | MEDLINE | ID: mdl-10805973

ABSTRACT

Administration of bacterial superantigen results in clonal activation of T cells followed by a state of hyporesponsiveness to subsequent antigen stimulation. Using a coculture system, we showed that the splenocytes from staphylococcal enterotoxin B (SEB)-injected BALB/c mice suppressed the proliferative response of naive splenocytes to SEB stimulation. The suppressive effect also occurred in Fas-deficient MRL-lpr/lpr mice. When naive responder cells were separated by a semipermeable membrane from SEB-primed effector cells, the suppressive effect remained apparent. The hyporesponsiveness of responder cells did not result from excessive induction of apoptosis, but rather from prevention of entering the S and G2/M phases of the cell cycle. The IL-2 levels in culture supernatants were low with the presence of SEB-primed effector cells. However, addition of IL-2 to the cocultures only partially reversed the inhibitory effect. Further studies revealed a reduced level of the CD25(hi) subpopulation in responder cells when cultured in the transwell with the presence of SEB-primed effector cells compared to that with saline-primed controls. This inhibitory effect was not observed for SEB-induced activation of CD25(int) and CD69 expression. Taken together, using a transwell culture system, we show in this study an inhibition of CD25(hi) expression and cell cycle arrest in target cells, which may serve at least in part the mechanisms of SEB-induced hyporesponsiveness.


Subject(s)
Enterotoxins/immunology , Immunosuppressive Agents/immunology , Receptors, Interleukin-2/biosynthesis , Spleen/immunology , Staphylococcus aureus/immunology , Superantigens/immunology , Animals , Antigens, CD/metabolism , Antigens, Differentiation, T-Lymphocyte/metabolism , Apoptosis , Cell Cycle , Cell Division/drug effects , Cells, Cultured , Enterotoxins/administration & dosage , Fas Ligand Protein , Immunosuppression Therapy , Immunosuppressive Agents/administration & dosage , Interleukin-2/metabolism , Interleukin-2/pharmacology , Lectins, C-Type , Male , Membrane Glycoproteins/immunology , Mice , Mice, Inbred BALB C , Mice, Inbred MRL lpr , Recombinant Proteins/pharmacology , Spleen/cytology , Spleen/metabolism , Superantigens/administration & dosage , fas Receptor/genetics , fas Receptor/immunology
15.
J Biol Chem ; 274(41): 28849-52, 1999 Oct 08.
Article in English | MEDLINE | ID: mdl-10506125

ABSTRACT

alpha-Synuclein is a major component of aggregates forming amyloid-like fibrils in diseases with Lewy bodies and other neurodegenerative disorders, yet the mechanism by which alpha-synuclein is intracellularly aggregated during neurodegeneration is poorly understood. Recent studies suggest that oxidative stress reactions might contribute to abnormal aggregation of this molecule. In this context, the main objective of the present study was to determine the potential role of the heme protein cytochrome c in alpha-synuclein aggregation. When recombinant alpha-synuclein was coincubated with cytochrome c/hydrogen peroxide, alpha-synuclein was concomitantly induced to be aggregated. This process was blocked by antioxidant agents such as N-acetyl-L-cysteine. Hemin/hydrogen peroxide similarly induced aggregation of alpha-synuclein, and both cytochrome c/hydrogen peroxide- and hemin/hydrogen peroxide-induced aggregation of alpha-synuclein was partially inhibited by treatment with iron chelator deferoxisamine. This indicates that iron-catalyzed oxidative reaction mediated by cytochrome c/hydrogen peroxide might be critically involved in promoting alpha-synuclein aggregation. Furthermore, double labeling studies for cytochrome c/alpha-synuclein showed that they were colocalized in Lewy bodies of patients with Parkinson's disease. Taken together, these results suggest that cytochrome c, a well known electron transfer, and mediator of apoptotic cell death may be involved in the oxidative stress-induced aggregation of alpha-synuclein in Parkinson's disease and related disorders.


Subject(s)
Cytochrome c Group/metabolism , Lewy Body Disease/metabolism , Nerve Tissue Proteins/metabolism , Acetylcysteine/pharmacology , Antioxidants/pharmacology , Brain/pathology , Brain/ultrastructure , Cytochrome c Group/analysis , Deferoxamine/pharmacology , Humans , Hydrogen Peroxide/pharmacology , Immunohistochemistry , Lewy Body Disease/pathology , Nerve Tissue Proteins/analysis , Oxidative Stress , Recombinant Proteins/metabolism , Synucleins , alpha-Synuclein
16.
Neuroreport ; 10(4): 717-21, 1999 Mar 17.
Article in English | MEDLINE | ID: mdl-10208537

ABSTRACT

The precursor of non-amyloid beta protein component of Alzheimer's disease amyloid (NACP/alpha-synuclein), found in Lewy bodies of Parkinson's disease (PD), is a presynaptic protein genetically linked to some familial types PD. Mechanisms of abnormal NACP/alpha-synuclein aggregation in neurodegenerative diseases are unclear. Since oxidative stress might play a role in PD pathogenesis, we investigated the role of iron and peroxide in NACP/alpha-synuclein aggregation. Immunoblot analysis showed that human NACP/alpha-synuclein (but not beta-synuclein) aggregated in the presence of ferric ion and was inhibited by the iron chelator deferoxamine. Ferrous ion was not effective by itself, but it potentially aggregated NACP/alpha-synuclein in the presence of hydrogen peroxide. NACP/ alpha-synuclein aggregates displayed strong thioflavine-S and congo-red reactivity, reminiscent of amyloid. This study suggests that NACP/alpha-synuclein aggregation might be closely related to oxidative reactions which may play a critical role in neurodegeneration in disorders with Lewy bodies.


Subject(s)
Amyloid/chemistry , Nerve Tissue Proteins/chemistry , Oxidative Stress/physiology , Alzheimer Disease/metabolism , Amyloid/drug effects , Amyloid/ultrastructure , Catalysis , Chlorides , Ferric Compounds/chemistry , Ferrous Compounds/chemistry , Humans , Nerve Tissue Proteins/drug effects , Nerve Tissue Proteins/ultrastructure , Oxidation-Reduction , Recombinant Proteins/chemistry , Synucleins , alpha-Synuclein , beta-Synuclein
17.
J Neurochem ; 71(1): 338-44, 1998 Jul.
Article in English | MEDLINE | ID: mdl-9648883

ABSTRACT

The non-Abeta component of Alzheimer's disease amyloid precursor protein (NACP) is predominantly a neuron-specific presynaptic protein that may play a central role in neurodegeneration because NACP fragments are found in Alzheimer's disease amyloid and a mutation in the NACP gene is associated with familial Parkinson's disease. In addition, NACP may play an important role during synaptogenesis and CNS development. To understand better the patterns of NACP expression during development, we analyzed the levels of this protein as well as the levels of another synaptic protein (synaptophysin) by ribonuclease protection assay, western blotting, and immunocytochemistry in fetal, juvenile, and adult mouse brain. From embryonic day 12 to 15, there was a slight increase, which was then followed by a more dramatic increase at later time points. Immunocytochemical staining for NACP increases throughout these stages as well. Although NACP appeared early in CNS development, synaptophysin levels started to rise at a later stage. These findings support the contention that NACP might be important for CNS development. Furthermore, the cytosolic component of NACP precedes the particulate component in development, indicating that a redistribution of the protein to the membrane fraction may be important for events later in neuronal development and in synaptogenesis.


Subject(s)
Amyloid beta-Protein Precursor/metabolism , Amyloid/genetics , Brain/embryology , Gene Expression Regulation, Developmental/physiology , Nerve Tissue Proteins/genetics , Protein Precursors/genetics , Alzheimer Disease/metabolism , Amino Acid Sequence , Amyloid/analysis , Animals , Blotting, Western , Brain/metabolism , Brain Chemistry/physiology , Cell Membrane/chemistry , Cytosol/chemistry , Female , Mice , Mice, Inbred ICR , Molecular Sequence Data , Nerve Tissue Proteins/analysis , Pregnancy , Protein Precursors/analysis , RNA, Messenger/metabolism , Synaptic Vesicles/chemistry , Synaptic Vesicles/metabolism , Synaptophysin/analysis , Synaptophysin/genetics , Synucleins
18.
Brain Res ; 799(2): 301-6, 1998 Jul 20.
Article in English | MEDLINE | ID: mdl-9675319

ABSTRACT

The precursor of non-amyloid beta protein component of Alzheimer's disease amyloid (NACP/alpha-synuclein) is aggregated and fibrillated under certain conditions, i.e., increasing time lag, high temperature and low pH. These in vitro aggregates form Thioflavine-S-positive filamentous structures, reminiscent of amyloid-like fibrils. Since some Lewy bodies in Parkinson's disease display Thioflavine-S reactivity, our results may suggest that amyloidogenic properties of NACP/alpha-synuclein may play a crucial role in pathogenesis of disorders with Lewy bodies such as Parkinson's disease.


Subject(s)
Nerve Tissue Proteins/physiology , Benzothiazoles , Humans , Hydrogen-Ion Concentration , Nerve Tissue Proteins/ultrastructure , Osmolar Concentration , Parkinson Disease/etiology , Recombinant Proteins , Synucleins , Temperature , Thiazoles/metabolism , Time Factors
19.
Biochem Biophys Res Commun ; 237(3): 611-6, 1997 Aug 28.
Article in English | MEDLINE | ID: mdl-9299413

ABSTRACT

Non-amyloid-beta component precursor (NACP) is a presynaptic protein which may play a role in amyloidogenesis in Alzheimer's disease (AD). Since an abnormal function of platelets has been demonstrated in AD, platelets could be used as a model to investigate the role of NACP in this disease. We characterized the patterns of NACP and beta-synuclein expression in a megakaryocyte-platelet system (K562). In this hematopoietic cell line, NACP expression was up-regulated during phorbol ester-induced megakaryocytic differentiation, while beta-synuclein was down-regulated. Consistent with this, NACP but not beta-synuclein was abundantly expressed in platelets. Immunogold electron microscopy of platelets showed that NACP is loosely associated with the plasma membrane, the endomembrane system and, occasionally, with the membrane of secretory alpha-granules. These findings suggest that coordinate expression of the synuclein family members may play a critical role during hematopoietic cell differentiation. Additionally, expression of the synuclein family members may be developmentally regulated during neural differentiation.


Subject(s)
Amyloid/biosynthesis , Brain/metabolism , Gene Expression Regulation , Megakaryocytes/cytology , Nerve Tissue Proteins/biosynthesis , Protein Precursors/biosynthesis , Animals , Blood Platelets/cytology , Blood Platelets/metabolism , CHO Cells , Cell Differentiation/drug effects , Cell Membrane/metabolism , Cell Membrane/ultrastructure , Cricetinae , Cytoplasmic Granules/metabolism , Cytoplasmic Granules/ultrastructure , DNA Primers , Humans , Kinetics , Megakaryocytes/metabolism , Microscopy, Immunoelectron , Open Reading Frames , Polymerase Chain Reaction , Recombinant Proteins/biosynthesis , Synucleins , Tetradecanoylphorbol Acetate/pharmacology , Transfection , Tumor Cells, Cultured , beta-Synuclein
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